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pMSCV-MAVS-M142A(KaganE19)

Plasmid

#52010

Purpose

Expresses the human MAVS CDS containing the point mutation M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M1A,M142A(KaganF62)

Plasmid

#52002

Purpose

Expresses the human MAVS CDS containing two point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-MAVS-M142A(KaganF59)

Plasmid

#52062

Purpose

Expresses the human MAVS CDS containing the point mutation M142A "weak translational context"

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M142A

Expression

Mammalian

Mutation

changed Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pMSCV-MAVS-M1A,M142A(KaganE20)

Plasmid

#52011

Purpose

Expresses the human MAVS CDS containing the point mutations M1A and M142A

Depositor

Jonathan Kagan

Article

Brubaker et al Cell. 2014 Feb 13;156(4):800-11. doi: 10

Insert

MAVS-M1A,M142A

Use

Retroviral

Expression

Mammalian

Mutation

changed Met 1 to Ala and Met 142 to Ala

Available Since

March 24, 2014

Availability

Academic Institutions and Nonprofits only
pQC_HA-p115 TEV-Myc #77#407

Plasmid

#42127

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Mutation

inserted ENLYFQ b/w 886 and 887

Promoter

CMV

Available Since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pcDNA3.1_HA-p115-TEV-cleaved #957#382

Plasmid

#42107

Depositor

Ayano Satoh

Article

Koreishi et al Antibody Technology Journal. 2011;1:5-11

Insert

p115

Tags

HA

Expression

Mammalian

Mutation

deleted 886 to end and added ENLYFQ

Promoter

CMV

Available Since

Feb. 20, 2013

Availability

Academic Institutions and Nonprofits only
Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIA II

Plasmid

#52093

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with mAmetrine

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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Murine CD3 WTdelta-F2A-gamma-T2A-epsilon-P2A-zeta pMIG II

Plasmid

#52092

Purpose

Retroviral expression of all four WT CD3 subunits, co-expressed with EGFP

Depositor

Dario Vignali

Article

Holst et al Nat Immunol. 2008 Jun;9(6):658-66. doi:

Inserts

CD3 delta (Cd3d Mouse)
CDC gamma (Cd3g Mouse)
CD3 epsilon (Cd3e Mouse)
CD3 zeta (Cd247 Mouse)

Use

Retroviral

Tags

F2A, IRES-EGFP, P2A, and T2A

Expression

Mammalian

Available Since

April 9, 2014

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-N_NLE1:1-485

Plasmid

#211141

Purpose

Mammalian expression of full-length human NLE1. N-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NLE1:M1-R485 (NLE1 Human)

Tags

MVSGWRLFKKISGSSGGSSG

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pBiT3.1-C_NLE1:1-485

Plasmid

#211182

Purpose

Mammalian expression of full-length human NLE1. C-terminal HiBiT tag.

Depositor

Cheryl Arrowsmith

Article

Ackloo et al J Med Chem. 2025 Jan 23;68(2):1092-1112.

Insert

NLE1:M1-R485 (NLE1 Human)

Tags

GSSGGSSGVSGWRLFKKIS

Expression

Mammalian

Mutation

P6A natural variant (VAR_060327). R169K natural v…

Promoter

CMV

Available Since

March 19, 2024

Availability

Academic Institutions and Nonprofits only

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pNP2922 [pNP2321 - Cloning backbone, bRNA Landing Pad, with Ef1a-ISCro4 Recombinase]

Plasmid

#247255

Purpose

Cloning backbone for cloning bridge RNAs for ISCro4

Depositor

Patrick Hsu

Article

Perry et al Science. 2025 Sep 25:eadz0276. doi: 10.1

Type

Empty backbone

Expression

Mammalian

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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HLA_DRA_01_01

Plasmid

#165012

Purpose

Expression of HLA-DRA*01:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRA (HLA-DRA Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_04_01

Plasmid

#165013

Purpose

Expression of HLA-DRB1*04:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*04:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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HLA_DRB1_07_01

Plasmid

#165014

Purpose

Expression of HLA-DRB1*07:01 cDNA

Depositor

Matthew Meyerson

Article

Lee et al Sci Immunol. 2021 Jan 22;6(55). pii: 6/5

Insert

HLA-DRB1*07:01 (HLA-DRB1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

March 17, 2021

Availability

Academic Institutions and Nonprofits only

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pmScarlet3_C1

Plasmid

#189753

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV GFP1-9

Plasmid

#182244

Purpose

Tripartite split-GFP mammalian. Express GFP1-9 detector fragment

Depositor

Stéphanie Cabantous

Article

Cabantous et al Sci Rep. 2013 Oct 4;3:2854. doi: 10.1038

Insert

GFP 1-9 OPT

Expression

Mammalian

Promoter

CMV

Available Since

April 15, 2022

Availability

Academic Institutions and Nonprofits only

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pmScarlet-I3_C1

Plasmid

#189756

Purpose

C1 Cloning vector for mammalian cells incorporating mScarlet-I3 red fluorescent protein

Depositor

Dorus Gadella

Article

Gadella TWJ et al Nat Methods. 2023 Apr;20(4):541-545. doi

Insert

mScarlet-I3

Expression

Mammalian

Promoter

CMV

Available Since

March 21, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti_EF1a_EV

Plasmid

#183116

Purpose

Empty vector control for pLenti_EF1a constructs

Depositor

William Kaelin

Article

Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

Insert

Small multiple cloning site

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 24, 2022

Availability

Academic Institutions and Nonprofits only

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pICH47742:: pRPS5a-PE2max-NC::tHSP

Plasmid

#227694

Purpose

Golden Gate level 1, position 2, PE2max-NC expression cassette for Arabidopsis.

Depositor

Jae-Yean Kim

Article

Vu et al Nat Plants. 2024 Sep 6. doi: 10.1038/s41

Insert

PE2max-NC being driven by pRPS5a and terminated by tHSP

Use

CRISPR

Expression

Plant

Promoter

pRPS5a

Available Since

Nov. 14, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Flag Rac2

Plasmid

#12192

Depositor

Gary Johnson

Article

Abell et al J Cell Sci. 2004 Jan 15. 117(Pt 2):243-5

Insert

Rac2 (RAC2 Human)

Tags

Flag

Expression

Mammalian

Available Since

Oct. 3, 2006

Availability

Academic Institutions and Nonprofits only

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