165,307 results

1. pcDNA3 HA E2F3A

   Plasmid

   #236437

   Purpose

   transient overexpression of E2F3A in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F3A (E2F3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB-CA

   Plasmid

   #20960

   Purpose

   piggyBac empty vector with Gatweway cassette and CAG constitutive promoter

   Depositor

   Andras Nagy

   Article

   Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

   Insert

   Gateway Destination Vector
   

   Use

   piggybac

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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3. LentiCRISPR-V2_hMSH2

   Plasmid

   #186155

   Purpose

   sgRNA targeting human MSH2 gene

   Depositor

   David McFadden

   Article

   Povedano et al Cell Chem Biol. 2019 Sep 19;26(9):1315-1

   Insert

   MSH2 (MSH2 Human)
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-hSyn-nucGFP

   Plasmid

   #140190

   Purpose

   Overexpression of GFP with a nuclear localization sequence, under the human synapsin promoter

   Depositor

   Lorenz Studer

   Article

   Miller et al Cell Stem Cell. 2013 Dec 5;13(6):691-705

   Insert

   GFP-NLS
   

   Use

   Lentiviral

   Tags

   GFP

   Expression

   Mammalian

   Available Since

   July 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. GAPDH

   Plasmid

   #155622

   Purpose

   For use in RBP tethering screen

   Depositor

   Eugene Yeo

   Article

   Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

   Insert

   GAPDH (GAPDH Human)
   

   Tags

   V5-MCP

   Expression

   Mammalian

   Mutation

   none

   Promoter

   EF-1a

   Available Since

   Nov. 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV.Syn.Flex.NES-jRGECO1a.WPRE.SV40

   Plasmid

   #100853

   Purpose

   AAV expression of Cre recombinase-activated jRGECO1a, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV Retrograde and AAV1

   Insert

   jRGECO1a
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   Nov. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMCSG53-DsbC

   Plasmid

   #186623

   Purpose

   E. coli expression vector containing N-terminal leaderless DsbC-6His-TEV fusion.

   Depositor

   Alexei Savchenko

   Article

   Venkatesan et al iScience. 2022 Sep 3;25(10):105054. doi:

   Type

   Empty backbone

   Tags

   DsbC-6His-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pmCherry Vinculin

   Plasmid

   #50527

   Depositor

   Kenneth Yamada

   Insert

   Vinculin (VCL Chicken)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCMV10-RBPJ

   Plasmid

   #84601

   Purpose

   Mammalian expression of FLAG tagged human RBPJ

   Depositor

   Yanan Yang

   Article

   Zhang et al Nat Commun. 2016 Jul 26;7:12231. doi: 10

   Insert

   RBPJ (RBPJ Human)
   

   Tags

   3xFlag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 4, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. SpCas9-CBE6c-V106W

    Plasmid

    #215826

    Purpose

    Express CBE6c V106W (with SpCas9) in mammalian cells

    Depositor

    David Liu

    Article

    Zhang et al Nat Commun. 2024 Feb 24;15(1):1697. doi:

    Insert

    CBE6c V106W-SpCas9-2xUGI (cas9 )
    

    Use

    In vitro transcription template

    Available Since

    March 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM60

    Plasmid

    #34610

    Depositor

    David Sabatini

    Insert

    deptor (DEPTOR Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 1, 2012

    Availability

    Academic Institutions and Nonprofits only

12. pLKO.1 3xHA-KillerRed-OMP25

    Plasmid

    #174544

    Purpose

    localizes KillerRed to mitochondria

    Depositor

    Minna Roh-Johnson

    Article

    Kidwell et al eLife. 2023 Mar 6;12:e85494. doi: 10.755

    Insert

    3xHA-KillerRed-OMP25
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. gRNA_AAVS1-T2

    Plasmid

    #41818

    Purpose

    Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

    Depositor

    George Church

    Article

    Mali et al Science. 2013 Feb 15;339(6121):823-6. do

    Insert

    gRNA_AAVS1-T2
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Jan. 11, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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14. pT7-CalfluxVTN

    Plasmid

    #83926

    Purpose

    Bioluminescent Reporter of Intracellular Calcium

    Depositor

    Carl Johnson

    Article

    Yang et al Nat Commun. 2016 Oct 27;7:13268. doi: 10

    Insert

    CalfluxVTN
    

    Expression

    Bacterial

    Mutation

    None

    Promoter

    T7

    Available Since

    Oct. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. dCas9-CBP core

    Plasmid

    #179543

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP core (aa 1084-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. Pyogenes dCas9 with c-terminal human CBP core (aa 1084-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pPtGE34

    Plasmid

    #107932

    Purpose

    Expresses Cas9 in Phaeodactylum tricornutum / Encodes elements required for conjugation

    Depositor

    David Edgell

    Article

    Slattery et al ACS Synth Biol. 2018 Jan 8. doi: 10.1021

    Inserts

    OriT
    40SRPS8 Promoter
    ShBle
    40SRPS8 Terminator
    Cen6-ArsH4-His3
    

    Use

    CRISPR and Synthetic Biology; Episomal vector for…

    Available Since

    Sept. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV-EF1a-BbChT

    Plasmid

    #45186

    Depositor

    Dawen Cai, Joshua Sanes

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-Ef1a-Brainbow/mCherry/mTFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Expression

    Mammalian

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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18. psPax2-D64V-NC-mNeon

    Plasmid

    #196509

    Purpose

    mNeon fluorescent protein fused to nucleocapsid protein in psPAX2

    Depositor

    Howard Chang

    Article

    Yu et al Cell. 2022 Dec 22;185(26):4904-4920.e22.

    Insert

    mNeon
    

    Tags

    mNeon

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRDB_053

    Plasmid

    #216083

    Purpose

    for transgenic flies; gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31, destination vector

    Depositor

    John Doench

    Article

    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

    Has Service

    Cloning Grade DNA

    Insert

    gypsy insulator sequences; white+ selectable marker; attB site for genome integration by phiC31
    

    Use

    Destination

    Expression

    Insect

    Available Since

    April 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HER2 CA (V659E)

    Plasmid

    #16259

    Depositor

    Mien-Chie Hung

    Article

    Li et al Cancer Cell. 2004 Nov . 6(5):459-69.

    Insert

    HER2 (ERBB2 Human)
    

    Expression

    Mammalian

    Mutation

    Constitutively active: V659E

    Available Since

    Nov. 30, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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