167,150 results
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Plasmid#205411PurposeVector encoding human codon-optimized enhanced-activity OgeuIscB fused with T5E at C-terminal driven by CAG promoter, optimized _RNA (_RNA*) driven by hU6, and mCherry driven by CMV promoterDepositorInsertpCAG_Flag_SV40NLS_OgeuIscB*_XTEN_T5E_npNLS_pU6__RNA*_pCMV_mCherry
ExpressionMammalianMutationE85R+H369R+S387R+S457RPromoterCAG, hU6, CMVAvailable SinceDec. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG.CASP3-Myc
Plasmid#200119PurposeExpresses C-terminal Myc-tagged caspase 3 in mammalian cellsDepositorAvailable SinceMay 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-N1-FUS/TLS-FLAGC
Plasmid#60362PurposePlasmid for expression of FLAG-GFP tagged human FUS/TLS (C-terminal tag). Confers resistance to G418.DepositorAvailable SinceJan. 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
cfSGFP2-anti-AlfaTag nanobody
Plasmid#171818PurposeBacterial expression vector for production of fluorescent anti-AlfaTag nanobodyDepositorInsertcfSGFP2-anti-AlfaTag nanobody
Tags6xHis and SEP tag (6xD)ExpressionBacterialAvailable SinceJune 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
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pRDA_867
Plasmid#231109PurposeA>G base editorDepositorInsertABE8e-nCas9-SpG-HA
UseCRISPR and LentiviralMutationD10AAvailable SinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpyTag003-Talin rod-mCherry
Plasmid#133567PurposeExpresses SpyTag003-Talin rod-mCherry in mammalian cellsDepositorInsertSpyTag003-Talin rod-mCherry
ExpressionMammalianPromoterCMVAvailable SinceDec. 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET21a(+)-Bst LF-6xHis
Plasmid#159148Purposeexpresses His-tagged large fragment of Bst polymeraseDepositorInsertlarge fragment of Geobacillus stearothermophilus DNA polymerase I
TagsHisExpressionBacterialAvailable SinceSept. 1, 2020AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMU-ACTFr
Plasmid#61191PurposeFluorescent reporter for F-actin AtFim-ABD2-mCherry expressed under AtUBQ10 promoterDepositorInsertAtFimbrin1-ABD
TagsmCherryExpressionPlantPromoterAtUBQ10Available SinceFeb. 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
FUGW-eGFP-ZEB1 3'UTR
Plasmid#81018PurposeFUGW lentiviral vector with constitutively expressed eGFP regulated by the human Zeb1 3'UTR. Brighter than destabilized d2GFP sensor reported in manuscriptDepositorInsertZeb1 3'UTR (UTS2R Human)
UseLentiviralAvailable SinceAug. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
DOX_SAM_CRISPR-activation-2A-GFP-AAVS1-targeting-construct
Plasmid#176836PurposeTargeting construct for intergrating a doxycycline (DOX) inducible SAM CRISPR activation complex -2A-GFP to the AAVS1 locus.DepositorInsertdCas9-VP64-2A-MS2-p65-HSF1-2A-GFP
UseUnspecifiedMutationSee Depositor Comments BelowPromoterTet operator (tetracycline inducible expression)Available SinceNov. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
Membrane mCherry
Plasmid#210470PurposePlasma membrane localizing mCherry using Human LCK proto-oncogene sequenceDepositorInsertLCK membrane mCherry (LCK Human, Synthetic, A.victoria)
UseSynthetic Biology; Sea urchin, mammal, zebrafishTagsmCherryAvailable SinceDec. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
nls-EGFP
Plasmid#67652PurposeMammalian expression vector driving nls-EGFP.DepositorInsertnls-EGFP
ExpressionMammalianPromoterCMVAvailable SinceNov. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
dCas12j3 VPR
Plasmid#188517PurposeExpresses FLAG tagged dCas12j3 VPRDepositorInsertdCas12j3
UseCRISPR and LentiviralExpressionMammalianMutationD413APromoterEF1aAvailable SinceNov. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPB-cT3G-cERP2-YTHDC2
Plasmid#222558PurposePiggyBac transposon plasmid for doxycycline inducible expression of YTHDC2DepositorInsertYTHDC2 (YTHDC2 Human)
ExpressionMammalianAvailable SinceAug. 13, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCE35
Plasmid#174409PurposeC. auris LEUpOUT NAT marker CAS9 expression construct. Use with pCE27 gRNA expression construct.DepositorInsertC. auris LEU2 1 of 2, pENO1, Cas9, NAT 1 of 2
UseCRISPRExpressionBacterialAvailable SinceDec. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMEH371 CD63 HA
Plasmid#168220PurposeMammalian expression of tetraspanin CD63 fused to a C-terminal HA tagDepositorAvailable SinceDec. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
HA-TRIM28deltaLinker
Plasmid#124958PurposeMammalian expression of Human TRIM28 with deleted Linker region between CC and PHD domainsDepositorInserthTRIM28 (TRIM28 Human)
TagsHAExpressionMammalianMutationDeletion of linker region between CC domain and P…Available SinceMay 3, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-PE2-P2A-hMLH1dn
Plasmid#174827PurposeMammalian expression of SpCas9 prime editor 2 with P2A-human MLH1dn (codon optimized)DepositorInsertPE2-P2A-hMLH1dn
TagsSV40 bpNLSExpressionMammalianMutationDetailed in manuscript; deletion of residues 754-…PromoterCMVAvailable SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pLVET-IRES-GFP
Plasmid#107139PurposeA bicistronic lentiviral mammalian expression vector with a multiple cloning site and GFPDepositorTypeEmpty backboneUseLentiviralExpressionMammalianAvailable SinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits only