We narrowed to 7,837 results for: amph
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Plasmid#181950PurposeaTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirKDepositorInsertPhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)
UseTagsmNeonGreenExpressionBacterialMutationPromoterPhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPbuCas13b-gRNA-1
Plasmid#184567PurposeConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.DepositorInsertConstitutive expression of single-spacer CRISPR array with spacer #1 targeting deGFP mRNA for PbuCas13b in bacteria.
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119Available sinceOct. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pSBK.136
Plasmid#187220PurposeExpresses Eco1 ncRNA v35 (long a1/a2) and Eco1 ncRNA v35 (long a1/a2, barcode 6) from promoters pSalTTC and pTet*, respectivelyDepositorInsertRetron Eco1 ncRNA v35 (long a1/a2); Retron Eco1 ncRNA v35 (long a1/a2, barcode 6)
UseTagsExpressionBacterialMutationPromoterA: pSalTTC; B: pTet*Available sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::B1-NLSLacZ-B2
Plasmid#186413PurposeMultisite destination vector for ascidian electroporation of an AttB1/B2 recombined NLSLacZ under control of an AttB3/B5 recombined reg. sequence.DepositorInsertlacZ (lacZ E. coli)
UseGateway destination vectorTagsNuclear localization signalExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmRE-Tn5-142
Plasmid#118531PurposeminiTn5 plasmid to deliver constitutively expressed fluorescent protein genes in bacteriaDepositorInsertmClover3
UseMinitn5 delivery vectorTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pWP001
Plasmid#178596PurposepAF256 derived plasmid encoding a xylose inducible CD16/50L CBD-SmBiT and LgBiTDepositorInsertCBD-SmBiT/LgBiT
UseTagsExpressionBacterialMutationPromoterPxylAvailable sinceApril 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnYC-NvHN-DmXRN1_1323-1355_N
Plasmid#147052PurposeBacterial Expression of DmXRN1_1323-1355DepositorInsertDmXRN1_1323-1355 (pcm Fly)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA1-gltA2
Plasmid#71348PurposegltA1-gltA2 targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA1-gltA2 guide array
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induct…Available sinceJan. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
CDS12_Cas9-NLS
Plasmid#136122PurposePuchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAGDepositorInsertCDS12_AtcoCas9-NLS(noStop)
UseSynthetic BiologyTagsExpressionPlantMutationBsaI/ SapI domesticatedPromoterAvailable sinceNov. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPQD0CM0065
Plasmid#177019PurposeLevel 0 CDS (with stop codon) part for MoClo assembly, AATG-GCTTDepositorInsert1-deoxy-D-xylulose 5-phosphate synthase
UseSynthetic BiologyTagsExpressionMutationtransit peptide removed, amplified from cDNA, con…PromoterAvailable sinceNov. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTF101.1gw3
Plasmid#172181PurposeA binary plasmid for gateway 3-Entry in pTF101.1 background (attR1 & attR2) containing double CaMV 35S promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.DepositorInsertattR3-CmR-ccdB-attR4
UseTagsExpressionPlantMutationPromoterlacUV5 promoterAvailable sinceSept. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
PdnaK-IR2-IR2 GFP pZa
Plasmid#170083PurposeGFP expression under the control of E. coli dnaK promoter engineered with double IR2 HAIR motif from M. tuberculosisDepositorInsertGreen fluorescent protein
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterPdnaK-IR2-IR2Available sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AtU6-26:AsCas12a_DR (GB1442)
Plasmid#160574PurposeAsCas12a direct repeat sequence fused to the A. thaliana U6-26 promoter used for the assembly of gRNA expression cassettes.DepositorInsertAtU6-26:AsCas12a_DR
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 LbCas12a (GB1439)
Plasmid#160573PurposeHuman codon optimized coding sequence of Cas12a from Lachnospiraceae CRISPR system (LbCas12a).DepositorInsertLbCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pUPD2 AsCas12a (GB1438)
Plasmid#160572PurposeHuman codon optimized coding sequence of Cas12a from Acidominococcus sp (AsCas12a).DepositorInsertAsCas12a
UseCRISPR and Synthetic BiologyTagsExpressionMutationBsaI and BsmBI sites removedPromoterAvailable sinceMarch 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc367
Plasmid#161947PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on ECF20DepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-ECF20(6-188)-BsaI_v3
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
IBMc228
Plasmid#161946PurposeContains Type IIS restriction site free elements for IBM staging vectors. Additional BsaI site to facilitate size selection. Staging plasmid for IBM on TetRDepositorInsertpSB1C3(BsmBI-,BsaI+)-BsaI-TetR(20-188)-BsaI
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAT-9251
Plasmid#124226PurposeBacterial SpCas9-HF1 expressionDepositorInsertSpCas9-HF1
UseCRISPRTagsExpressionBacterialMutationPromoterTetR/TetAAvailable sinceFeb. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFJ1820 - pDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
Plasmid#159838PurposeThree-fragment Gateway compatible pDEST vector to clone transgene downstream of smu-1 for germline expression from arraysDepositorInsertpDEST[2-3](5605)[Psmu-1 | smu-1 genomic | ccdB Cm]
UseTagsExpressionWormMutationNot applicablePromoterAvailable sinceNov. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
RA35
Plasmid#141126PurposeExpresses dimeric LacI repressor (LacI-11) under Rhl promoterDepositorInsertsUseTagsssrA degradation tagExpressionBacterialMutationtruncated/dimeric version of LacI (missing last 1…Promoterengineered rhl promoter (responds to C4-HSL) and …Available sinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only