173,520 results
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Plasmid#103865PurposeCatalytically inactive PSPCas13b. RNase activity is inactivated by mutation of catalytic histidine residues in the HEPN domains (H133A/H1058A).DepositorInsertPspCas13b
UseCRISPR and LentiviralTags3xHA and HIV NESExpressionMammalianMutationContains the H133A and H1058A mutations which ina…PromoterEF1aAvailable SinceJan. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
MGAT-SEP-mRuby3
Plasmid#200937Purposecis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3DepositorInsertsUseLentiviralAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHCMV_DENV4_PrME
Plasmid#207266PurposeMammalian expression of Dengue virus 4 PrME proteinDepositorInsertDENV4_PrME (POLY Dengue virus 4)
ExpressionMammalianMutationmammalian codon optimizationPromoterCMV promoterAvailable SinceNov. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pATT-Dest
Plasmid#79770PurposeStaging plasmid for use in DIP transpositionDepositorTypeEmpty backboneUseSynthetic Biology; Staging plasmid for transposit…Available SinceJuly 13, 2016AvailabilityAcademic Institutions and Nonprofits only -
SpRY-ABE8e(V106W)
Plasmid#198553PurposeExpresses human codon-optimized SpRY-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpRY-ABE8e(V106W)-NLS-FLAG-P2A-BSDDepositorInsertSpRY-ABE8e(V106W)
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable SinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CaMKIIa-hM4D(Gi)-mCherry
Plasmid#50477PurposeGi-coupled hM4D DREADD fused with mCherry under the control of CaMKIIa promoterDepositorHas ServiceAAV1, AAV2, AAV5, AAV8, and AAV9InserthM4D(Gi)-mCherry (CHRM4 Human)
UseAAVTagsmCherryMutationSee supplemental documents for DREADD mutationsPromoterCaMKIIaAvailable SinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMK1334
Plasmid#127965PurposesgRNA expression vector compatible with CROP-Seq and imaging screensDepositorInsertEF1a-Puro-T2A-2xmycNLS-WPRE-mU6-sgRNA
UseCRISPR and LentiviralExpressionMammalianPromotermU6Available SinceAug. 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
EZH2 E10.3 gRNA
Plasmid#90685Purpose3rd generation lentiviral gRNA plasmid targeting human EZH2DepositorInsertEZH2 (Guide Designation E10.3)
UseCRISPR and LentiviralPromoterU6Available SinceJuly 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
EGFP-p300
Plasmid#191764PurposeExpression of EGFP fused to core p300 histone acetyltransferaseDepositorInsertFRB-EGFP-p300 core (EP300 Human)
TagsEGFP (N-terminal of p300 core) and FRB (N-termina…ExpressionBacterial and MammalianPromoterEF1alphaAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGWB501
Plasmid#74843PurposeGateway cloning compatible binary vector for simple cloning of gene.DepositorTypeEmpty backboneExpressionPlantPromoterNo PromoterAvailable SinceAug. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
TFORF2340
Plasmid#142039PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
TFORF2442
Plasmid#142080PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorAvailable SinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDe-CAS9
Plasmid#61433PurposeBinary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequenceDepositorInsertCas9
UseCRISPRExpressionPlantPromoterPcUBI4-2Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pLVTHM
Plasmid#12247Purpose2nd gen lentivector expressing shRNA from H1 promoter and GFP. For direct cloning of shRNA or sub-cloning of an H1-shRNA cassette from pSUPER. Dox-controllable if used with tTRKRAB expressing plasmidDepositorHas ServiceCloning Grade DNATypeEmpty backboneUseCre/Lox, Lentiviral, and RNAiExpressionMammalianAvailable SinceAug. 4, 2006AvailabilityAcademic Institutions and Nonprofits only -
pSEVA63-GG
Plasmid#208015Purposefor cloning homology arms, with GFP as counter selection markerDepositorInsertmsfGFP
Promoter14GAvailable SinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRSET-iGluSnFR4f
Plasmid#234444PurposeBacterial expression of glutamate sensor with with improved sensitivity and fast deactivation kinetics (4f = fast)DepositorInsertiGluSnFR4f
Tags6xHis-Xpress epiExpressionBacterialPromoterT7Available SinceMarch 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-iLACCO1.1
Plasmid#218822PurposeMammalian expression of genetically encoded green fluorescent L-lactate biosensor with low affinityDepositorInsertiLACCO1.1
ExpressionMammalianAvailable SinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pegRNA-GFP>BFP_Y66H-NGG
Plasmid#185480PurposepegRNA plasmid in order to make a Y66H (TAC>CAT) conversion in the GFP gene, creating a BFP gene.DepositorInsertGFP>BFP_Y66H-pegRNA
ExpressionMammalianPromoterU6Available SinceJuly 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Empty)-PGKBFP2AGFP-W
Plasmid#67979PurposeCas9 activity reporter (control) with BFP and GFP.DepositorInsertU6gRNA cassette, PGKBFP2AGFP cassette, WPRE
UseLentiviralMutationDeleted BbsI site within WPREPromoterhuman U6 promoter, mouse PGK promoterAvailable SinceSept. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
Nluc CD63
Plasmid#242528PurposeThis plasmid can be used to quantify CD63 by luminescence signal upon providing substrate, in particular in the secretome, especially in the extracellular vesicles of cells expressing this construct.DepositorAvailable SinceFeb. 23, 2026AvailabilityAcademic Institutions and Nonprofits only