We narrowed to 8,596 results for: Set

1. pMDC123SB-OsMIR390-B/c

   Plasmid

   #61466

   Purpose

   Plant expression vector (2x35S) for direct cloning of artificial microRNAs into rice MIR390 precursor. To use for expressing amiRNAs in monocots.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Expression

   Plant

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Promoter

   2x35S

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCXLE-dCas9VPP300-T2A-GFP-shP53

   Plasmid

   #102896

   Purpose

   EBNA episome plasmid for CAG promoter constitutive expression of dCas9-VP192-P300core followed by T2A-GFP as a reporter. Includes p53 shRNA expression cassette.

   Depositor

   Timo Otonkoski

   Article

   Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

   Insert

   dCas9VPP300-T2A-GFP-shP53
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Promoter

   CAG

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA4/TO CD16.7-h-PKD1 FLM R4114A

   Plasmid

   #83461

   Purpose

   Cytoplasmic tail of human PC1 expression cassette with a membrane anchor and a mutation in the first Calmodulin binding domain (503)

   Depositor

   Thomas Weimbs

   Article

   Doerr et al PLoS One. 2016 Aug 25;11(8):e0161525. do

   Insert

   Polycystin-1 (PKD1 Human)
   

   Tags

   CD16.7

   Expression

   Mammalian

   Mutation

   Amino-acids 4078-4303, R4113A

   Available Since

   Nov. 3, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. AID-BFP-loxP_myo2_neoR

   Plasmid

   #194054

   Purpose

   Dual-selection cassette plasmid for knocking-in AID-BFP into the C. elegans genome

   Depositor

   Kota Mizumoto

   Article

   Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

   Insert

   IAA17 (AXR3 Mustard Weed)
   

   Tags

   bfp

   Expression

   Worm

   Promoter

   None

   Available Since

   Jan. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. Direct-seq_lentiGuide-Puro-Tail-8A8G

   Plasmid

   #157981

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. Sox17(2A-phiC31o)

   Plasmid

   #140762

   Purpose

   Targeting vector to Sox17 gene locus for induction of P2A-fused phiC31o, with PGK-EM7-puroR drug resistant cassette flanked by FRT(F5) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   phiC31o
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox17

   Available Since

   July 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ins:BB1.0L; cryaa:RFP

   Plasmid

   #110283

   Purpose

   A zebrafish insulin promoter driving the Brainbow (BB1.0L) cassette. Contains red eye marker, and is flanked with I-SceI sites.

   Depositor

   Nikolay Ninov

   Article

   Singh et al Nat Commun. 2017 Sep 22;8(1):664. doi: 1

   Insert

   Brainbow1.0L
   

   Use

   Zebrafish

   Promoter

   insulin

   Available Since

   May 31, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-OsMIR390-B/c

   Plasmid

   #61468

   Purpose

   Gateway-compatible entry vector for direct cloning of artificial microRNAs into rice MIR390 precursor.

   Depositor

   James Carrington

   Article

   Carbonell et al Plant J. 2015 Mar 24. doi: 10.1111/tpj.1

   Insert

   OsMIR390-B/c
   

   Use

   Gateway entry vector

   Mutation

   O. sativa MIR390 precursor sequence including a c…

   Available Since

   March 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. Sox1(2A-Cre)

   Plasmid

   #140760

   Purpose

   Targeting vector to Sox1 gene locus for induction of P2A-fused Cre, with PGK-EM7-puroR drug resistant cassette flanked by FRT(WT) sequence.

   Depositor

   Hideyuki Okano

   Article

   Serizawa et al Development. 2019 Nov 4;146(21). pii: de

   Insert

   Cre
   

   Use

   Mouse Targeting

   Promoter

   Endogenous Sox1

   Available Since

   June 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX4

    Plasmid

    #183903

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti PUb

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX3

    Plasmid

    #183904

    Purpose

    donor plasmid for CRISPR Cas9 knockin near the Aedes aegypti m / M locus

    Depositor

    Eric Marois

    Article

    Lutrat et al Commun Biol. 2023 Jun 16;6(1):646. doi:

    Insert

    GFP
    

    Expression

    Insect

    Promoter

    Aedes aegypti Polyubiquitin

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSBK.009

    Plasmid

    #187209

    Purpose

    Expresses retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG]), and Cas1 + Cas2

    Depositor

    Seth Shipman

    Article

    Bhattarai-Kline et al Nature. 2022 Aug;608(7921):217-225. doi:

    Inserts

    Retron Eco1 ncRNA v35 (long a1/a2, barcode 1 [CCT-AGG])
    Cas1 + Cas2
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Promoter

    T7/Lac

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAS_4xhyb PylT sfGFP 150TAG

    Plasmid

    #154771

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid ) and amber suppression reporter sfGFP 150 TAG stop, for transient transfection or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    sfGFP
    

    Expression

    Mammalian

    Mutation

    150TAG

    Promoter

    EF1

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pOCC94

    Plasmid

    #118912

    Purpose

    shuttle vector for baculovirus production, using FlashBac bacmid; for recombinant protein with honey bee melitine secretion signal, N-terminal MBP, cleavable with 3C protease, and C-terminal eGFP-HIS6

    Depositor

    Marc Bickle, PEPC MPI CBG

    Article

    Lemaitre et al BMC Biotechnol. 2019 Mar 29;19(1):20. do

    Insert

    NcoI-HBMss-MBP-3C-NotI-ccdB-AscI-eGFP-HIS6-stop-HindIII cassette
    

    Tags

    MBP, cleavable with 3C protease and eGFP-HIS6

    Expression

    Insect

    Promoter

    polH

    Available Since

    Jan. 2, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pNAS1b split mCherry NEDD4-2 WW2

    Plasmid

    #111883

    Purpose

    Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

    Depositor

    Jesse Rinehart

    Article

    Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

    Type

    Empty backbone

    Tags

    Split mCherry system: NEDD4-2 WW2 fused to C-term…

    Expression

    Bacterial

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pGfa28-nLac

    Plasmid

    #53130

    Purpose

    Expression of LacZ driven by partial human gfa2 promoter, expression is localized to a subset of astrocytes in mice.

    Depositor

    Michael Brenner

    Article

    Lee et al Glia. 2006 May;53(7):677-87.

    Insert

    Gfa2 (GFAP Human)
    

    Use

    Mouse Targeting

    Tags

    LacZ, NLS, and mP1

    Expression

    Mammalian

    Mutation

    contains the A and B regions (bp -1757 to -1489) …

    Promoter

    human Gfa2

    Available Since

    July 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pBBK92 pHomL-TDH3p-Scarlet(rfp)-Link-SCS2tmh-SSA1t-HomR (AmpR)

    Plasmid

    #179076

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-SCS2tmh, an overexpressed (bright) marker for the endoplasmic reticulum

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Scarlet(rfp)-Link-SCS2tmh
    

    Expression

    Yeast

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pBBK89 pHomL-TDH3p-Scarlet(rfp)-Link-2xPH-SSA1t-HomR (AmpR)

    Plasmid

    #179073

    Purpose

    Vector containing a yeast integration cassette for expressing TDH3p-Scarlet(rfp)-Link-2xPH, an overexpressed (bright) marker for the plasma membrane

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    TDH3p-Scarlet(rfp)-Link-2xPH
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBBK87 pHomL-RPL18Bp-TOM70-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179071

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of TOM70-Link-Scarlet(rfp), a marker for the mitochondria

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-TOM70-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBBK85 pHomL-RPL18Bp-PEX25-Link-Scarlet(rfp)-SSA1t-HomR (AmpR)

    Plasmid

    #179069

    Purpose

    Vector containing a yeast integration cassette for RPL18B-driven expression of PEX25-Link-Scarlet(rfp), a marker for the peroxisome

    Depositor

    Vincent Martin

    Article

    Bean et al G3 (Bethesda). 2022 Jun 16. pii: 6609175

    Insert

    RPL18Bp-PEX25-Link-Scarlet(rfp)
    

    Expression

    Yeast

    Available Since

    May 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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