Addgene

We narrowed to 17,684 results for: Met

Showing: 1801 - 1850 of 17684 results

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pCasper4-QF#7-hsp70

Plasmid

#46135

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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SETMAR (3BO5)

Plasmid

#25250

Purpose

Bacterial expression for structure determination; may not be full ORF

Depositor

Cheryl Arrowsmith

Insert

SETMAR_11 (SETMAR Human)

Use

Tags

His

Expression

Bacterial

Mutation

Histone-lysine N-methyltransferase domain: Residu…

Promoter

Available since

Aug. 13, 2010

Availability

Industry, Academic Institutions, and Nonprofits
pAC-14-LEXA-QF

Plasmid

#46104

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

LexA-QF

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

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pCaSpeR-act5cB-QF#7m1

Plasmid

#46126

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pME-QS (L1-L2)

Plasmid

#61378

Purpose

Gateway Middle Entry Vector Containing QS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QS

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBdxs

Plasmid

#17821

Depositor

Jay Keasling

Article

Kim et al Biotechnol Bioeng. 2001 Feb 20. 72(4):40

Insert

dxs

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only
pSIR-bsr

Plasmid

#176507

Purpose

Self-inactivating retrovirus vector containing a Blasticidin S-resistance gene

Depositor

Hodaka Fujii

Article

Fujii et al Biol Methods Protoc. 2021 Dec 6;6(1):bpa

Type

Empty backbone

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-267

Plasmid

#68770

Purpose

Sensor pair 1 V1

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-terminal Sensor 1 V1
N-Terminal Sensor 1 V1

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD24MevT

Plasmid

#17820

Depositor

Jay Keasling

Article

Pitera et al Metab Eng. 2007 Mar . 9(2):193-207.

Insert

MevT

Use

Synthetic Biology

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 14, 2008

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-271

Plasmid

#68772

Purpose

Sensor pair 1 V3

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V3
N-Terminal Sensor 1 V3

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-246

Plasmid

#68760

Purpose

ZF9-TF-V1 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9-TF-V1
mCherry

Use

Tags

NLS and flag

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-160

Plasmid

#68751

Purpose

ZF10-ZF6 Op 0gap --GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF10/ZF6 Op 0gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-161

Plasmid

#68754

Purpose

ZF7-ZF8 Op 0gap -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF7/ZF8 Op 0gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBAD25

Plasmid

#231547

Purpose

L-arabinose inducible expression of heterologous genes, pBAD24-based backbone with KanR selective marker

Depositor

Martin Wiedmann

Article

Schumann et al J Microbiol Methods. 2025 Mar 18;232-234

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
RT-1

Plasmid

#220434

Purpose

Base construct used to create PUP2/3HA fusion proteins in S. cerevisiae. (pBSII/PUP2/3HA/URA3)

Depositor

Marvin Wickens

Article

Lapointe et al Nat Methods. 2015 Dec;12(12):1163-70. do

Type

Empty backbone

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-323

Plasmid

#68789

Purpose

Sensor pair 2 V2

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 2 V2
N-Terminal Sensor 2 V2

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-162

Plasmid

#68752

Purpose

ZF10-ZF6 Op 1gap -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF10/ZF6 Op 1gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-164

Plasmid

#68753

Purpose

ZF10-ZF6 Op 2gap -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF10/ZF6 Op 2gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-163

Plasmid

#68755

Purpose

ZF7-ZF8 Op 1gap -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF7/ZF8 Op 1gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL4.26-SS-165

Plasmid

#68756

Purpose

ZF7-ZF8 Op 2gap -- GFP

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Insert

ZF7/ZF8 Op 2gap

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pCFD5

Plasmid

#73914

Purpose

multiplex gRNA plasmid

Depositor

Simon Bullock

Article

Port et al Nat Methods. 2016 Oct;13(10):852-4. doi:

Insert

dU6-3:tRNA:gRNA:tRNA:gRNA

Use

CRISPR

Tags

Expression

Insect

Mutation

Promoter

dU6:3

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
mito-V5-APEX2

Plasmid

#72480

Purpose

Expresses APEX2 in mitochondria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

April 29, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3.1_crGE

Plasmid

#172933

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for mammalian cell expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFBDM

Plasmid

#110738

Purpose

Plasmid for the insect cell expression system, two promoters, Multiplication Module M, Tn7 transposition

Depositor

Tim Richmond

Article

Fitzgerald et al Nat Methods. 2006 Dec;3(12):1021-32. doi

Type

Empty backbone

Use

Tags

Expression

Insect

Mutation

Promoter

Polyhedrin and p10 promoters

Available since

June 21, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pFD152

Plasmid

#125546

Purpose

derivative of pFD116 with an optimized RBS for E. coli

Depositor

David Bikard

Article

Depardieu et al Methods. 2019 Aug 1. pii: S1046-2023(18)

Insert

optimized RBS

Use

CRISPR

Tags

Expression

Mutation

Promoter

anhydrotetracycline Ptet promoter

Available since

Aug. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMEV4

Plasmid

#110539

Purpose

The plasmid serves as a backbone for heterologous expression in the model archaeal organism Methanococcus maripaludis.

Depositor

William B. Whitman

Article

Lyu et al ACS Synth Biol. 2016 Jul 15;5(7):577-81.

Type

Empty backbone

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Aug. 28, 2018

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
pTRC-APEX2

Plasmid

#72558

Purpose

Expression of cytosolic APEX2 in bacteria

Depositor

Alice Ting

Article

Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

Insert

APEX2

Use

Tags

His6

Expression

Bacterial

Mutation

A134P relative to APEX

Promoter

Available since

May 2, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pGAL-GFP kan

Plasmid

#122060

Purpose

galactose inducible expression of GFP in yeast. marked with URA3

Depositor

Daniel Reines

Article

Reines et al Methods. 2019 Jan 4. pii: S1046-2023(18)

Insert

green fluorescent protein

Use

Tags

Expression

Yeast

Mutation

Promoter

GAL1

Available since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pJKR-H-benM

Plasmid

#73925

Purpose

Muconate biosensor. Expresses GFP proportional to muconate concentration within the cell.

Depositor

George Church

Article

Rogers et al Proc Natl Acad Sci U S A. 2016 Feb 8. pi

Insert

muconate biosensing apparatus

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 14, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAG4

Plasmid

#70115

Purpose

Tn7-based lux reporter for promoter cloning in bacteria

Depositor

Thomas Lewis

Article

Glassing et al J Microbiol Methods. 2015 Sep 1;118:75-7

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

none

Available since

Dec. 21, 2015

Availability

Academic Institutions and Nonprofits only
pRSETA_crGE

Plasmid

#172927

Purpose

Crowding FRET sensor with mCerulean3 and mCitrine for E. coli expression. Linker comprises 2 helices and 3 GSG domains.

Depositor

Arnold Boersma

Article

Boersma et al Nat Methods. 2015 Mar;12(3):227-9, 1 p f

Insert

CrowdingSensorGE

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

T7

Available since

Aug. 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
A1-20B1(P004)

Plasmid

#184056

Purpose

This plasmid carries Yn situ preamplifier A1-20B1 sequence and is used for nickase based synthesis.

Depositor

C. Ron Yu

Article

Wu et al Cell Rep Methods. 2022 Apr 18;2(4):10020

Insert

A1-20B1 preamplifier

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 11, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pVITRO1-SS-345

Plasmid

#68785

Purpose

R->A Sensor pair 1 with mCherry marker

Depositor

James Collins

Article

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

C-Terminal Sensor 1 V2 R-->A mutant
N-Terminal Sensor 1 V2 R-->A mutant
mCherry

Use

Tags

NLS, flag, and nls

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv-mCherry-10xPP7

Plasmid

#185796

Purpose

mCherry labeled with 10xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-10xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pFGC5941-PacI

Plasmid

#44182

Depositor

Guiliang Tang

Article

Tang et al Methods. 2012 Oct;58(2):118-25. doi: 10.

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-7-QFBDAD-hsp70

Plasmid

#46115

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
piRFP702-N1

Plasmid

#45456

Depositor

Vladislav Verkhusha

Article

Shcherbakova et al Nat Methods. 2013 Jun 16. doi: 10.1038/n

Insert

iRFP702

Use

Tags

Expression

Mammalian

Mutation

T2A, S45T, D124E, M170L, D202L, I203V, I219V, R23…

Promoter

CMV

Available since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
p5E-QUAS (L4-R1)

Plasmid

#61374

Purpose

5' Gateway Entry Vector Containing QUAS

Depositor

Marnie Halpern

Article

Subedi et al Methods. 2014 Apr 1;66(3):433-40. doi: 1

Insert

QUAS

Use

Tags

Expression

Mutation

Promoter

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pBabe-SIRT2

Plasmid

#66144

Purpose

Expresses SIRT2 via retroviral method

Depositor

Ronald Kahn

Article

Jing et al Cell Metab. 2007 Aug . 6(2):105-14.

Insert

SIRT2 (Sirt2 Mouse)

Use

Retroviral

Tags

3xFLAG

Expression

Mammalian

Mutation

E28G

Promoter

Available since

July 18, 2016

Availability

Academic Institutions and Nonprofits only
pSM040

Plasmid

#128294

Purpose

asymmetric plasmid partitioning E. coli - target plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Piq - mRFP, parS

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

July 26, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAZe

Plasmid

#60877

Purpose

Reporter construct for lineage tracing and mosaic analysis in zebrafish.

Depositor

Julian Lewis

Article

Collins et al Nat Methods. 2010 Mar;7(3):219-23. doi:

Inserts

hsp70l promoter
Cre
dTomato
UAS promoter
Gal4VP16

Use

Zebrafish expression

Tags

Expression

Mutation

Promoter

Available since

March 9, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge
pUC-STS

Plasmid

#35949

Depositor

Claudia Schmidt-dannert

Article

Watts et al BMC Biotechnol. 2006 Mar 21;6:22.

Insert

Stilbene synthase

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

LacPMod

Available since

June 15, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge
cmv-mCherry-8xPP7

Plasmid

#185795

Purpose

mCherry labeled with 8xPP7

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-mCherry-8xPP7

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM019

Plasmid

#128291

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-sfyfp-parB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
sZF15

Plasmid

#43846

Depositor

George Church

Article

Mali et al Nat Methods. 2013 Mar 17. doi: 10.1038/n

Insert

sZF15

Use

Tags

HA Flag and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 11, 2013

Availability

Academic Institutions and Nonprofits only
pCasper4-tubulin-IVS-Syn21-QSco-p10

Plasmid

#46132

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QSco

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pSM015

Plasmid

#128289

Purpose

asymmetric plasmid partitioning E. coli - regulatory plasmid

Depositor

Matthew Bennett

Article

Molinari et al Nat Chem Biol. 2019 Sep;15(9):917-924. d

Insert

Para - lp-cfp-parB

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pattB-synaptobrevin-7m1-QFBDADM1-hsp70

Plasmid

#46116

Depositor

Christopher Potter

Article

Riabinina et al Nat Methods. 2015 Jan 12. doi: 10.1038/n

Insert

QF#7m1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Aug. 12, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pMAL-c2x-MT1

Plasmid

#32101

Depositor

David DeRosier, Chris Mercogliano, Cristina Risco Ortiz

Article

Mercogliano et al J Struct Biol. 2007 Oct;160(1):70-82. Ep

Insert

Metallothionein (Mt1 Mouse)

Use

Tags

MBP

Expression

Bacterial

Mutation

Promoter

P-lac

Available since

Jan. 13, 2012

Availability

Academic Institutions and Nonprofits only
peZinCh-NB

Plasmid

#24173

Depositor

Maarten Merkx

Article

Vinkenborg et al Nat Methods. 2009 Oct;6(10):737-40. doi:

Insert

eZinCh-NB

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2010

Availability

Academic Institutions and Nonprofits only

Enlarge

Showing: 1801 - 1850 of 17684 results

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