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pEXPqcxip-hSURF4-FLAG

Plasmid

#159139

Purpose

Hu SURF4 -flag mammalian expression Gateway vector.

Depositor

Martin Dorf

Article

Li et al Immunity. 2011 Sep 23;35(3):426-40. Epub

Insert

surfeit 4 (SURF4 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV (cytomegalovirus) enhancer and the MSV (mouse…

Available since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(gGFP)-PGKmCherry2AGFP-W

Plasmid

#67982

Purpose

Cas9 activity reporter with mCherry and GFP

Depositor

Kosuke Yusa

Article

Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

Insert

U6gRNA cassette, PGKmCherry2AGFP cassette, WPRE

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Deleted BbsI site within WPRE

Promoter

Available since

Nov. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pWZL hygro Flag HA TRAP220 wt

Plasmid

#17433

Purpose

Retroviral expression of Med1/TRAP220 in mammalian cells

Depositor

Kai Ge

Article

Ge et al Mol Cell Biol. 2008 Feb . 28(3):1081-91.

Insert

TRAP220 (MED1 Human)

Use

Retroviral

Tags

Flag and HA

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 19, 2008

Availability

Academic Institutions and Nonprofits only

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IGF2BP1

Plasmid

#155676

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

IGF2BP1 (IGF2BP1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Sept. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti-X1-Neo-HA-UFL1

Plasmid

#139860

Purpose

Lentiviral expression of HA-tagged human UFL1

Depositor

Jacob Corn

Article

Liang et al Cell. 2020 Mar 19;180(6):1160-1177.e20.

Insert

UFL1 (UFL1 Human)

Use

Lentiviral

Tags

HA

Expression

Mutation

Promoter

Available since

March 24, 2020

Availability

Academic Institutions and Nonprofits only

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CPSF5

Plasmid

#155480

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CPSF5 (NUDT21 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLEGFP-WT-STAT3

Plasmid

#71450

Purpose

Retroviral expression of WT-STAT3. Please note that this plasmid contains WT-STAT3 tagged with FLAG, not GFP.

Depositor

George Stark

Article

Dasgupta et al J Biol Chem. 2014 Oct 31;289(44):30763-7

Insert

STAT3 (STAT3 Human)

Use

Retroviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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phINA

Plasmid

#132606

Purpose

Expresses human alpha-internexin

Depositor

Anthony Brown

Article

Stone et al Cytoskeleton (Hoboken). 2019 Jul;76(7-8)

Insert

INA (INA Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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tetO-MEF2C

Plasmid

#46031

Depositor

John Gearhart

Article

Addis et al J Mol Cell Cardiol. 2013 Apr 13;60C:97-1

Insert

MEF2C (MEF2C Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Mutation

Promoter

tetO

Available since

July 9, 2013

Availability

Academic Institutions and Nonprofits only

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pLAMP1-mCherry

Plasmid

#45147

Depositor

Amy Palmer

Article

Van Engelenburg et al Nat Methods. 2010 Apr;7(4):325-30. doi:

Insert

LAMP1-mCherry (LAMP1 Synthetic, Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

June 4, 2013

Availability

Academic Institutions and Nonprofits only

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pBABE-FLAG-LKB1

Plasmid

#8592

Depositor

Lewis Cantley

Article

Shaw et al Proc Natl Acad Sci U S A 2004 Mar 9;101(

Insert

LKB1 (STK11 Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

July 18, 2005

Availability

Academic Institutions and Nonprofits only

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pCAG/ATP8B2-HA

Plasmid

#209210

Purpose

Mammalian expression of ATP8B2

Depositor

Hye-Won Shin

Article

Takatsu et al J Biol Chem. 2011 Nov 4;286(44):38159-38

Insert

ATP8B2 (ATP8B2 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 27, 2023

Availability

Academic Institutions and Nonprofits only

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p6352 MSCV-CMV-CMV-Flag-HA-JMJD6

Plasmid

#31358

Depositor

Peter Howley

Article

Rahman et al Mol Cell Biol. 2011 Jul . 31(13):2641-52

Insert

JMJD6 (JMJD6 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pcDNA3-AKT-PH-GFP

Plasmid

#18836

Depositor

Craig Montell

Article

Kwon et al Mol Cell. 2007 Feb 23. 25(4):491-503.

Insert

AKT-PH domain (AKT1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

contains amino acids 1-148 (compared to GenBank r…

Promoter

CMV

Available since

July 14, 2008

Availability

Academic Institutions and Nonprofits only

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FLAG-PRMT3

Plasmid

#164695

Purpose

Expression of PRMT3 with an N-terminal FLAG tag

Depositor

Cheryl Arrowsmith

Article

Kaniskan et al Angew Chem Int Ed Engl. 2015 Apr 20;54(1

Insert

PRMT3 (PRMT3 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-N2-Frankenbody-HA

Plasmid

#175292

Purpose

Visualize endogeneous loci coupled with ZF probes harboring 10xHA tag

Depositor

Tim Stasevich

Article

Liu et al Genes Cells. 2021 Nov;26(11):905-926. do

Insert

anti-HA frankenbody fused with mCherry

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3 PARL-FLAG-CT wild type

Plasmid

#13639

Depositor

Luca Pellegrini

Article

Sik et al J Biol Chem. 2004 Apr 9. 279(15):15323-9

Insert

Presenilin-associated rhomboid-like (PARL, PARL Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA3.3-3XFLAG-AGO2

Plasmid

#136687

Purpose

Expresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)

Depositor

David Bartel

Article

McGeary et al Science. 2019 Dec 20;366(6472). pii: sci

Insert

argonaute RISC Catalytic Component 2 (AGO2 Human)

Use

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pLEX307-APN-blast

Plasmid

#158455

Purpose

Constitutive lentiviral expression of human APN.

Depositor

Alejandro Chavez, Sho Iketani

Insert

APN (ANPEP Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 10, 2020

Availability

Academic Institutions and Nonprofits only

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pDONR223-MAP4K4

Plasmid

#23486

Depositor

David Root, William Hahn

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

MAP4K4 (MAP4K4 Human)

Use

Gateway donor vector

Tags

Expression

Mutation

Promoter

Available since

July 30, 2010

Availability

Academic Institutions and Nonprofits only