We narrowed to 22,434 results for: ESC;

1. pLifeAct_mScarlet_N1

   Plasmid

   #85054

   Purpose

   In vivo visualization of actin cytoskeleton (can be used for colocalization studies)

   Depositor

   Dorus Gadella

   Article

   Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

   Insert

   LifeAct
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. RpHLuorin2

   Plasmid

   #180227

   Purpose

   for bacterial expression of recombinant ratiometric pHluorin2

   Depositor

   Geert van den Bogaart

   Article

   Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

   Insert

   Ratiometric pHLuorin2
   

   Tags

   His-tag followed by thrombin cleavage site

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   March 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSF11-wmiRFP680-T2A-HO1

   Plasmid

   #197244

   Purpose

   Expresses the protein of wmiRFP680-T2A-HO1 in neurons of C elegan

   Depositor

   Kiryl Piatkevich

   Article

   Zhang et al Nat Methods. 2023 Sep 4. doi: 10.1038/s4

   Insert

   wmiRFP680-T2A-HO1
   

   Expression

   Worm

   Available Since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pYS17

   Plasmid

   #202083

   Purpose

   Parent vector for producing universal minicircle donors for mNeonGreen N-terminal knock-in

   Depositor

   Daniel Dickinson

   Article

   Shi et al Development. 2023 May 15;150(10):dev2013

   Insert

   NT PuroR-T2A-mNG Minicircle Parent
   

   Use

   CRISPR

   Tags

   mNeonGreen

   Promoter

   None

   Available Since

   Oct. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-KIRIN1

   Plasmid

   #107414

   Purpose

   Bacterial Expression plasmid for potassium ion raitometric indicator KIRIN1

   Depositor

   Robert Campbell

   Article

   Shen et al Commun Biol. 2019 Jan 14;2:18. doi: 10.1

   Insert

   KIRIN1
   

   Expression

   Bacterial

   Available Since

   March 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. AAV-CAG-ChR2-GFP

   Plasmid

   #26929

   Purpose

   AAV-mediated expression of ChR2-GFP for neuronal excitation

   Depositor

   Edward Boyden

   Article

   Boyden et al Nat Neurosci. 2005 Sep . 8(9):1263-8.

   Insert

   ChR2
   

   Use

   AAV; Adeno-associated virus

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Jan. 11, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. pETNb-cALFA

   Plasmid

   #159989

   Purpose

   For the protein expression with ALFA tag at C-terminus in E. coli

   Depositor

   Motoyuki Hattori

   Article

   Jin et al Commun Biol. 2021 Mar 19;4(1):366. doi:

   Type

   Empty backbone

   Tags

   HRV3C protease cleavage site-ALFA tag-8histidine

   Expression

   Bacterial

   Available Since

   Dec. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pC28-LIC-sfGFP-His

   Plasmid

   #165479

   Purpose

   pET based vector for protein expression in E.coli for targets fused with C-terminal superfolder GFP and His tag

   Depositor

   Renato Bruni

   Article

   Bruni et al Curr Protoc Protein Sci. 2013 Nov 5;74:2

   Type

   Empty backbone

   Tags

   TEV-GFP-6xHis

   Expression

   Bacterial

   Available Since

   March 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1-mMaroon1

   Plasmid

   #83840

   Purpose

   Expresses mMaroon1

   Depositor

   Michael Lin

   Article

   Bajar et al Nat Methods. 2016 Oct 31. doi: 10.1038/n

   Insert

   mMaroon1
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCold CL7-Cas9

    Plasmid

    #124890

    Purpose

    obtaining full Cas9 RNP

    Depositor

    Yi Liu

    Article

    Qiao et al Commun Biol. 2019 May 3;2:161. doi: 10.1

    Insert

    Cas9
    

    Expression

    Bacterial

    Available Since

    Feb. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pTH2

    Plasmid

    #84452

    Purpose

    shuttle plasmid for sarA P1-CFP expression

    Depositor

    Reindert Nijland

    Article

    de Jong et al Sci Rep. 2017 Mar 7;7:43889. doi: 10.103

    Insert

    CFP Cerulean
    

    Use

    Shuttle vector e.coli-s.aureus, expression of sgf…

    Available Since

    Feb. 17, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR myc-MBP-SspB-iRFP-NLS

    Plasmid

    #223286

    Purpose

    An SspB-tagged maltose binding protein (MBP) with an infrared fluorescent protein for visualization. Used to demonstrate condensate dissolution using the OptoMBP system.

    Depositor

    Jared Toettcher

    Article

    Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

    Insert

    Myc-MBP-SspB-irFP-NLS
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 14, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. ERmoxGFP

    Plasmid

    #68072

    Purpose

    mammalian expression of ER localized moxGFP

    Depositor

    Erik Snapp

    Article

    Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

    Insert

    moxGFP
    

    Tags

    KDEL ER retention sequence and bovine prolactin s…

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 25, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pTOM20-ddGFP-B

    Plasmid

    #40291

    Depositor

    Robert Campbell

    Article

    Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

    Insert

    TOMM20 (TOMM20 Human)
    

    Tags

    ddGFP-B

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 21, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28a-MinE-His

    Plasmid

    #133623

    Purpose

    Expression vector for the Escherichia coli MinE with an C-terminal fusion of a His-tag for purification.

    Depositor

    Petra Schwille

    Article

    Glock et al ACS Synth Biol. 2018 Dec 20. doi: 10.102

    Insert

    MinE-His (minE E. coli)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    Feb. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET21a-LysRS-His

    Plasmid

    #124114

    Purpose

    Bacterial expression plasmid for PURE system components

    Depositor

    Sebastian Maerkl, Takuya Ueda

    Article

    Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

    Insert

    Lysine-tRNA ligase (lysU Escherichia coli)
    

    Tags

    6x-His

    Expression

    Bacterial

    Available Since

    April 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CAG.GLPLight1

    Plasmid

    #187467

    Purpose

    Expresses GLPLight1 in mammalian cells

    Depositor

    Tommaso Patriarchi

    Article

    Duffet et al Elife. 2023 Jun 2;12:RP86628. doi: 10.75

    Insert

    GLPLight1
    

    Use

    AAV

    Tags

    Flag tag

    Promoter

    CAG

    Available Since

    Feb. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pET-28c S2

    Plasmid

    #171920

    Purpose

    Express RNA-based (p)ppGpp sensor

    Depositor

    Mingxu You

    Article

    Sun et al Angew Chem Int Ed Engl. 2021 Nov 2;60(45

    Insert

    RNA-based (p)ppGpp sensor
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    July 15, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAT001

    Plasmid

    #171061

    Purpose

    Plasmid for expressing HIV-1 envelope glycoprotein

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Insert

    HIV-1 Env
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    July 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. Str-Ii_neomycin

    Plasmid

    #65312

    Purpose

    Cytoplasmic ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    Invariant chain (p33)
    

    Expression

    Mammalian

    Mutation

    Streptavidin in its cytoplasmid part

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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