We narrowed to 13,384 results for: car

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pCDNA3-SREBP-2-GFP (A1134-1135)

Plasmid

#204203

Purpose

Mammalian expression of SREBP-2

Depositor

Allan Weissman

Article

Yang et al J Biol Chem. 2023 Oct 12;299(12):105346.

Insert

SREBP-2 (SREBF2 Human)

Tags

T7x3 and eGFP

Expression

Mammalian

Mutation

Gly to Ala 1134, 1135

Available Since

April 8, 2024

Availability

Academic Institutions and Nonprofits only

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mApple-1xHp

Plasmid

#214409

Purpose

Lipid droplet and ER localization of 1xHp of Spastin fused to mApple

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

Spastin aa 43-92 (SPAST Human)

Tags

mApple

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA7.10-SpG N aa2-713-InteinN

Plasmid

#206967

Purpose

Expresses TadA7.10 and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA7.10, SpG N

Use

AAV

Promoter

CMV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-TadA8e-SpG N aa2-713-InteinN

Plasmid

#206968

Purpose

Expresses TadA8e and SpG cas9N by the constitutive CMV promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

CMV, TadA8e, SpG N

Use

AAV

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-CASI-InteinC-NG C aa714-1368-U6-Lmna sgRNA1

Plasmid

#206974

Purpose

Expresses NG cas9C by the constitutive CASI promoter and sgRNA targeting murine Lmna c.1621T mutation by U6 promoter

Depositor

Yuxuan Guo

Article

Yang et al J Cell Mol Med. 2024 Feb;28(4):e18145. d

Insert

NG aa714-1368, U6, Lmna sgRNA1

Use

AAV

Promoter

U6

Available Since

Feb. 13, 2024

Availability

Academic Institutions and Nonprofits only

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GL4.10 E2E1- Prg4 promoter-luciferase

Plasmid

#195035

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1 and Enhancer 2 (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E3E2E1-Prg4 promoter-luciferase

Plasmid

#195036

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

Prg4 promoter plus Enhancer 1, Enhancer 2, and Enhancer 3 (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pGL4.10 E4E3E2E1-Prg4 promoter-luciferase

Plasmid

#195037

Purpose

Prg4 enhancer/promoterfirefly luciferase constructs were constructed by cloning various Prg4 regulatory fragments into pGL4.10[luc2] vector

Depositor

Andrew Lassar, Cheng-Hai Zhang

Article

Zhang et al Commun Biol. 2021 Mar 12;4(1):332. doi:

Insert

pGL4.10 E4E3E2E1-Prg4 promoter-luciferase (PRG4 Bovine)

Use

Luciferase

Available Since

Feb. 8, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgLMNA

Plasmid

#170546

Purpose

Expresses a sgRNA for 5' tagging to LMNA and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of LMNA

Use

CRISPR and Lentiviral

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pLV.5’eGFP/sgVIM

Plasmid

#170548

Purpose

Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP without homology arms

Depositor

Jacob Giehm Mikkelsen

Article

Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

Insert

sgRNA targeting 5'- end of VIM

Use

CRISPR and Lentiviral

Promoter

U6

Available Since

Jan. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pET30b jGCaMP8f L27A

Plasmid

#204138

Purpose

Production of recombinant calcium sensor jGCaMP8f L27A fast decay variant in E.coli

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Bacterial

Mutation

Leucine 27 changed to Alanine

Promoter

T7

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pGP-CMV-jGCaMP8f L27A

Plasmid

#204140

Purpose

Fluorescent reporter for calcium imaging in mammalian cells - jGCaMP8f with L27A mutation

Depositor

Katalin Torok

Article

Tran et al Front Cell Neurosci. 2023 May 18;17:1155

Insert

jGCaMP8f L27A

Tags

N-terminal His tag

Expression

Mammalian

Mutation

Leucine 27 changed to Alanine

Promoter

CMV

Available Since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR4 genomic A. thaliana PLDGAMMA3

Plasmid

#209188

Purpose

pENTR4 plasmid with genomic sequence of Arabidopsis thaliana PHOSPHOLIPASE D GAMMA 3 (PLDGAMMA3, AT4G11840) without stop codon for C-terminal epitope tags

Depositor

Lennart Wirthmueller

Article

Simranjit et al bioRxiv 2023.09.25.558804

Insert

AT4G11840 (PLDGAMMA3 Mustard Weed)

Use

Gateway-compatible entry vector

Promoter

none

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UUA)_EF1_sfGFP150TAA

Plasmid

#174897

Purpose

ochre suppression reporter sfGFP150TAA expression, with MmaPylT(UUA) ochre suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sf GFP

Expression

Mammalian

Mutation

150TAA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pAS_4xMmaPylT(UCA)_EF1_sfGFP150TGA

Plasmid

#174898

Purpose

opal suppression reporter sfGFP150TGA expression, with MmaPylT(UCA) opal suppressor tRNA cassette

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

sfGFP

Expression

Mammalian

Mutation

150TGA in sfGFP

Promoter

EF1

Available Since

Nov. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT020-LAT_dCore-HaloTag-3x FLAG

Plasmid

#202504

Purpose

Express dCore Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

Truncated transmembrane domain

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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pJPT019-LAT_C26A-HaloTag-3x FLAG

Plasmid

#202503

Purpose

Express C26A Lat mutant

Depositor

Neha Kamat

Article

Peruzzi et al Nat Commun. 2024 Jul 4;15(1):5618. doi:

Insert

LAT (LAT Human)

Tags

HaloTag-3x FLAG

Expression

Mammalian

Mutation

C26A

Promoter

CMV

Available Since

June 26, 2023

Availability

Academic Institutions and Nonprofits only

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Aga2p-LOV-Turbo1-myc_pCTCON2

Plasmid

#199673

Purpose

expresses pre-evolved version of LOV-Turbo on the yeast surface

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

LOV-Turbo1

Tags

Aga2p and Myc

Expression

Yeast

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV_CaMKIIa_SERT_mCherry

Plasmid

#198736

Purpose

Serotonin transporter with mCherry fluorescent protein driven by CaMKIIa promoter on a AAV vector

Depositor

Lin Tian

Article

Vargas et al Science. 2023 Feb 17;379(6633):700-706.

Insert

Serotonin transporter and mCherry

Use

AAV

Expression

Mammalian

Promoter

CaMKIIa

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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KTK_005

Plasmid

#180555

Purpose

Entry vector containing Terminator sequence (Bba_B0015)

Depositor

Tom Ellis

Article

Goosens et al ACS Synth Biol. 2021 Dec 17;10(12):3422-

Insert

Terminator sequence (Bba_B0015)

Expression

Bacterial

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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