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Plasmid#105670PurposeMammalian expression construct of mouse SIRT1 full length isoformDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSIRT1 (Sirt1 Mouse)
ExpressionMammalianMutationSynonymous base changes not affecting the protein…PromoterCMVAvailable sinceApril 6, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
VPR-ps-dSpCas9
Plasmid#102855PurposeA single-chain light-controllable dSpCas9 with pdDronpa1.2 domainsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertdSpCas9, pdDronpa1.2
UseCRISPR and Synthetic BiologyTagsNLS and VP64-p64-Rta transcription activatorExpressionMammalianPromoterPGKAvailable sinceNov. 27, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMH-SFB-RNF8
Plasmid#99396PurposeMammalian expression construct for S protein-Flag-Streptavidin binding peptide (SFB)-tagged RNF8DepositorInsertRNF8 (RNF8 Human)
TagsSFB (S protein-FLAG-Streptavidin binding peptide)ExpressionMammalianAvailable sinceAug. 14, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDONR223_MAX_WT
Plasmid#82888PurposeGateway Donor vector containing MAX, part of the Target Accelerator Plasmid Collection.DepositorAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST26-C-FLAG
Plasmid#79275PurposeDestination vector containing a C-terminal FLAG tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertFLAG
ExpressionMammalianPromoterCMVAvailable sinceMarch 13, 2017AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pTlpA36-Wasabi
Plasmid#86115PurposemWasabi reporter gene gated by TlpA36DepositorInsertsTlpA36
mWasabi
Tags6xHisExpressionBacterialMutationD341A, P60L, D135V, K187R, K202I, L208QPromoterpTlpA and pTlpA, LacIAvailable sinceFeb. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-PLAA
Plasmid#85669PurposeExpression of PLAA with N-terminal GFP tagDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPLAA (PLAA Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceJan. 31, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSLIK CA MLCK
Plasmid#84647PurposeLentiviral expression of doxycycline-inducible constitutively active MLCK and co-expression of VenusDepositorAvailable sinceDec. 9, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pWalium20-10XUAS-3XFLAG-dCas9-VPR
Plasmid#78897PurposeExpresses 3XFLAG-dCas9-VPR (human codon) under 10XUAS control, for in vivo CRISPRaDepositorInsertdCas9-VPR
Tags3xFLAGExpressionInsectPromoter10XUASAvailable sinceOct. 11, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
TriEx-4 DHX36
Plasmid#68368PurposeExpresses the N-terminal histidine-tagged DXH36 gene product upon transfection into Rosetta 2 E. coli and induction with IPTG.DepositorInsertDHX36 (DHX36 Human)
TagsHistidineExpressionBacterial, Insect, and Mamm…PromoterCMV ie enhancer/promoterAvailable sinceSept. 29, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSRK2721
Plasmid#78572PurposeGateway destination vector for production of MBP-3C site-His6-TEV site-passenger protein fusionsDepositorInsertGateway cloning cassette
ExpressionBacterialAvailable sinceSept. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
FUGW-d2GFP-200
Plasmid#79602PurposeFUGW lentiviral vector with constiutively expressed destabilized GFP (d2GFP) regulated by 5 miR-200 family binding sitesDepositorPublicationInsertd2GFP + miR-200 family targets
UseLentiviralAvailable sinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
PCS4 3XFLAG-PPARgamma1
Plasmid#78769PurposeTo overexpress PPARgamma1 in Mammalian CellsDepositorAvailable sinceJune 10, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR)
Plasmid#71485PurposeBase plasmid for sgRNA cloning with Hygromycin resistance and Tol2 transposon armsDepositorTypeEmpty backbonePromoterU6Available sinceJan. 7, 2016AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHBV-S2-Luc
Plasmid#71417PurposeTo see HBV S2 promoter activity for luciferase assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV S2 promoter driving luciferase expression
UseLuciferaseExpressionMammalianPromoterHBV S2Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHBV-S1-Luc
Plasmid#71416PurposeTo see S1 promoter activity of HBV for luciferase assayDepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHBV S1 promoter driving Luciferase expression
UseLuciferaseExpressionMammalianPromoterHBV S1Available sinceDec. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCAG-C-CreintG
Plasmid#69573PurposeOne half component of the optimized Cre recombinase dependent on GFP (CRE-DOG OPT) for expression in mammalian cellsDepositorInsertkozak consensus - C-CreintG
UseSynthetic BiologyExpressionMammalianPromoterCAGAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMpGWB337
Plasmid#68061PurposeGateway-based vector for Marchantia polymorpha to express a floxed gene, with an expression cassette for Cre-glucocorticoid receptor fusion protein under the control of heat-inducible promoterDepositorTypeEmpty backboneUseCre/LoxExpressionPlantPromoterMpEF1alpha promoterAvailable sinceSept. 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pLJSRSF7
Plasmid#64693PurposeHis-MBP-Smt3-NpuDnaEN102-CBDDepositorTypeEmpty backboneTagsCBD, HisTag, MBP, NpuDnaE-N102, and Smt3ExpressionBacterialPromoterT7Available sinceSept. 11, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Nxph4-2A-CreERT2 Targeting Vector
Plasmid#62219PurposeTargets CreERT2 to the mouse Nxph4 stop codonDepositorInsertNxph4-2A-CreERT2 (Nxph4 Bacteriophage, Mouse)
UseCre/Lox and Mouse TargetingExpressionMammalianPromoterMouse Nxph4Available sinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only