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pHAGE2-EF1a-MCS-hPOT1_K90E_IRES-blast

Plasmid

#166412

Purpose

express hPOT1 K90E in mammalian cells

Depositor

Agnel Sfeir

Article

Pinzaru et al Genes Dev. 2020 Dec 1;34(23-24):1619-163

Insert

hPOT1 K90E (POT1 Human)

Use

Lentiviral

Tags

MYC

Expression

Mammalian

Mutation

K90E

Promoter

EF-1a

Available Since

May 6, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-NNQ-HA-GFP

Plasmid

#121164

Purpose

Expresses mmPtch1-B NNQ variant

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, N…

Promoter

CMV

Available Since

Jan. 31, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-I766FV111FL114F-HA-GFP

Plasmid

#120909

Purpose

Expresses Ptch1-B (I766FV111FL114F)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

GFP and HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-L235C-HA

Plasmid

#120906

Purpose

Expresses mmPtch1-B (L235C)

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291, L…

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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LentiCRISPRv2-sgSORCS2-G2

Plasmid

#118416

Purpose

CRISPR knockout, expresses Cas9, and sgRNA targeting Human SORCS2

Depositor

Mark Denham

Article

Hojland Knudsen et al Front Cell Dev Biol. 2018 May 15;6:54. d

Insert

gRNA SORCS2 Human (SORCS2 Human)

Use

CRISPR and Lentiviral

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pLenCMV-PMMars1CyAVPR2

Plasmid

#75226

Purpose

Lentiviral transfer vector encoding Mars1Cy-tagged AVPR2

Depositor

Alan Waggoner

Article

Zhang et al J Clin Invest. 2015 Oct 1;125(10):3915-2

Insert

Mars1Cy-AVPR2 (AVPR2 Synthetic)

Use

Lentiviral

Tags

HA epitope

Expression

Mammalian

Promoter

CMV

Available Since

June 1, 2016

Availability

Academic Institutions and Nonprofits only

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GST-PC-2-CTS

Plasmid

#86068

Purpose

A bacterial expression plasmid encoding polycystin-2 ciliary targeting signal with N-terminus GST-tag.

Depositor

Lei Lu

Article

Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393

Insert

Polycystin-2 (Pkd2 Human)

Tags

GST

Expression

Bacterial

Mutation

contains the region from 1-15

Promoter

tac

Available Since

May 26, 2017

Availability

Academic Institutions and Nonprofits only

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CT7-LOVHook-Zdk-HT-GPI

Plasmid

#246600

Purpose

Expresses RudLOV Hook and cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

Halotag7 and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-Zdk-NG-GPI

Plasmid

#246608

Purpose

Expresses RudLOV cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

NeonGreen and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-Zdk-HT-GPI

Plasmid

#246607

Purpose

Expresses RudLOV cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

Halotag7 and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

April 2, 2026

Availability

Academic Institutions and Nonprofits only

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CT7-LOVHook-Zdk-NG-GPI

Plasmid

#246601

Purpose

Expresses RudLOV Hook and cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

NeonGreen and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

April 1, 2026

Availability

Academic Institutions and Nonprofits only

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CT7-LOVHook-Zdk-Sca-GPI

Plasmid

#246602

Purpose

Expresses RudLOV Hook and cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

Scarlet and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

March 25, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-Rho-tag

Plasmid

#249665

Purpose

Expression of rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

Rho-tag

Expression

Bacterial

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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CMV-Zdk-Sca-GPI

Plasmid

#246609

Purpose

Expresses RudLOV cargo(GPI) in mammalian cells.

Depositor

Akiko Satoh

Article

Tago et al Bio Protoc. 2025 Oct 20;15(20):e5468. do

Insert

GPI anchor of CD59 (CD59 Synthetic)

Tags

Scarlet and Zdk1

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2026

Availability

Academic Institutions and Nonprofits only

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pHAGE2-palm-mScar3-10xSunTag

Plasmid

#240626

Purpose

Expresses a fusion construct containing a palmitoylation motif, mScarlet3 and 10 repeats of the SunTag (GCN4) peptide, to luminally tag extracellular vesicles for use in EV-FUSIM assay.

Depositor

Esther Nolte-'t Hoen

Article

van den Ende et al J Extracell Vesicles. 2026 Mar;15(3):e70

Insert

palm-mScarlet3-10xSunTag

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pHalo2X-C1

Plasmid

#251419

Purpose

Mammalian expression vector for expressing a protein of interest with an N-terminal HaloTag

Depositor

Thomas Friedman, Takushi Miyoshi

Article

Miyoshi et al Nat Commun. 2025 Sep 1;16(1):8149. doi:

Type

Empty backbone

Tags

HaloTag

Expression

Mammalian

Mutation

Two silent mustations to disable the XhoI and SalI

Available Since

March 2, 2026

Availability

Academic Institutions and Nonprofits only

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pET-51b(+)_His10x-TEV-SiR-tag

Plasmid

#249666

Purpose

Expression of silicon rhodamine-binding protein tag in bacterial cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

SiR-tag

Expression

Bacterial

Available Since

Feb. 23, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO_CD86-Rho-tag

Plasmid

#249669

Purpose

Expression of monomeric rhodamine-binding protein tag on the cell surface in mammalian cells

Depositor

Kai Johnsson, Richard Wombacher

Article

Kompa et al J Am Chem Soc. 2025 Dec 30. doi: 10.1021

Insert

CD86-Rho-tag

Expression

Mammalian

Available Since

Feb. 3, 2026

Availability

Academic Institutions and Nonprofits only

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pPD684 EnAsCas12a-BFP

Plasmid

#249157

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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pPD686 A2UCOE-EnAsCas12a-BFP

Plasmid

#249159

Purpose

Overexpression of EnAsCas12a-BFP with blasticidin resistance gene. Contains minimal A2UCOE and Cp36 recombination site.

Depositor

Michael Bassik

Article

Arana et al Nat Commun. 2025 Nov 27;16(1):10656. doi

Insert

A2UCOE-EnAsCas12a-TagBFP

Use

CRISPR; Cp36 donor dna

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 7, 2026

Availability

Academic Institutions and Nonprofits only

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