We narrowed to 30,479 results for: des.2

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pRSF-KpDcr1deltaC(GiVL-1)

Plasmid

#32032

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Mutation

H123-N143 replaced by P642-Y645 of GiDicer

Promoter

T7

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1(1-384)

Plasmid

#32031

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(1-384)

Tags

His6-SUMO

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2011

Availability

Academic Institutions and Nonprofits only

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pRSF-KpDcr1deltaC(E147Q/D151N)

Plasmid

#32024

Depositor

David Bartel

Article

Weinberg et al Cell. 2011 Jul 22;146(2):262-76.

Insert

DCR1(15-355)

Tags

His6-SUMO

Expression

Bacterial

Mutation

E147Q/D151N

Promoter

T7

Available Since

Sept. 30, 2011

Availability

Academic Institutions and Nonprofits only

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bactin:mKate2-ras

Plasmid

#105963

Purpose

transgenesis, ubiquitous expression

Depositor

Caren Norden

Article

Icha et al J Cell Biol. 2016 Oct 24;215(2):259-275.

Insert

Ras (KRAS Human)

Tags

mKate2

Expression

Bacterial

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV_ACTB CRISPIE

Plasmid

#172853

Purpose

Actb sgRNA & DRS-2 sgRNA expression under U6. CRISPIE donor mEGFP phase (0-0), excised by DRS-1 or DRS-2 (with SpCas9). Also expresses mRuby3 under the CBA promotor. See Zhong et al, eLife 2021.

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Inserts

Actb intron 1 sgRNA
DRS-2 sgRNA
CRISPIE designer exon (phase 0-0) encoding mEGFP
mRuby3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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SMC2-mAC donor (Hygro)

Plasmid

#140648

Purpose

SMC2 tagging with mAID-Clover

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

SMC2 (SMC2 Human)

Use

CRISPR

Expression

Mammalian

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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AbVec2.1-mIglc2

Plasmid

#127156

Purpose

Expression of immunoglobulin light chains in mammalian cells, mouse (C57BL/6), lambda 2 constant region

Depositor

Hedda Wardemann

Article

Lorenzo et al Nature. 2021 Jan;589(7841):287-292. doi:

Insert

Iglc2 (Iglc2 Mouse)

Expression

Mammalian

Promoter

hCMV IE1 gene promoter

Available Since

Nov. 20, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo G-Linker nsp1

Plasmid

#175426

Purpose

Expresses G-linker CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

G-linker nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

G-linker in place of the RNase destabilization do…

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pEMS1424

Plasmid

#29220

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-EGFP-NLS

Use

High copy number homologous recombination plasmid…

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

Nov. 16, 2011

Availability

Academic Institutions and Nonprofits only

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pEMS1626

Plasmid

#29281

Purpose

The plasmid contains the Pleaides (Ple) MiniPromoter Ple155 derived from the human PCP2 gene and designed for expression in ON Bipolar cells of the retina.

Depositor

Elizabeth Simpson

Article

Korecki et al Gene Ther. 2021 Feb 2. pii: 10.1038/s414

Insert

Ple155-Intron-LacZ-NLS

Use

High copy number homologous recombination plasmid…

Expression

Mammalian

Promoter

MiniPromoter Ple155 from the human PCP2 gene

Available Since

June 10, 2011

Availability

Industry, Academic Institutions, and Nonprofits

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pLenti6.3delta4 GPR3-V5

Plasmid

#172601

Purpose

Lentiviral plasmid expressing c-terminal V5-tagged GPR3

Depositor

Robert Screaton

Article

Iorio et al Commun Biol. 2021 Jul 23;4(1):907. doi:

Insert

G protein-Coupled Receptor 3 (GPR3 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Aug. 12, 2021

Availability

Academic Institutions and Nonprofits only

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EGFP-hPCM2w/b delta6

Plasmid

#47588

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2w/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only

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EGFP-hPMCA2x/b delta6

Plasmid

#47587

Depositor

Emanuel Strehler

Article

Chicka et al J Biol Chem. 2003 May 16;278(20):18464-7

Insert

PMCA2x/b delta6 (ATP2B2 Human)

Tags

EGFP-HA-His

Expression

Mammalian

Mutation

6 C-terminal AA deleted (needed for PDZ binding)

Promoter

CMV

Available Since

Oct. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA4TO-3XFLAG Halo R124A/K125A nsp1

Plasmid

#175417

Purpose

Expresses R124A/K125A CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 R124A/K125A nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4 3xFLAG Halo delta 122-130 nsp1

Plasmid

#175425

Purpose

Expresses delta 122-130 CoV2 nsp1 in mammalian cells. This contains a 3XFLAG Halo tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV2 delta 122-130 nsp1

Tags

3XFLAG Halo

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 8, 2021

Availability

Academic Institutions and Nonprofits only

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Ub-GyrA

Plasmid

#128737

Purpose

Expresses Ub fused to GyrA intein at the C-terminal

Depositor

Sharon Rozovsky

Article

Liu et al Chembiochem. 2019 Jul 2. doi: 10.1002/cb

Insert

UBC

Tags

Mxe GyrA

Expression

Bacterial

Promoter

T7

Available Since

Sept. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pbacPuroR

Plasmid

#26348

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::PuroR::bacterial 3p flank

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pbacNeoR

Plasmid

#26349

Depositor

Ben Lehner

Article

Semple et al Nat Methods. 2012 Jan 30;9(2):118-9. doi

Insert

Bacterial promoter::NeoR::bacterial 3p flank

Expression

Bacterial

Available Since

Dec. 3, 2010

Availability

Academic Institutions and Nonprofits only

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pHM066

Plasmid

#225524

Purpose

Encodes Wnt-responsive destablized iRFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Insert

iRFP

Expression

Mammalian

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pHM126

Plasmid

#225528

Purpose

Encodes Wnt-responsive destablized GFP

Depositor

Jared Toettcher

Article

McNamara et al Nat Cell Biol. 2024 Oct 2. doi: 10.1038/

Insert

GFP

Expression

Mammalian

Available Since

Nov. 20, 2024

Availability

Academic Institutions and Nonprofits only

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