We narrowed to 14,584 results for: pET

1. pET24a His6 GST PYL2(14-188)

   Plasmid

   #177842

   Purpose

   Bacterial Expression of PYL2

   Depositor

   Karsten Melcher

   Article

   Melcher et al Nature. 2009 Dec 3;462(7273):602-8. doi:

   Insert

   PYR1-like 2 (PYL2 Mustard Weed)
   

   Use

   Tags

   6xHis-GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Dec. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET28a-Fgβ-anticalin-His-ybbr

   Plasmid

   #168040

   Purpose

   Expresses anticalin targeting CTLA-4 with Fgβ peptide at the N terminus and 6x-His tag and ybbr tag at the C terminus

   Depositor

   Michael Nash

   Article

   Liu et al Nano Lett. 2022 Jan 12;22(1):179-187. do

   Insert

   Anticalin
   

   Use

   Tags

   6x Histag, Fgβ peptide, and ybbr tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   July 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET15b p97-Strep-His E314Amb

   Plasmid

   #169021

   Purpose

   Expresses p97-Strep-His E314Amb (incorporates noncanonical amino acid at position 314 via amber suppression) in bacteria

   Depositor

   Hemmo Meyer

   Article

   Weith et al Mol Cell. 2018 Oct 11. pii: S1097-2765(1

   Insert

   p97
   

   Use

   Tags

   His and SBP

   Expression

   Bacterial

   Mutation

   E314TAG

   Promoter

   T7

   Available since

   July 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET51b-His-TEV-SNAP-tag_dead_mt

   Plasmid

   #167270

   Purpose

   Overexpression of the dead variant (C145A) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAP-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   C145A

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET51b-His-TEV-SNAP-tag_fast_dead_mt

   Plasmid

   #167272

   Purpose

   Overexpression of the fast (E30R) dead variant (C145A) of SNAP-tag in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   SNAPf-tag
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   E30R-C145A

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CPH3567 (pET151_Spt6 1247-1451 R1282H)

   Plasmid

   #91816

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with an R1282H mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed arginine 1282 to histidine; deleted resid…

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. CPH3495 (pET151_Spt6 1247-1451 K1435A)

   Plasmid

   #91817

   Purpose

   Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with a K1435A mutation

   Depositor

   Chris Hill

   Article

   Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

   Insert

   SPT6 (SPT6 Budding Yeast)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   changed lysine 1435 to alanine; deleted residues …

   Promoter

   T7

   Available since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-11a (GB1 WT - A34F/V39L)

   Plasmid

   #136610

   Purpose

   Expresses GB1 WT double mutant (A34F/V39L)

   Depositor

   Roberto Chica

   Article

   Damry et al Commun Biol. 2019 Nov 26;2:433. doi: 10.

   Insert

   GB1 WT double mutant (A34F/V39L)
   

   Use

   Tags

   6x-Histag

   Expression

   Bacterial

   Mutation

   Mutation from GB1 WT: A34F - V39L

   Promoter

   T7 promoter

   Available since

   Oct. 27, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-Synapsin-Ypet-3XNLS-WPRE

   Plasmid

   #159950

   Purpose

   nucleus marker

   Depositor

   Michael Courtney

   Article

   Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

   Insert

   Ypet-3XNLS
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   synthetic

   Promoter

   hSyn1

   Available since

   Oct. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. AAV-Synapsin-Ypet-optoJNKi-WPRE

    Plasmid

    #159967

    Purpose

    optogenetic JNK inhibitor with increased RET from LOV2 to reduce actuation sensitivity, detectable without activation

    Depositor

    Michael Courtney

    Article

    Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

    Insert

    Ypet-optoJNKi
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    synthetic

    Promoter

    hSyn1

    Available since

    Oct. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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