We narrowed to 10,337 results for: plasmids 101

1. pLV-tetO-Id2

   Plasmid

   #70764

   Purpose

   Lentiviral plasmid for tetracycline/doxycycline dependent expression of murine Id2

   Depositor

   Hubert Schorle

   Article

   Kubaczka et al Cell Stem Cell. 2015 Sep 22. pii: S1934-

   Insert

   inhibitor of DNA binding 2 (Id2 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   tetO

   Available Since

   Nov. 16, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pmiRGLO-miR-124-sponge (pos. CTRL)

   Plasmid

   #117327

   Purpose

   Dual luciferase assay positive control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. Linked Free NES

   Plasmid

   #182485

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-AcNES1, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. Linked Free PcPTS

   Plasmid

   #182486

   Purpose

   Yeast integrative plasmid for expressing fusion protein ERG20-PcPTS, with linker sequence AG4TGGA (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3)

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Insert

   FPPS-PTS
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10

   Available Since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pmiRGLO-miR-124-sponge_rev(neg. CTRL)

   Plasmid

   #117326

   Purpose

   Dual luciferase assay negative control for miR-124 binding

   Depositor

   Volker Busskamp

   Article

   Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

   Insert

   reverse miR-124 sponge
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   Promoter

   Murine PGK-1

   Available Since

   Dec. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. sgRNA BRCA1 C64Y

   Plasmid

   #139326

   Purpose

   Plasmid expressing a sgRNA to introduce BRCA1 C64Y using base editing

   Depositor

   Alberto Ciccia

   Article

   Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

   Insert

   sgRNA to insert BRCA1 C64Y using base editing
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. tetO-ESRRG

   Plasmid

   #170684

   Purpose

   doxycycline-inducible overexpression of ESRRG

   Depositor

   Paul Burridge

   Article

   Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

   Insert

   ESRRG (ESRRG Human)
   

   Use

   Lentiviral; Doxycycline inducible

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   TRE promoter, Tet-ON

   Available Since

   July 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHAGE2-TetOminiCMV-Klf4

   Plasmid

   #136613

   Purpose

   Dox-inducible lentiviral vector expressing mouse Klf4

   Depositor

   Hans Schöler

   Article

   Velychko et al Cell Stem Cell. 2019 Oct 30. pii: S1934-

   Insert

   Klf4 (Klf4 Mouse)
   

   Use

   Lentiviral

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   tetO-miniCMV (dox-inducible)

   Available Since

   March 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet21a-aGFPnb-enhancer-cys-linker-YbbR-(PAS)5-H6

   Plasmid

   #192788

   Purpose

   aGFP nanobody enhancer tagged with cysteine, YbbR and H6 for bacterial expression, purification and labeling

   Depositor

   Jacob Piehler

   Article

   Sotolongo Bellon et al Cell Rep Methods. 2022 Feb 4;2(2):100165

   Insert

   antiGFP nanobody enhancer
   

   Use

   Tags

   H6 and ybbR

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAVS1-A2UCOE-EF1a-iCasp9-P2A-HLA-E SCT-T2A-hCD55-E2A-neo-AAVS1

    Plasmid

    #205444

    Purpose

    human AAVS1-targeting plasmid for expression of human CD46, HLA-G SCT, CD47, and CD59

    Depositor

    Deepta Bhattacharya

    Article

    Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

    Insert

    iCasp9-HLA-E SCT-hCD55
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    Sept. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. AAV2_hSyn_Phobos_Citrine

    Plasmid

    #98216

    Purpose

    Blue-shifted artificial anion conducting channelrhodopsin (aACR). Activation max 466 nm; off-kinetics 10 ms. Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct Phobos gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    T59S, E83N, E90Q, E101S, V117R, E123S, T159G, G16…

    Promoter

    human synapsin

    Available Since

    Aug. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRP[2CRISPR]-mCherry/Hygro-hCas9-U6>{mdx left}-U6>{mdx right}

    Plasmid

    #184381

    Purpose

    This plasmid contains hCas9 and two gRNAs that can excise the genomic area around the mouse mdx mutation in dystrophin's exon 23

    Depositor

    Ori Bar-Nur

    Article

    Domenig et al Stem Cell Reports. 2022 Feb 8;17(2):321-

    Insert

    Cas9, two gRNAs targeting dystrophin
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. sgRNA BRCA1 Q1779Stop

    Plasmid

    #139331

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 Q1779Stop using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 Q1779Stop using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. sgRNA BRCA1 T922I

    Plasmid

    #139328

    Purpose

    Plasmid expressing a sgRNA to introduce BRCA1 T922I using base editing

    Depositor

    Alberto Ciccia

    Article

    Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

    Insert

    sgRNA to insert BRCA1 T922I using base editing
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    May 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. B-HeFSpCas9

    Plasmid

    #126766

    Purpose

    Expression plasmid for human codon-opt. increased fidelity Blackjack-HeFSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than HeFSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-HeFSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    N497A; R661A; Q695A; K848A; Q926A; K1003A; R1060A…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPRIME-CMV-GFP-shDyn2

    Plasmid

    #132712

    Purpose

    Encodes short hairpin RNA (shRNA) #2 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(2) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGL3-BASIC- ARHGAP29 Mutant PROMOTER

    Plasmid

    #104156

    Purpose

    luciferase reporter with ARHGAP29 mutant promoter

    Depositor

    Marius Sudol

    Article

    Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

    Insert

    ARHGAP29 promoter (ARHGAP29 Human)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Mutation

    delete two TEAD binding sites

    Promoter

    ARHGAP29

    Available Since

    Jan. 2, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-FLEXfrt-SaCas9-U6-sgGria3

    Plasmid

    #124869

    Purpose

    Mutagenesis of Gria3

    Depositor

    Larry Zweifel

    Article

    Hunker et al Cell Rep. 2020 Mar 24;30(12):4303-4316.e

    Insert

    Gria3 (Gria3 Mouse)
    

    Use

    AAV, CRISPR, and Mouse Targeting

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pPRIME-CMV-GFP-shDyn1

    Plasmid

    #132711

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Pdyn gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shDyn(1) (Pdyn Rat)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. B-eSpCas9

    Plasmid

    #126761

    Purpose

    Expression plasmid for human codon-optimized increased fidelity Blackjack-eSpCas9 (w/o U6-sgRNA). Cleaves both 20nt and 5'-extended 21nt spacer containing sgRNAs with higher fidelity than eSpCas9.

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2020 Mar 6;11(1):1223. doi:

    Insert

    B-eSpCas9
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    K848A; K1003A; R1060A; amino acids 1005-1013 repl…

    Promoter

    CBh

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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