We narrowed to 7,456 results for: Lif

1. GpACR1-pcDNA3.1-mScarlet

   Plasmid

   #204964

   Purpose

   Expression of GpACR1 in fusion with mScarlet in mammalian cells

   Depositor

   Peter Hegemann

   Article

   Oppermann et al eLife. 2024 Oct 14;12:RP90100. doi: 10.7

   Insert

   GpACR1
   

   Use

   Tags

   mScarlet

   Expression

   Mammalian

   Mutation

   Promoter

   CMV (+ enhancer)

   Available since

   Oct. 25, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-CAG-jAspSnFR3-mRuby3

   Plasmid

   #203459

   Purpose

   jAspSnFR3-mRuby3 in adenoviral expression vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3
   

   Use

   AAV and Adenoviral

   Tags

   His-tag and mRuby3

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Oct. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2-Opto-BMPR2a

   Plasmid

   #207616

   Purpose

   Zebrafish BMPR2a BMP receptor kinase domain and CTD fused to VfLOV

   Depositor

   Patrick Müller

   Article

   Rogers et al Elife. 2020 Nov 11;9:e58641. doi: 10.755

   Insert

   BMPR2a kinase domain and CTD + VfLOV domain (bmpr2a Vaucheria frigida, Zebrafish)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-RedO-shHsp90ab1(#c)

   Plasmid

   #206359

   Purpose

   AAV plasmid expressing HSP90AB1 shRNA in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   shRNA of Hsp90ab1 (#c)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-RedO-shHsp90ab1 (#a)

   Plasmid

   #206357

   Purpose

   AAV plasmid expressing HSP90AB1 shRNA in photoreceptors

   Depositor

   Connie Cepko

   Article

   Xue et al Elife. 2024 May 10;12:RP90749. doi: 10.7

   Insert

   shRNA #a of Hsp90ab1
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human red opsin

   Available since

   Aug. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR1a-jAspSnFR3-mRuby3-NLS

   Plasmid

   #203489

   Purpose

   Nuclear directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   jAspSnFR3-mRuby3-NLS
   

   Use

   Gateway entry vector

   Tags

   Nuclear localization signal and mRuby3

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR1a-MTS-jAspSnFR3-mRuby3

   Plasmid

   #203490

   Purpose

   Mitochondrial directed jAspSnFR3-mRuby3 in Gateway cloning entry vector

   Depositor

   Lucas Sullivan

   Article

   Davidsen et al eLife. 2024 Feb 23;12:RP90024. doi: 10.7

   Insert

   MTS-jAspSnFR3
   

   Use

   Gateway entry vector

   Tags

   His-tag, Mitochondrial localization signal, and m…

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pQIq_MRGS_HIS8_(27B3)_FLAG

   Plasmid

   #199613

   Purpose

   Encodes DARPin-FLAG 27B3 for bacterial expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-FLAG 27B3
   

   Use

   Tags

   8xHis and FLAG

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3.1_ E3_5 _hFc

   Plasmid

   #199617

   Purpose

   Encodes DARPin-hFc E3_5 for mammalian expression and column purification

   Depositor

   Shiva Tyagarajan

   Article

   Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

   Insert

   DARPin-hFc E3_5 (control)
   

   Use

   Tags

   hFc

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   May 1, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. NarCas9_AAV

    Plasmid

    #192145

    Purpose

    Expresses NarCas9?and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Wei et al Elife. 2022 Aug 12;11. pii: 77825. doi:

    Insert

    NarCas9
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCZGY2748

    Plasmid

    #193331

    Purpose

    Site specific CRISPR/Cas9 editing of C. elegans Chr I

    Depositor

    Yishi Jin

    Article

    Blazie et al Elife. 2021 Jul 29;10. pii: 68336. doi:

    Insert

    sgRNA for ttTi4348
    

    Use

    Tags

    None

    Expression

    Worm

    Mutation

    Promoter

    U6

    Available since

    Jan. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pPB UbC Arrestin-uTEV1-V5; UAS-mCherry

    Plasmid

    #173122

    Purpose

    Expresses uTEV1 and reporter receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    Arrestin-uTEV1-V5; UAS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG CAG CCL20-pre-mGRASP

    Plasmid

    #173114

    Purpose

    Expresses CCL20 sender construct in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    CCL20-pre-mGRASP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCDNA3 CMV myc-Arrestin-TEVp

    Plasmid

    #173115

    Purpose

    Expresses arrestin receiver construct in mammalian cells

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    myc-Arrestin-TEVp
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPB UbC Arrestin-TEVp(WT)-V5; UAS-mCherry

    Plasmid

    #173121

    Purpose

    Expresses TEVp(WT) and reporter receiver construct in DIPG

    Depositor

    Alice Ting

    Article

    Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

    Insert

    Arrestin-TEVp(WT)-V5; UAS-mCherry
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    UbC

    Available since

    Dec. 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET-His6-Sumo-CylK-Strep

    Plasmid

    #185962

    Purpose

    Expresses His6-Sumo-CylK-Strep in E. coli for refolding protein preparation

    Depositor

    Emily Balskus

    Article

    Braffman et al Elife. 2022 Feb 25;11. pii: 75761. doi:

    Insert

    CylK
    

    Use

    Tags

    His6-Sumo

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. TalO8-HSP30-CFP

    Plasmid

    #178304

    Purpose

    CoTrIP plasmid with HSP30 promoter driving CFP's expression

    Depositor

    Brian Zid

    Article

    Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

    Insert

    HSP30pr-CFP-tAdh1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HSP30 and NA

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. TalO8-HSP26-CFP

    Plasmid

    #178306

    Purpose

    CoTrIP plasmid with HSP26 promoter driving CFP's expression

    Depositor

    Brian Zid

    Article

    Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

    Insert

    HSP26pr-CFP-tAdh1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    HSP26 and NA

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. TalO8-GLC3-CFP

    Plasmid

    #178307

    Purpose

    CoTrIP plasmid with GLC3 promoter driving CFP's expression

    Depositor

    Brian Zid

    Article

    Chen et al eLife. 2022 Sep 15;11. pii: 76965. doi:

    Insert

    Glc3pr-CFP-tAdh1
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    GLC3 and NA

    Available since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Paxillin DN

    Plasmid

    #186151

    Purpose

    For expressing recombinant paxillin variants in bacteria.

    Depositor

    Michael Rosen

    Article

    Case et al Elife. 2022 Jan 20;11. pii: 72588. doi:

    Insert

    PXN (PXN Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Paxilln residues 1-321 added to N-terminus of WT …

    Promoter

    Lac

    Available since

    June 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. p8133 MSCV GFP Neo C-HA

    Plasmid

    #184522

    Purpose

    Expression of GFP

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Promoter

    MSCV LTR

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. p8130 MSCV GFP Puro C-FlagHA

    Plasmid

    #184521

    Purpose

    Expression of GFP

    Depositor

    Elizabeth White

    Article

    Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

    Insert

    GFP
    

    Use

    Retroviral

    Tags

    Flag, HA

    Expression

    Mutation

    Promoter

    MSCV LTR

    Available since

    June 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. NK85

    Plasmid

    #176611

    Purpose

    To minimize dimerization between FcRg transmembrane domains, as this cysteine forms a disulfide bridge between y chains. To manipulate ligand spacing.

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    FCERG
    

    Use

    Lentiviral

    Tags

    EGFP and HA, SNAP-tag

    Expression

    Mammalian

    Mutation

    C25A

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. NK83

    Plasmid

    #176610

    Purpose

    To measure Syk intensity in response to ligand clustering using DNA origami

    Depositor

    Meghan Morrissey

    Article

    Kern et al eLife. 2021 Jun 3;10. pii: 68311. doi: 1

    Insert

    NeonGreen-tSH2 Syk (Syk Mouse, Synthetic)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    n/a

    Promoter

    Available since

    May 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. Lenti-UbiC-GFP-ps

    Plasmid

    #183712

    Purpose

    GFP with palmitoylation signal

    Depositor

    Jeffrey Gerst

    Article

    Dasgupta et al Elife. 2023 May 30;12:e83584. doi: 10.75

    Insert

    GFP with palmitoylation signal
    

    Use

    Lentiviral

    Tags

    palmitoylation signal

    Expression

    Mutation

    Promoter

    UbiC

    Available since

    May 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. myr-palm-mEYFP-(L)-mCherry2-(L)-mEGFP-(L)-mApple

    Plasmid

    #182854

    Purpose

    Hetero-tetrmer expression vector with plasma membrane localization signal

    Depositor

    Salvatore Chiantia

    Article

    Dunsing et al Elife. 2021 Sep 8;10. pii: 69687. doi: 1

    Insert

    myr-palm-mEYFP-mCherry2-mEGFP-mApple
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. RP51A-No5'ss-NoBS-NoPseudoBS

    Plasmid

    #100987

    Purpose

    RP51A with T7 promoter in HindIII/EcoRI sites of pUC18. No 5'ss, No BS, No pseudo BS

    Depositor

    Aaron Hoskins

    Article

    Larson et al Elife. 2017 Aug 22;6. pii: 27592. doi: 1

    Insert

    RP51A (RPS17A Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    5'ss mut to C/ATACCT BS mut to GTTAGTG, pseu…

    Promoter

    Available since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pJH3137

    Plasmid

    #179617

    Purpose

    Punc-4::GCaMP6::RFP unc-54 3' UTR C.elegans A-MNs and others expression of GCaMP6::RFP

    Depositor

    Mei Zhen

    Article

    Gao et al Elife. 2018 Jan 23;7. pii: 29915. doi: 1

    Insert

    GCaMP6
    

    Use

    Tags

    RFP

    Expression

    Worm

    Mutation

    Promoter

    Punc-4

    Available since

    March 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. Cbrg-vab-1-RNAi

    Plasmid

    #175249

    Purpose

    RNAi feeding bacteria/plasmid to knock-down vab-1 in C. briggsae

    Depositor

    Anne Brunet

    Article

    Booth et al Elife. 2019 Jul 8;8. pii: 46418. doi: 10

    Insert

    vab-1
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. Cbrg-ceh-18-RNAi

    Plasmid

    #175250

    Purpose

    RNAi feeding bacteria/plasmid to knock-down ceh-18 in C. briggsae

    Depositor

    Anne Brunet

    Article

    Booth et al Elife. 2019 Jul 8;8. pii: 46418. doi: 10

    Insert

    ceh-18
    

    Use

    RNAi

    Tags

    Expression

    Mutation

    Promoter

    T7

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge