We narrowed to 14,251 results for: TIM

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pBDP

Plasmid

#217827

Purpose

Stuffer plasmid with golden gate compatible bidirectional promoter

Depositor

Timothy Whitehead

Article

Petersen et al Nat Commun. 2024 May 10;15(1):3974. doi:

Insert

Gal1/Gal10 Bidirectional Promoter

Use

Cloning vector

Available Since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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1BR9-AtWUS

Plasmid

#193865

Purpose

E. coli expression vector for N-ter GFP11 and C-ter R9 tagged WUSCHEL protein from Arabidopsis thaliana.

Depositor

Markita Landry

Article

Wang et al Commun Biol. 2023 Aug 12;6(1):840. doi:

Insert

AtWUS E. Coli Codon Optimized

Tags

6xHis, GFP11, and R9

Expression

Bacterial

Promoter

T7 Promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-MBP-DQNAT

Plasmid

#198215

Purpose

pJL1 plasmid encoding E. coli maltose binding protein (MBP) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

MBP_DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pJL1-PD-DQNAT

Plasmid

#198216

Purpose

pJL1 plasmid encoding H. influenzae protein D (PD) modified at the C-terminus with 30 amino acids containing one optimal DQNAT sequon followed by a 6x-His tag

Depositor

Michael Jewett

Article

Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:

Insert

PD-DQNAT

Tags

6x His Tag and DQNAT glycosylation tag

Expression

Bacterial

Promoter

T7

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pcoCASphi_E9t_V2_CmYLCVp_35sT_ribozyme_AtPDS3_gRNA10

Plasmid

#197958

Purpose

T-DNA binary vector to express pcoCasphi driven by UBQ10 gene promoter and a single AtPDS3 gRNA driven by CmYLCVp and flanked by ribozymes.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pcoCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

CmYLCVp and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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pC1300_pUB10_pco-vCASphi_E9t_V2_U6_AtPDS3_gRNA10

Plasmid

#197963

Purpose

T-DNA binary vector to express pco-vCasphi driven by UBQ10 gene promoter, and the AtPDS3 guide RNA 10 driven by U6 promoter.

Depositor

Steven Jacobsen

Article

Li et al Proc Natl Acad Sci U S A. 2023 Jan 24;12

Inserts

pco-vCasphi-2
AtPDS3 gRNA10

Use

CRISPR

Expression

Plant

Promoter

AtU6-26 and pUBQ10

Available Since

March 30, 2023

Availability

Academic Institutions and Nonprofits only

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GB1-His-Tev-ALIX-V (348-702)

Plasmid

#189819

Purpose

Codon-optimized bacterial expression plasmid. Expresses GB1-His-Tev-ALIX-V (348-702).

Depositor

Lalit Deshmukh

Article

Nelson et al J Am Chem Soc. 2023 Apr 3. doi: 10.1021/

Insert

GB1-His-Tev-ALIX-V (348-702) (PDCD6IP Human)

Tags

GB1-His-Tev

Expression

Bacterial

Available Since

March 23, 2023

Availability

Academic Institutions and Nonprofits only

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FUiLMO2W

Plasmid

#129480

Purpose

3rd-generation lentiviral vector for iLMO2, an opto-chemogenetic probe for neuronal inhibition by light or chemical

Depositor

Robert Gross

Article

Tung et al Sci Rep. 2015 Sep 24;5:14366. doi: 10.10

Insert

iLMO2

Use

Lentiviral

Expression

Mammalian

Promoter

human ubiquitin C

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160a

Plasmid

#173756

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160a_sgRNA2/R160a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R160b

Plasmid

#173757

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA160b

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/R160b_sgRNA2/160b

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDe_Cas9_KANA_R390a

Plasmid

#173758

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and sgRNA for miRNA390a

Depositor

Kristina Gruden

Article

Lukan et al Hortic Res. 2022 Jun 30;9:uhac147. doi:

Insert

Cas9_sgRNA1/390a_sgRNA2/390a

Use

CRISPR

Promoter

AtU6 &StU6

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pSCL.037

Plasmid

#184966

Purpose

Express -Eco1 dead RT and ncRNA in yeasst

Depositor

Seth Shipman

Article

Lopez et al Nat Chem Biol. 2022 Feb;18(2):199-206. d

Insert

Eco1: RT and ncRNA(wt), a1/a2 length: 12, dead RT

Expression

Yeast

Mutation

Human codon optimized RT, YXDD catalytic region m…

Promoter

Gal7

Available Since

Nov. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV492

Plasmid

#177700

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RHR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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pNB101

Plasmid

#169110

Purpose

Feedback-controlled AraC circuit

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

araC (araC Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB200

Plasmid

#169111

Purpose

TESEC backbone for target expression

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

lacZ (lacZ Escherichia coli K12 MG1655)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB201

Plasmid

#169112

Purpose

TESEC expression vector for Alr from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

alr (alr Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB203

Plasmid

#169114

Purpose

TESEC expression vector for mEGFP-Alr fusion construct

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

mEGFP-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB204

Plasmid

#169115

Purpose

TESEC expression vector for 6xHis-Alr fusion constrcut

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

6xHis-alr (alr Synthetic, Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Tags

6xHis

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB205

Plasmid

#169116

Purpose

TESEC expression vector for Asd from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

asd (asd Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB206

Plasmid

#169117

Purpose

TESEC expression vector for CysH from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

cysH (cysH Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB207

Plasmid

#169118

Purpose

TESEC expression vector for DapB from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

dapB (dapB Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pNB208

Plasmid

#169119

Purpose

TESEC expression vector for TrpD from M. tuberculosis

Depositor

Edwin Wintermute

Article

Bongaerts et al Nat Commun. 2022 Jul 7;13(1):3905. doi:

Insert

trpD (trpD Mycobacterium tuberculosis H37Rv)

Use

Synthetic Biology

Expression

Bacterial

Mutation

Codon optimized for E. coli

Promoter

pBAD

Available Since

July 8, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV491

Plasmid

#177699

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species.

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

RNR2p (Debaryomyces hansenii ) and TEF1p (Ashbya …

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pDIV488

Plasmid

#177696

Purpose

Plasmid expressing optimized Cas9 and NAT marker. Compatible with CTG-clade yeast species

Depositor

Uffe Mortensen

Article

Strucko et al Synth Biol (Oxf). 2021 Oct 12;6(1):ysab0

Inserts

Cas9
Nat

Use

CRISPR

Tags

SV40

Expression

Bacterial and Yeast

Promoter

TDH3p (Candida lusitaniae) and TEF1p (Ashbya goss…

Available Since

March 11, 2022

Availability

Academic Institutions and Nonprofits only

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pLN492 (FuGW-G8p-antiPD1(HC)-1Pe)

Plasmid

#105194

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab heavy chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(HC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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pLN493 (FuGW-G8p-antiPD1(LC)-1Pe)

Plasmid

#105195

Purpose

Module 3 - GAL4 synthetic promoter (with eight binding sites) drives the expression of anti-PD1 Ab light chain transcripts containing miR1-Bs(Pe)

Depositor

Timothy Lu

Article

Nissim et al Cell. 2017 Nov 16;171(5):1138-1150.e15.

Insert

G8p-antiPD1(LC)-1Pe

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available Since

Feb. 10, 2022

Availability

Academic Institutions and Nonprofits only

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CDS_Cas9-NLS

Plasmid

#136121

Purpose

Puchta Arabidopsis codon-optimised Cas9 for CRISPR

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

CDS_AtcoCas9-NLS

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-gp130dimer-stm2585

Plasmid

#174395

Purpose

Constitutively active gp130 dimer with codon-optimized stm2585 insert (replacing amino acids 140-179)

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

IL6ST cytosolic domain and sarA chimera

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Flag-stm2585

Plasmid

#174391

Purpose

S. Typhimurium gene stm2585 (sarA/steE) codon-optimized for mammalian expression with N-terminal Flag tag

Depositor

Dennis Ko

Article

Gibbs et al Cell Host Microbe. 2020 Jan 8;27(1):129-

Insert

stm2585

Tags

Flag

Expression

Mammalian

Available Since

Sept. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pJS697

Plasmid

#168778

Purpose

Yeast surface display plasmid backbone that can be combined with pJS699 through homologous recombination

Depositor

Timothy Whitehead

Article

Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

Type

Empty backbone

Tags

Cmyc and HA

Expression

Yeast

Available Since

Aug. 30, 2021

Availability

Academic Institutions and Nonprofits only

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