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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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177,602 results

Showing: 7361 - 7380 of 177602 results

  1. AIMTOR T757

    Plasmid
    #140828
    Purpose
    AIMTOR T757 contains a cytoplasmic mTOR Activity Reporter derived from hu ULK1 protein boxed between BRET-compatible entities (Ypet and Nanoluciferase) to measure mTOR activity in living cells
    Depositor
    Anne Bonnieu
    Publication
    Bouquier et al BMC Biol. 2020 Jul 3;18(1):81. doi: 10.1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AIMTOR T757 (ULK1 Human)
    Tags
    NES Nuclear export signal
    Expression
    Mammalian
    Mutation
    The insert comprises only a small sequence of hum…
    Available since
    July 10, 2020
    Availability
    Academic Institutions and Nonprofits only
    140828-plasmid-map-sequence-id-305141

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  4. pCC_05 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-dCas9-NLS-VPR-2A-Puro-WPRE

    Plasmid
    #139090
    Purpose
    Expresses human codon-optimized inactive SpCas9 fused to a transcriptional activator VPR in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.
    Depositor
    Neville Sanjana
    Publication
    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.
    Insert
    dSpCas9-VPR
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Available since
    March 6, 2020
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    139090-plasmid-map-sequence-id-273886

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  14. pLX_TRC311

    Plasmid
    #113668
    Purpose
    Gateway destination vector for lentiviral expression
    Depositor
    John Doench
    Type
    Empty backbone
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available since
    Sept. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    113668-plasmid-map-sequence-id-220922

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  18. pET28a-HaloTag7-SnoopLigase

    Plasmid
    #105627
    Purpose
    Plasmid for recombinant bacterial expression of SnoopLigase, a protein that promotes the formation of an isopeptide bond between 2 peptide tags (SnoopTagJr and DogTag), with N-terminal HaloTag7
    Depositor
    Mark Howarth
    Publication
    Buldun et al J Am Chem Soc. 2018 Feb 28;140(8):3008-3
    Insert
    HaloTag7-SnoopLigase
    Expression
    Bacterial
    Promoter
    T7
    Available since
    March 29, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    105627-plasmid-map-sequence-id-207217

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  19. pCH1/RAD51o

    Plasmid
    #102562
    Purpose
    Express Human RAD51 protein codon optimzed for E.coli and the E.coli GorE Operon
    Depositor
    Maria Spies
    Publication
    Subramanyam et al Proc Natl Acad Sci U S A. 2016 Oct 11;11
    Inserts
    Homo sapiens RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae) (RAD51), transcript variant 1 (RAD51 Synthetic, Human)
    E. coli groE operon
    Expression
    Bacterial
    Mutation
    Codon optimized for E.coli Expression
    Promoter
    T7
    Available since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    102562-plasmid-map-sequence-id-203161

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  20. pAAV.GFAP.eGFP.WPRE.hGH

    Plasmid
    #105549
    Purpose
    AAV expression of EGFP from GFAP promoter
    Depositor
    James M. Wilson
    Has service
    AAV5
    Insert
    eGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    GFAP
    Available since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    8 Articles
    105549-plasmid-map-sequence-id-202447

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Showing: 7361 - 7380 of 177602 results