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pLX302-MKP5-V5 puro

Plasmid

#87770

Purpose

Expresses V5 tagged human MAPK phosphatase 5 (MKP5/DUSP10)

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

MKP5 (DUSP10 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

March 22, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB111

Plasmid

#68565

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

3xFLAG

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pJRH-1328 LV EF1a-CD20 IRES-EGFP

Plasmid

#201918

Purpose

2nd generation lentiviral transfer plasmid for engineering cells to express human CD20

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

CD20

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Aug. 9, 2023

Availability

Academic Institutions and Nonprofits only

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pIVT-dCas9-EGFP

Plasmid

#174442

Purpose

in vitro transcription template for dCas9-EGFP

Depositor

Michael Lampson

Article

Kumon et al Cell. 2021 Sep 16;184(19):4904-4918.e11.

Insert

Cas9 Endonuclease Dead

Use

In vitro transcription template

Tags

EGFP

Promoter

T7 SP6

Available Since

Oct. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-hCCND1-HA

Plasmid

#174154

Purpose

Lentiviral vector expressing HA-tagged human cyclin D1

Depositor

Julien Sage

Article

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

CCND1 (CCND1 Human)

Use

Lentiviral

Tags

hemagglutinin (HA)

Expression

Mammalian

Promoter

EF1A

Available Since

Sept. 13, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-PE2-VRQR

Plasmid

#159983

Purpose

Prime editing in mammalian cells

Depositor

Yongsub Kim

Article

Kweon et al Mol Ther. 2021 Feb 23. pii: S1525-0016(2

Insert

PE2-VRQR

Tags

BpSV40 NLS

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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p2T-CMV-eA3Amax-BlastR

Plasmid

#152997

Purpose

C-to-T base editor

Depositor

David Liu

Article

Arbab et al Cell. 2020 Jun 9. pii: S0092-8674(20)306

Insert

eA3A-nSpCas9-UGI-UGI

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available Since

July 1, 2020

Availability

Academic Institutions and Nonprofits only

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pV1326

Plasmid

#111435

Purpose

S. cerevisiae and C. glabrata Solo CRISPR vector, marked with URA3 and NatR

Depositor

Gerald Fink

Article

Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

Insert

CaCas9/sgRNA-BsmBI stuffer

Use

CRISPR

Expression

Yeast

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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pLX_TRC311 ETV4-S

Plasmid

#74983

Purpose

Constitutive ETV4 ORF, blasticidin selection

Depositor

William Hahn

Article

Wang et al Cell Rep. 2017 Feb 7;18(6):1543-1557. do

Insert

ETV4 (ETV4 )

Tags

V5

Expression

Mammalian

Available Since

May 10, 2017

Availability

Academic Institutions and Nonprofits only

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pMpGWB114

Plasmid

#68568

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Glucocorticoid receptor (GR)

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

July 13, 2016

Availability

Academic Institutions and Nonprofits only

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pOT_7-lenti-EFS-SS1-BBz-2A-puro

Plasmid

#217990

Purpose

Expresses anti-Mesothelin CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A for lentiviral delivery.

Depositor

Neville Sanjana

Article

Xue et al bioRxiv [Preprint]. 2024 Nov 27:2024.11.

Insert

SS1-BBz CAR

Use

Lentiviral

Available Since

Nov. 27, 2024

Availability

Academic Institutions and Nonprofits only

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Marathon-RT pentamutant for Split-PE (pEH107)

Plasmid

#190110

Purpose

Expresses Marathon-RT pentamutant (D14R-N26R-D74R-N116K-N197R)

Depositor

Keith Joung

Article

Grunewald et al Nat Biotechnol. 2023 Mar;41(3):337-343.

Insert

Marathon-RT

Tags

bpNLS

Expression

Mammalian

Mutation

D14R, N26R, D74R, N116K, N197R

Promoter

CMV

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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YFVdelCME-mVenus

Plasmid

#179953

Purpose

pCCI vector encoding YFV-17D lacking C, prM, E proteins but with mVenus

Depositor

Wei Xu

Article

Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

Insert

YFV-17D lacking C, prM, E proteins but with mVenus

Use

Transneuronal tracing

Promoter

SP6

Available Since

Feb. 17, 2022

Availability

Academic Institutions and Nonprofits only

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pEXP5-NT/6xHis mCherry F30 2xdBroccoli

Plasmid

#169233

Purpose

Constitutive T7 RNAP-mediated expression of 6xHis-mCherry with 2xd Broccoli RNA aptamer.

Depositor

Dora Tang

Article

Gonzales et al ACS Synth Biol. 2022 Jan 21;11(1):205-21

Insert

6xHis mCherry F30-2xdBroccoli

Expression

Bacterial

Promoter

T7 promoter

Available Since

Dec. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pET28-sfGFP-DogTag Loop A

Plasmid

#171779

Purpose

Expresses in bacterial cytoplasm superfolder GFP with DogTag and flanking linkers between Val22 and Asn23

Depositor

Mark Howarth

Article

Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

Insert

sfGFP-DogTag Loop A

Tags

His6

Expression

Bacterial

Mutation

DogTag and linkers inserted between residues Val2…

Promoter

T7

Available Since

July 29, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-GfaABC1D-GRAB_ATP1.0mut

Plasmid

#167580

Purpose

Express the ATP-insensitive control sensor GRAB_ATP1.0mut in astrocytes

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP-insensitive sensor GRAB_ATP1.0mut (control sensor) (P2RY1 Human)

Use

AAV

Promoter

GfaABC1D

Available Since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA-LoxP-mKate-LoxP-EGFP

Plasmid

#167940

Purpose

CMV promoter-LoxP-mKate-LoxP-EGFP

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

LoxP-mKate-LoxP-EGFP

Expression

Mammalian

Promoter

CMV

Available Since

April 12, 2021

Availability

Academic Institutions and Nonprofits only

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pMS79 (eft-3p::Cas9 + sgRNA)

Plasmid

#154839

Purpose

Cas9 + sgRNA plasmid that is targeted to the synthetic guide sequence GGACAGTCCTGCCGAGGTGG

Depositor

Patrick Phillips

Article

Stevenson et al G3 (Bethesda). 2020 Aug 19. pii: g3.120.

Insert

sgRNA

Use

CRISPR

Expression

Worm

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pKM126

Plasmid

#105134

Purpose

Clostridium difficile CRISPR-cas9 mutagenesis plasmid Tn916 oriT

Depositor

Joseph Sorg

Article

McAllister et al Sci Rep. 2017 Nov 7;7(1):14672. doi: 10.

Inserts

Upstream & Downstream pyrE deletion region
tetR
Cas9
gRNA

Use

E. coli - c. difficile shuttle vector

Available Since

Jan. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pTre-Tight-luciferase-GFP (467)

Plasmid

#65491

Purpose

negative control for expression assays

Depositor

Roderic Guigo, Rory Johnson

Insert

EGFP

Use

Luciferase

Expression

Mammalian

Promoter

tight TRE promoter

Available Since

Sept. 9, 2016

Availability

Academic Institutions and Nonprofits only

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