162,408 results

1. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pABE8e-protein

   Plasmid

   #161788

   Purpose

   Rhamnose-inducible expression of 8xHIS-ABE8e in E. coli

   Depositor

   David Liu

   Article

   Huang et al Nat Protoc. 2021 Feb;16(2):1089-1128. do

   Insert

   ABE8e base editor
   

   Use

   Tags

   8xHIS and BPNLS

   Expression

   Bacterial

   Mutation

   Promoter

   pRhaBAD

   Available since

   June 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pORANGE GFP-Camk2a KI

   Plasmid

   #131484

   Purpose

   Endogenous tagging of CaMKIIa: N-terminal (amino acid position: before startcodon)

   Depositor

   Harold MacGillavry

   Article

   Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

   Insert

   gRNA and GFP donor (Camk2a, Camk2a Mouse, Rat)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Cbh

   Available since

   Sept. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pAAV-hChATp-tdTomato

   Plasmid

   #197564

   Purpose

   Expresses tdTomato under a human choline acetyltransferase (hChAT) promoter fragment

   Depositor

   Casey Maguire

   Article

   Santoscoy et al Mol Ther Methods Clin Dev. 2023 May 9;29

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   human ChAT promoter

   Available since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCbs FlagOmomyc

   Plasmid

   #113168

   Purpose

   Expression of FLAG tagged Omomyc in mammalian cells

   Depositor

   Sergio Nasi

   Article

   Savino et al PLoS One. 2011;6(7):e22284. doi: 10.1371

   Insert

   Omomyc (MYC Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   E57T, E64I, R70Q, R71N

   Promoter

   Available since

   Aug. 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. INCTbiosyn-pUB-HspINTphiC31

   Plasmid

   #127518

   Purpose

   Plasmid encodes H. sapiens codon optimized Integrase phiC31.

   Depositor

   Martin Bonamino

   Article

   Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

   Insert

   Integrase phiC31 coding sequence codon optimized for H. sapiens expression.
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-rtTA

   Plasmid

   #175274

   Purpose

   AAV vector mediating neuron-specific expression of rtTA

   Depositor

   Wei Xu

   Article

   Li et al Nat Methods. 2021 Nov 25. pii: 10.1038/s

   Insert

   Reverse tetracycline-controlled transactivator (rtTA)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   Synapsin

   Available since

   Nov. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. #2.4 pre-G-SynapShot-TagBFP

   Plasmid

   #214721

   Purpose

   Expresses green #2.4 pre-SynapShot and TagBFP

   Depositor

   Won Do Heo

   Article

   Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

   Insert

   #2.4 pre-G-SynapShot
   

   Use

   AAV

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. Cilantro 2

   Plasmid

   #74450

   Purpose

   Can clone in a C2H2 zinc finger via BsmBI restriction sites and monitor post-translational degradation using EGFP:mCherry ratio (Lentiviral, PGK.BsmBICloneSite-EGFP.IRES.mCherry.cppt.EF1a.PuroR)

   Depositor

   Benjamin Ebert

   Article

   Slabicki et al Nature. 2020 Sep;585(7824):293-297. doi:

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mutation

   Promoter

   Available since

   July 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-FKBP-MTM1

    Plasmid

    #51614

    Purpose

    Recruitable PtdIns3P/PtdIns(3,5)P2 phosphatase

    Depositor

    Tamas Balla

    Article

    Hammond et al J Cell Biol. 2014 Apr 7.

    Inserts

    FKBP
    MTM1 (MTM1 1812, Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 26, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. pECE

    Plasmid

    #26453

    Depositor

    William Rutter

    Article

    Ellis et al Cell. 1986 Jun 6;45(5):721-32.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3 Connexin43-GFP-APEX2

    Plasmid

    #49385

    Purpose

    Expresses a tandem GFP-APEX2 fusion to the C-terminus of connexin43 in mammalian cells

    Depositor

    Alice Ting

    Article

    Lam et al Nat Methods. 2014 Nov 24. doi: 10.1038/n

    Insert

    Connexin43 - EGFP - APEX2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K14D, W41F, E112K, A134P on soybean APX; improved…

    Promoter

    CMV

    Available since

    Nov. 27, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

    Plasmid

    #198240

    Purpose

    For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

    Depositor

    Didier Stainier

    Article

    Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

    Inserts

    Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)
    

    Use

    Zebrafish transgenesis

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. EasyFusion T2A-H2B-mCherry

    Plasmid

    #113089

    Purpose

    General donor vector with T2A-H2B-mCherry coding sequence flanking by restriction sites for generating knock-in donor

    Depositor

    Janet Rossant

    Article

    Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

    Insert

    T2A-H2B-mCherry
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCI152-pLenti-SiT-Cas12a

    Plasmid

    #128405

    Purpose

    Express both AsCas12a and CRISPR arrays on single transcripts

    Depositor

    Randall Platt

    Article

    Campa et al Nat Methods. 2019 Aug 12. pii: 10.1038/s

    Insert

    AsCas12a-Triplex
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF1116

    Plasmid

    #143255

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RCOR1 (RCOR1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. ICMAB - pET22b T7 EncFtn Mms6

    Plasmid

    #225538

    Purpose

    Construction of intracellular magnetite accumulating bacterial systems.

    Depositor

    Urartu Seker

    Article

    Yavuz et al Small. 2022 Jul;18(26):e2200537. doi: 10

    Inserts

    Iron binding protein
    Ferroxidase
    Material binding peptite
    Iron transporter protein
    

    Use

    Tags

    no

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Feb. 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pMOS015E: Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Plasmid

    #163076

    Purpose

    Entry vector for: mito-roGFP2-Orp1 H2O2 oxidation sensor (mitochondrial)

    Depositor

    Adam Cohen

    Article

    Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

    Insert

    mito-roGFP2-Orp1
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP (LLH528)

    Plasmid

    #208289

    Purpose

    CMV and T7 promoter expression plasmid for human codon optimized ABE8e A-to-G base editor with iSpyMac(D10A) and P2A-EGFP

    Depositor

    Benjamin Kleinstiver

    Article

    Alves et al Nat Biomed Eng. 2023 Dec 6. doi: 10.1038

    Insert

    pCMV-T7-BPNLS-ABE8e-iSpyMac-BPNLS-P2A-EGFP
    

    Use

    CRISPR; In vitro transcription; t7 promoter

    Tags

    BPNLS and BPNLS-P2A-EGFP

    Expression

    Mammalian

    Mutation

    ABE8e mutations in TadA; mutations in iSpyMac

    Promoter

    CMV and T7

    Available since

    Dec. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pINDUCER21-PGBD5

    Plasmid

    #78121

    Purpose

    Lentiviral gene expression vector for doxycycline inducible PGBD5 expression

    Depositor

    Alex Kentsis

    Article

    Henssen et al Nat Genet. 2017 May 15. doi: 10.1038/ng.

    Insert

    PGBD5 (PGBD5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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