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pEGFP-C1-LgBiT-MYPT1(1-314)(KAKA)-Flag

Plasmid

#224894

Purpose

Mammalian expression of LgBiT-MYPT1(1--314)

Depositor

Mathieu Bollen

Article

Claes et al Cell Chem Biol. 2023 Dec 21;30(12):1666-

Insert

LgBiT-MYPT1(1-314)KAKA-Flag

Expression

Mammalian

Available Since

Nov. 6, 2024

Availability

Academic Institutions and Nonprofits only

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Crimson/P2A-ICP8 (pSLIK1)

Plasmid

#113856

Purpose

For Doxycycline-inducible expression of ICP8 synaptase, with red fluorescent protein gene E2-Crimson-P2A-ICP8

Depositor

Richard S Myers

Article

Valledor et al PLoS One. 2018 Aug 15;13(8):e0200955. do

Insert

ICP8

Use

Lentiviral

Tags

P2A/Crimson

Available Since

June 29, 2022

Availability

Academic Institutions and Nonprofits only

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pLL3.7 Scr shRNA

Plasmid

#59299

Purpose

non-targeting shRNA

Depositor

Ulrike Heberlein

Article

Lasek et al Genes Brain Behav. 2007 Nov;6(8):728-35.

Insert

Scr shRNA

Use

Cre/Lox, Lentiviral, and RNAi

Expression

Mammalian

Promoter

mouse U6

Available Since

Oct. 6, 2014

Availability

Academic Institutions and Nonprofits only
pCGT-Sos Cat

Plasmid

#32923

Depositor

Dafna Bar-Sagi

Article

Boykevisch et al Curr Biol. 2006 Nov 7;16(21):2173-9.

Insert

SOS1 (SOS1 Human)

Tags

T7 epitope

Expression

Mammalian

Mutation

Contains aa 564-1049 (catalytic domain)

Promoter

CMV

Available Since

Jan. 31, 2012

Availability

Academic Institutions and Nonprofits only
scFv-GCN4-sfGFP-deadTET1CD

Plasmid

#184442

Purpose

Catalytically DEAD TET1CD with scFv against GCN4

Depositor

Rhys Allan

Article

Chan et al Nat Commun. 2022 Sep 23;13(1):5582. doi:

Insert

Catalytically DEAD TET1

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Sept. 23, 2022

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2A(sfGFP)

Plasmid

#126964

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

July 31, 2019

Availability

Academic Institutions and Nonprofits only

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pTF101.2

Plasmid

#134771

Purpose

A binary plasmid containing nopaline synthase promoter and TEV enhancer to drive bialophos resistance gene (bar) for plant transformation.

Depositor

Kan Wang

Article

Testroet et al In Vitro Cell.Dev.Biol.-Plant (2017) 53:

Type

Empty backbone

Expression

Plant

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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mSA-phi29

Plasmid

#210815

Purpose

in vitro transcription of mRNA

Depositor

Erik Sontheimer

Article

Liu et al Nat Biotechnol. 2023 Sep 14. doi: 10.103

Insert

mSA-Phi29

Expression

Bacterial

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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M-ST1-sgRNA

Plasmid

#48672

Purpose

Mammalian U6-driven sgRNA (STm1) targeting GTCCCCTCCACCCCACAGTG

Depositor

George Church

Article

Esvelt et al Nat Methods. 2013 Sep 29. doi: 10.1038/n

Insert

sgRNA targeting GTCCCCTCCACCCCACAGTG, compatible with S. thermophilus #1 Cas9, hU6 promoter

Use

CRISPR

Promoter

hU

Available Since

Oct. 22, 2013

Availability

Academic Institutions and Nonprofits only

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pEX128·gRNA

Plasmid

#187600

Purpose

Template for amplification of gRNA with Cas6 recognition site

Depositor

Pablo Ivan Nikel

Article

Volke et al Nat Commun. 2022 May 31;13(1):3026. doi:

Insert

gRNA template

Promoter

no promoter

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pEX-A-U6-gRNA

Plasmid

#65626

Purpose

Empty vector for the expression U6 driven gRNA

Depositor

Sebastian Bultmann

Article

Mulholland et al Nucleic Acids Res. 2015 May 24. pii: gkv

Insert

U6-gRNA

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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aav-tnt-gfp-v2

Plasmid

#165036

Purpose

AAV-based delivery of GFP into cardiomyocytes in vivo

Depositor

William Pu

Article

Guo et al Proc Natl Acad Sci U S A. 2021 Jan 12;11

Type

Empty backbone

Use

AAV

Available Since

March 8, 2021

Availability

Academic Institutions and Nonprofits only

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His SUMO TEV SPOP 1-374

Plasmid

#197015

Purpose

E. coli expression vector

Depositor

Tanja Mittag

Article

Cuneo et al Mol Cell. 2023 Mar 2;83(5):731-745.e4. d

Insert

SPOP (Spop Mouse)

Tags

6His-SUMO-TEV

Expression

Bacterial

Available Since

April 5, 2023

Availability

Academic Institutions and Nonprofits only

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Integrin-αv Headpiece M400GC

Plasmid

#159637

Purpose

Contains integrin alpha v residues 1–594 with the M400C mutation and Gly inserted prior to residue 400

Depositor

Timothy Springer

Article

Dong et al Nat Struct Mol Biol. 2014 Dec;21(12):109

Insert

alpha v headpiece M400GC

Tags

6x His and Strep II

Expression

Mammalian

Mutation

M400C

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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hTRAF2pLPCX-HA-Flag/P874

Plasmid

#20229

Depositor

Martin Dorf

Article

Li et al Mol Cell. 2009 Jan 16. 33(1):30-42.

Insert

TNF receptor-associated factor 2 (TRAF2 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

unmutated NM_021138.3. L replaces M at beginning…

Available Since

March 3, 2009

Availability

Academic Institutions and Nonprofits only

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pEvoltac EcW-TGA-h9

Plasmid

#218765

Purpose

encodes EcWRS-h9 and EcW-tRNA-TGA

Depositor

Abhishek Chatterjee

Article

Italia et al Nat Chem Biol. 2017 Apr;13(4):446-450. d

Insert

EcWRS-h9

Expression

Bacterial

Mutation

S8A, V144S, V146A

Promoter

tacI

Available Since

June 21, 2024

Availability

Academic Institutions and Nonprofits only

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pME-Gal4

Plasmid

#53366

Purpose

full-length native Gal4 with stop in Gateway middle entry clone

Depositor

Joshua Bonkowsky

Article

Fujimoto et al Dev Dyn. 2011 Oct;240(10):2324-34. doi:

Insert

native (full-length) Gal4

Promoter

no promoter

Available Since

May 16, 2014

Availability

Academic Institutions and Nonprofits only

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pLX208-FLiCRE2-TF

Plasmid

#234523

Purpose

Transcriptional reporter for detecting calcium increase in HEK293T (transcription factor)

Depositor

Wenjing Wang

Article

Zhou et al Cell Rep Methods. 2025 Apr 21;5(4):10101

Insert

CD4-HA-CIBN-MK2-hLOV-TEVcs-GAL4

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

April 3, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pRK5_tAKAR4α

Plasmid

#119919

Purpose

Mammalian expression of the tAKAR4α sensor for detecting PKA activity using ratiometric imaging

Depositor

Haining Zhong

Article

Ma et al Neuron. 2018 Aug 22;99(4):665-679.e5. do

Insert

tAKAR4α

Expression

Mammalian

Available Since

March 9, 2019

Availability

Academic Institutions and Nonprofits only

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pFPL-Ch

Plasmid

#61647

Purpose

Gateway vector for generating cyan fluorescent protein fusions in fungi

Depositor

Mark Farman

Article

Gong et al Mol Plant Microbe Interact. 2014 Nov 12.

Insert

enhanced cyan fluorescent protein

Expression

Plant

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pTK_Msx1_enh_264_Citrine

Plasmid

#130610

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

Msx1_enh_264

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pFUGW NLS-Cre

Plasmid

#183425

Purpose

Lentiviral Cre vector

Depositor

Harold MacGillavry

Article

Droogers et al eNeuro. 2022 Jul 18;9(4):ENEURO.0056-22.

Insert

NLS-Cre

Use

Lentiviral

Expression

Mammalian

Available Since

April 25, 2022

Availability

Academic Institutions and Nonprofits only

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pPRIME-TET-GFP-recipient

Plasmid

#11655

Purpose

3rd generation lentiviral transfer vector. pPRIME-mating recipient plasmid with a tetracycline-responsive promoter (TET) controlling GFP expression

Depositor

Stephen Elledge

Article

Stegmeier et al Proc Natl Acad Sci U S A. 2005 Sep 13. 1

Type

Empty backbone

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

March 1, 2007

Availability

Academic Institutions and Nonprofits only

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pJUMP49-2E(sfGFP)

Plasmid

#126995

Purpose

Level 2 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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[pSK600] pRK5 HA-Nprl2(R78A)

Plasmid

#136146

Purpose

mammalian expression of Nprl2

Depositor

David Sabatini, Kuang Shen

Article

Shen et al J Biol Chem. 2019 Feb 22;294(8):2970-297

Insert

Nprl2 (NPRL2 Human)

Tags

HA

Expression

Mammalian

Mutation

R78A

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCN38-ktrA-yfp

Plasmid

#227636

Purpose

c-di-AMP biosensor plasmid. Encodes yfp downstream of c-di-AMP-binding ktrA riboswitch. Plasmid confers ampicillin resistance to E. coli and chloramphenicol resistance to S. aureus.

Depositor

Angelika Grundling

Article

Kviatkovski et al mSphere. 2024 Sep 17:e0032124. doi: 10.1

Insert

ktrA-yfp

Expression

Bacterial

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3-myc3-Tsc1

Plasmid

#19955

Depositor

Yue Xiong

Article

Shumway et al J Biol Chem. 2003 Jan 24. 278(4):2089-92

Insert

Tsc1 (Tuberous sclerosis 1) (TSC1 Human)

Tags

Myc3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only
pET28c-Ku

Plasmid

#161936

Purpose

Expresses 6xHis-tagged Ku from Mycobacterium tuberculosis under T7 promoter for purification

Depositor

Harris Wang

Article

Yim et al ACS Synth Biol. 2020 Sep 29. doi: 10.102

Insert

ku (mku Mycobacterium tuberculosis)

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pJUMP29-1E(sfGFP)

Plasmid

#126982

Purpose

Level 1 vector. OriV 9 (pBBR322/ROP; medium copy number); sfGFP cloning reporter.

Depositor

Chris French

Article

Valenzuela-Ortega et al Synth Biol (Oxf). 2021 Feb 2;6(1):ysab00

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

None

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_TFAP2a_enh_249_Cerulean

Plasmid

#130615

Purpose

enhancer reporter

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

TFAP2a_enh_249

Use

Enhancer reporter

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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