We narrowed to 26,403 results for: Vit

1. D8_Ld_340400

   Plasmid

   #135824

   Purpose

   Expression of Leishmania donovani ectodomain in mammalian cells

   Depositor

   Gavin Wright

   Article

   Roberts et al PLoS Pathog. 2022 Feb 24;18(2):e1010364.

   Insert

   hypothetical protein, unknown function
   

   Tags

   biotinylation sequence and His tag

   Expression

   Mammalian

   Available Since

   Feb. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLenti-FNLSHF1-P2A-Puro

   Plasmid

   #136901

   Purpose

   Lentivirus for constitutive expression of FNLSHF1 in mammalian cells (codon optimized)

   Depositor

   Lukas Dow

   Article

   Katti et al Nucleic Acids Res. 2020 Feb 29. pii: 576

   Insert

   FNLSHF1
   

   Use

   Lentiviral

   Tags

   FLAG-NLS and NLS

   Mutation

   R661A,Q695A,Q926A

   Promoter

   EF1s

   Available Since

   Feb. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pC0.007

   Plasmid

   #119671

   Purpose

   Level 0 part. Promoter, cpc560 promoter, extended spacer region, downstream TFB site region duplicated.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   Pcpc560-Dx2
   

   Use

   Synthetic Biology

   Mutation

   bp 89 T to C, bp 187 C to A, Duplicated downstrea…

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pC0.006

   Plasmid

   #119670

   Purpose

   Level 0 part. Promoter, cpc560 promoter, extended spacer region, upstream TFB site region duplicated.

   Depositor

   Alistair McCormick

   Article

   Vasudevan et al Plant Physiol. 2019 May;180(1):39-55. do

   Insert

   Pcpc560-Ux2
   

   Use

   Synthetic Biology

   Mutation

   bp 89 T to C, bp 187 C to A, Duplicated upstream …

   Available Since

   Aug. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pVR2-m4

   Plasmid

   #84723

   Purpose

   pVR2 but with G to U and A to C mutations following the riboswitch aptamer

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   G to U mutation at the first nucleotide after the…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pVR2-m5

   Plasmid

   #84724

   Purpose

   pVR2 but with an insertion of 1 U immediately following the riboswitch aptamer

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   insertion of 1 U between positions 39 and 40 of a…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pVR2-m6

   Plasmid

   #84725

   Purpose

   pVR2 but with an insertion of 2 U's immediately following the riboswitch aptamer

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   insertion of 2 Us between positions 39 and 40 of …

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pVR2-m7

   Plasmid

   #84726

   Purpose

   pVR2 but with a G to U mutation immediately following the riboswitch aptamer

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   G to U mutation at position 41 of annotated "…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pVR2-m8

   Plasmid

   #84727

   Purpose

   pVR2 but with two G to U mutations immediately following the riboswitch aptamer

   Depositor

   Nils Walter

   Article

   Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

   Inserts

   E. coli RecA promoter
   stretch lacking A's followed by 5' UTR of queC gene
   

   Expression

   Bacterial

   Mutation

   G to U mutations at positions 40 and 41 of annota…

   Available Since

   March 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVR

    Plasmid

    #84718

    Purpose

    E. coli RecA promoter followed by preQ1 riboswitch from the 5' UTR of the queC gene from B. subtilis

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    5' UTR of queC gene
    

    Expression

    Bacterial

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pVR2-m1

    Plasmid

    #84720

    Purpose

    pVR2 but with a C to U mutation in the riboswitch aptamer

    Depositor

    Nils Walter

    Article

    Widom et al Mol Cell. 2018 Nov 1;72(3):541-552.e6. d

    Inserts

    E. coli RecA promoter
    stretch lacking A's followed by 5' UTR of queC gene
    

    Expression

    Bacterial

    Mutation

    C to U mutation at position 13 of annotated "…

    Available Since

    March 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pJB03

    Plasmid

    #111961

    Purpose

    Expresses MBP-GFP-DHFR; construct generated for pull-down and localization experiments

    Depositor

    Matthew Good

    Article

    Caldwell et al Biochemistry. 2018 May 8;57(18):2590-259

    Inserts

    mEGFP
    eDHFR
    

    Tags

    DHFR

    Expression

    Bacterial

    Mutation

    eDHFR;residues 2-159 and enhanced GFP with monome…

    Promoter

    Tac

    Available Since

    Aug. 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28b+R3(R112C/T180C)C

    Plasmid

    #108194

    Purpose

    Expresses PdR-PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28b+3(R112C/T180C)C

    Plasmid

    #108193

    Purpose

    Expresses PCNA3(R112C/T180C)-P450cam fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA3(R112C/T180C)-P450cam fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    R112C/T180C(PCNA3)

    Promoter

    T7 promoter

    Available Since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15b+2(L171C)X

    Plasmid

    #108192

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA2(L171C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET15b+R2(L171C)X

    Plasmid

    #108191

    Purpose

    Expresses PdR-PCNA2(L171C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PdR-PCNA2(L171C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    L171C (PCNA2); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pET15b+1(G108C)X

    Plasmid

    #108189

    Purpose

    Expresses PCNA1(G108C)-PdX fusion protein in E. coli

    Depositor

    Teruyuki Nagamune

    Article

    Haga et al Biotechnol J. 2018 Dec;13(12):e1800088.

    Insert

    PCNA1(G108C)-PdX fusion protein
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    G108C (PCNA1); C73S/C85S (PdX)

    Promoter

    T7 promoter

    Available Since

    April 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKDsgRNA-fsaA

    Plasmid

    #102286

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting fsaA.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    fsaA gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKDsgRNA-adhE

    Plasmid

    #102290

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting adhE.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    adhE gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pKDsgRNA-ompT

    Plasmid

    #102291

    Purpose

    Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting ompT.

    Depositor

    Michael Lynch

    Article

    Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

    Insert

    ompT gRNA
    

    Use

    CRISPR

    Expression

    Bacterial

    Promoter

    pTet

    Available Since

    April 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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