Skip to main content
Addgene
Showing: 7501 - 7520 of 21567 results

  9. pTJV1ScKm-rpoB

    Plasmid
    #166980
    Purpose
    Generates ssDNA in vivo targeting E. coli rpoB for recombineering w/ Beta recombinase; temp. sensitive pSC101 ori and sgRNA for Cas9 counterselection; npt2 for kanamycin resistance
    Depositor
    Christopher Reisch
    Article
    Ellington et al Synth Biol (Oxf). 2022 May 3;7(1):ysac00
    Insert
    NeoR/KanR
    Use
    CRISPR and Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    May 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    166980-plasmid-map-sequence-id-331333

    Enlarge

  14. pHEA

    Plasmid
    #168297
    Purpose
    Bacterial surface display of polypeptides (nanobodies) in E. coli
    Depositor
    Luis Ángel Fernández
    Article
    Marin et al J Bacteriol. 2010 Nov;192(21):5588-602.
    Insert
    Enterohemorraghic E. coli autotransporter EhaA C-terminal fragment (C-EhaA)
    Use
    Tags
    6xHis, E-tag, and PelB signal peptide
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 9, 2021
    Availability
    Academic Institutions and Nonprofits only
    168297-plasmid-map-sequence-id-334448

    Enlarge

  16. T444T-GFP(NCO)

    Plasmid
    #183972
    Purpose
    Enhanced RNAi clone for C. elegans knockdown of non-codon-optimized GFP
    Depositor
    David Matus
    Article
    Palmisano et al CourseSource, 2022
    Insert
    GFP (non-codon-optimized)
    Use
    RNAi
    Tags
    Expression
    Worm
    Mutation
    Promoter
    Available since
    July 31, 2023
    Availability
    Academic Institutions and Nonprofits only
    183972-plasmid-map-sequence-id-364073

    Enlarge

Showing: 7501 - 7520 of 21567 results