Addgene

We narrowed to 6,032 results for: WUS;

Showing: 1111 - 1140 of 6032 results

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pCas4_1_Δ2_VA

Plasmid

#186439

Purpose

Encodes Cas4, Cas1, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4_1_2_VA

Plasmid

#186441

Purpose

Encodes Cas4 (K70A), Cas1, Cas2, leader, repeat and one spacer from F. Novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4, Cas1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1_2_VB

Plasmid

#186442

Purpose

Encodes Cas4/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-4/1Δ2_VB

Plasmid

#186443

Purpose

Encodes Cas4/1, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1

Use

CRISPR

Tags

Expression

Mutation

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas-mut4/1_2_VB

Plasmid

#186444

Purpose

Encodes Cas4(K81A)/1, Cas2, leader, repeat and one spacer from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas4/1 and Cas2

Use

CRISPR

Tags

Expression

Mutation

Cas4 domain (K81A)

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12(PI)

Plasmid

#186448

Purpose

Encodes inactive Cas12a containing mutation in the PI domain (K613A, K617A) from F. novicida (type V-A)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12a (PI)

Use

CRISPR

Tags

Expression

Mutation

K613A, K617A

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCas12b(PI)

Plasmid

#186451

Purpose

Encodes inactive Cas12b containing mutation in the PI domain (R122A, G143P) from A. acidoterrestris (type V-B)

Depositor

John van der Oost

Article

Wu et al CRISPR J. 2022 Aug;5(4):536-547. doi: 10

Insert

Cas12b (PI)

Use

CRISPR

Tags

Expression

Mutation

R122A, G143P

Promoter

T7

Available since

March 20, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FRISZ-GPI

Plasmid

#177264

Purpose

AAV transfer plasmid for cell-surface-localized FRISZ (via CD59 GPI) under hSyn promoter

Depositor

Huiwang Ai

Article

Wu et al Sci Adv. 2023 Mar;9(9):eadd2058. doi: 10

Insert

CD59 leader-FRISZ-GPI

Use

AAV

Tags

CD59 GPI and CD59 leader sequence

Expression

Mutation

Promoter

hSyn

Available since

Feb. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-HTR2A-NTEV-TCS-GV-2xHA

Plasmid

#194367

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194368

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-HTR2A-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194369

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-HTR2A-V2R-NTEV-TCS-GV-2xHA (HTR2A Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-AVPR2-NTEV-TCS-GV-2xHA

Plasmid

#194371

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-AVPR2-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194372

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-AVPR2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194373

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-AVPR2-V2R-NTEV-TCS-GV-2xHA (AVPR2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3_SP-GCGR-NTEV-TCS-GV-2xHA

Plasmid

#194375

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GCGR-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_GCGR-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194376

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

GCGR-V2R-NTEV-TCS-GV-2xHA (GCGR Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-GLP1R-NTEV-TCS-GV-2xHA

Plasmid

#194379

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-GLP1R-NTEV-TCS-GV-2xHA (GLP1R Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-DRD1-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194361

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD1-V2R-NTEV-TCS-GV-2xHA (DRD1 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-DRD2-NTEV-TCS-GV-2xHA

Plasmid

#194363

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD2-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_DRD2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194364

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA3_SP-DRD2-V2R-NTEV-TCS-GV-2xHA

Plasmid

#194365

Purpose

Split TEV assays

Depositor

Michael Wehr

Article

Wu et al Biosensors (Basel). 2022 Dec 29;13(1):48

Insert

SP-DRD2-V2R-NTEV-TCS-GV-2xHA (DRD2 Synthetic, Human)

Use

Tags

2xHA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
mKate2-DrCavin1b in pT3TS-Dest

Plasmid

#194295

Purpose

In vitro transcription of mKate2 tagged zebrafish cavin1b from the T3 promoter. The red fluorescent tag is at the N-terminus. Parton lab clone LAC

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

In vitro transcription of mrna

Tags

Expression

Mutation

Promoter

Available since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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p3E-DrCavin1b

Plasmid

#194292

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish cavin1b with fusion tag at the N-terminus. Parton lab clone KXJ

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

cavin1b (cavin1b Zebrafish)

Use

Multisite gateway entry vector

Tags

Expression

Mutation

Promoter

Available since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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tetO-Fzd1-IRES-tdTomato (TET006)

Plasmid

#160510

Purpose

This plasmid expresses Fzd1 under the activation of tTA trans-activator along with fluorescent marker tdTomato.

Depositor

C. Ron Yu

Article

Wu et al bioRxiv 2022

Insert

Fzd1 (Fzd1 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-CD45

Plasmid

#175943

Purpose

knockout mouse CD45

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

CD45 gRNA (Ptprc Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-Tpi1

Plasmid

#175940

Purpose

knockout mouse Tpi1

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Tpi1 gRNA (Tpi1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pSIN-Olfr2

Plasmid

#175935

Purpose

knockout mouse Olfr2

Depositor

Dan Littman

Article

Wu et al Cell. 2020 Aug 6;182(3):641-654.e20. doi

Insert

Olfr2 gRNA (Or6a2 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
human ETV6 gRNA-3

Plasmid

#133403

Purpose

human ETV6 gRNA-3 is a 20-nt gRNA expression plasmid targeting the second ETV6 exon.

Depositor

Linzhao Cheng

Article

Wu et al Stem Cell Res. 2018 May;29:6-14. doi: 10

Insert

ETV6 (ETV6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3-SPA mimic WT

Plasmid

#92350

Purpose

To mimic SPA RNA processing and expression

Depositor

Ling-Ling Chen

Article

Wu et al Mol Cell. 2016 Nov 3;64(3):534-548. doi:

Insert

SNRPN exon 9, intron9, exon10, intron10 partial partial of SPA1 (SNRPN Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pDZ306 (GLT1 POL1 24xPP7SL)

Plasmid

#35196

Depositor

Robert Singer

Article

Larson et al Science. 2011 Apr 22;332(6028):475-8.

Insert

GLT1 POL1 24xPP7SL

Use

Tags

Expression

Mutation

Promoter

Available since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge