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pCMV-mTagBFP2-PLCδ-PH

Plasmid

#214275

Purpose

Mammalian expression of blue fluorescent PI(4,5)P2 reporter mTagBFP2-PLCδ-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-PLCδ(1-175) (PLCD1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-sadCas9-VP64

Plasmid

#115790

Purpose

An AAV vector expressing S. aureus dCas9 fused to VP64.

Depositor

Nadav Ahituv

Article

Matharu et al Science. 2018 Dec 13. pii: science.aau06

Insert

Sa-dCas9

Use

AAV

Tags

NLS and NLS-VP64

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-AcGFP1-ORP9-PH

Plasmid

#214266

Purpose

Mammalian expression of green fluorescent PI4P reporter AcGFP1-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

AcGFP1-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT RAD23B

Plasmid

#201459

Purpose

Expresses human RAD23B with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

UV excision repair protein RAD23 homolog B (RAD23B Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-iRFP713-ORP9-PH

Plasmid

#214269

Purpose

Mammalian expression of near-IR fluorescent PI4P reporter iRFP713-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

iRFP713-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-5U-Venus-PSD-95-3U

Plasmid

#102949

Purpose

Expresses Venus-PSD-95 in mammalian cells

Depositor

Gary Bassell

Article

Ifrim et al J Neurosci. 2015 May 6;35(18):7116-30. d

Insert

PSD-95 (Dlg4 Mouse)

Use

Tags

Venus Fluorescent protein

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-dTomato-ORP9-PH

Plasmid

#214268

Purpose

Mammalian expression of red fluorescent PI4P reporter dTomato-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

dTomato-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV6-AN-HA_WT REV1

Plasmid

#201586

Purpose

Expresses human DNA repair protein REV1 with an N-terminal HA tag in mammalian cells

Depositor

Roger Woodgate

Article

Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

Insert

DNA repair protein REV1 (REV1 Human)

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-Myc-FOXA1

Plasmid

#219394

Purpose

Express FOXA1 in mammalian cells

Depositor

Yoichi Furukawa

Article

Nakagawa et al Commun Biol. 2024 Apr 29;7(1):510. doi:

Insert

FOXA1 (FOXA1 Human)

Use

Tags

N terminal Myc tag

Expression

Mammalian

Mutation

Promoter

Available since

June 17, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-mTagBFP2-ORP9-PH

Plasmid

#214267

Purpose

Mammalian expression of blue fluorescent PI4P reporter mTagBFP2-ORP9-PH

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

mTagBFP2-ORP9(1-111) (Osbpl9 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV_FLAG-NFL(R438TAG)

Plasmid

#182662

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position R438 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a R438TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

R438TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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myc EDC4 pCMVSport6

Plasmid

#86855

Purpose

mammalian expression of EDC4

Depositor

Sarah Slavoff

Article

D'Lima et al Nat Chem Biol. 2016 Dec 5. doi: 10.1038/

Insert

EDC4 (EDC4 Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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pCMV-mGold-Tubulin-C-18

Plasmid

#158008

Purpose

Visualization of tubulin in mammalian cells using mGold

Depositor

Francois St-Pierre

Article

Lee et al Sci Adv. 2020 Oct 23;6(43). pii: 6/43/ea

Insert

Tubulin (TUBA1B Human)

Use

Tags

mGold

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV E2F1 E132

Plasmid

#24224

Purpose

Mammalian expression of human E2F1 (DNA binding mutant) with HA tag

Depositor

Kristian Helin

Article

Helin et al Genes Dev. 1993 Oct . 7(10):1850-61.

Insert

E2F1 E132 (E2F1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2011

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM3-C71,72,105A

Plasmid

#58396

Purpose

Expressed murine IFITM3-C71,72,105A with an N-terminal HA tag in mammalian cells

Depositor

Jacob Yount, Howard Hang

Article

Yount et al Nat Chem Biol. 2010 Aug;6(8):610-4. doi:

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 71, 72, and 105 to Alanine

Promoter

CMV IE

Available since

Sept. 4, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-DNAJC5 (WT)-HaloTag

Plasmid

#205710

Purpose

Mammalian expression of DNAJC5 (WT)-HaloTag

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

DNAJC5 (DNAJC5 Human)

Use

Tags

HaloTag

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-Parkin-A92mKO2

Plasmid

#213548

Purpose

Transient mammalian expresssion of Parkin, internally tagged with mKO2

Depositor

Lani Wu, Steven Altschuler

Article

Waters et al Cell Rep. 2023 Oct 31;42(10):113260. doi

Insert

Parkin (PRKN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV_FLAG-NFL(K211TAG)

Plasmid

#182660

Purpose

Expresses mouse neurofilament light chain with a TAG codon at the position K211 and an N-terminal FLAG tag (DYKDDDDK) in mammalian cells.

Depositor

Ivana Nikić-Spiegel

Article

Arsic et al Nat Commun. 2022 Jan 14;13(1):314. doi:

Insert

mouse neurofilament light chain with a K211TAG mutation (Nefl Mouse)

Use

Tags

FLAG tag (DYKDDDDK)

Expression

Mammalian

Mutation

K211TAG

Promoter

CMV

Available since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pCMV-HA-mIFITM3-K24,83,88,104A

Plasmid

#58408

Purpose

Expresses murine IFITM3-K24,83,88,104A with an N-terminal HA tag in mammalian cells (Lysine-less nonubiquitinated mutant)

Depositor

Jacob Yount, Howard Hang

Article

Yount et al J Biol Chem. 2012 Jun 1;287(23):19631-41

Insert

IFITM3 (Ifitm3 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Lysines 24, 83, 88, and 104 to Alanine

Promoter

CMV IE

Available since

Sept. 8, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-HA-mIFITM1-C49,50,83,103A

Plasmid

#58421

Purpose

Expresses murine IFITM1-C49,50,83,103A with an N-terminal HA tag in mammalian cells (cysteine-less mutant that is not palmitoylated).

Depositor

Jacob Yount

Article

Hach et al J Virol. 2013 Sep;87(17):9923-7. doi: 10

Insert

IFITM1 (Ifitm1 Mouse)

Use

Tags

HA

Expression

Mammalian

Mutation

Cysteines 49, 50, 83, and 103 to Alanine

Promoter

CMV IE

Available since

Sept. 9, 2014

Availability

Academic Institutions and Nonprofits only