We narrowed to 2,810 results for: ALC

1. mCherry-NES-SspB-MCS-P2A-iLIDcaax

   Plasmid

   #173869

   Purpose

   Mammalian expression of mCherry-NES-SspB-P2A-iLIDcaax (opto-control) containing multiple cloning sites (MCS)

   Depositor

   Takao Nakata

   Article

   Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

   Insert

   mCherry-NES-SspB-MCS-P2A-iLIDcaax
   

   Use

   Tags

   C-terminal CAAX-box and mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pPBC-G6f

   Plasmid

   #62811

   Purpose

   pPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-GCaMP6f-ITR
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDuex2-iBB-GECO1

   Plasmid

   #194216

   Purpose

   Far-red fluorescent Ca2+ ion indicator for mammalian cell and bacterial expression.

   Depositor

   Robert Campbell

   Article

   Hashizume et al Protein Sci. 2022 Oct;31(10):e4440. doi:

   Insert

   iBB-GECO1
   

   Use

   Tags

   6xHis

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Feb. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pPBC-LG6s

   Plasmid

   #62809

   Purpose

   pPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.

   Depositor

   Karen Wilcox

   Article

   Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

   Insert

   ITR-CAG-Lck-GCaMP6s-ITR
   

   Use

   Tags

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. CathepsinG-CD4d3+4-bio

   Plasmid

   #73125

   Purpose

   EXPRESs plasmid for human erythrocyte surface proteins encoding CathepsinG with rat CD4d3+4-bio

   Depositor

   Gavin Wright

   Article

   Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

   Insert

   Cathepsin G (CTSG Human)
   

   Use

   Tags

   biotinylation peptide and ratCD4d3+4

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJRoC30_Th3

   Plasmid

   #188064

   Purpose

   PROSS-designed high-redox potential laccase from Trametes hirsuta

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes hirsuta
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   20 mutations, described in publication

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJRoC30_Tv9nL

   Plasmid

   #188069

   Purpose

   PROSS-designed high-redox potential laccase from Trametes versicolor

   Depositor

   Sarel Fleishman

   Article

   Barber-Zucker et al ACS Catal. 2022 Nov 4;12(21):13164-13173

   Insert

   PROSS-designed high-redox potential laccase from Trametes versicolor
   

   Use

   Tags

   S. cerevisiae evolved α-factor prepro-leader sign…

   Expression

   Yeast

   Mutation

   79 PROSS mutations, described in publication

   Promoter

   Available since

   Oct. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28_6xHis-PARP1delCAT

   Plasmid

   #173942

   Purpose

   Bacterial expression of PARP1 lacking CAT domain

   Depositor

   Joan Conaway

   Article

   Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

   Insert

   PARP1delCAT (PARP1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   Deletion of residues 661-1014

   Promoter

   Available since

   Sept. 30, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pTol2 - myo6b:CaSR-EGFP, cryaa:mCherry

    Plasmid

    #191500

    Purpose

    GROW AT 30 DEGREES. A construct used to create transgenic fish that express CaSR-EGFP in hair cells and an mCherry marker in the lens of the eye

    Depositor

    Michael Granato

    Article

    Shoenhard et al Cell Rep. 2022 Dec 6;41(10):111790. doi:

    Insert

    CaSR-EGFP-SV40
    

    Use

    Creation of transgenic fish using tol2 transgenes…

    Tags

    Expression

    Mutation

    Promoter

    myo6b

    Available since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28_6xHis-PARP1delWGR

    Plasmid

    #173940

    Purpose

    Bacterial expression of PARP1 lacking WGR domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delWGR (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 521-660; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCSF107mT-GATEWAY-3'-LAP tag

    Plasmid

    #67618

    Purpose

    A pCS2 based vector to generate a 3'-LAP (eGFP-TEV-S) tag using GATEWAY cloning technology

    Depositor

    Todd Stukenberg

    Article

    Grant et al Dev Biol. 2015 Dec 15;408(2):345-57. doi

    Type

    Empty backbone

    Use

    In vitro transcription or translation off of an s…

    Tags

    C-termnal LAP (eGFP-TEV-S)

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and SP6

    Available since

    Aug. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pPBC-LG6f

    Plasmid

    #62808

    Purpose

    pPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-Lck-GCaMP6f-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pPBC-G3-tdT

    Plasmid

    #62814

    Purpose

    pPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.

    Depositor

    Karen Wilcox

    Article

    Gee et al Front Mol Neurosci. 2015 Apr 15;8:10. do

    Insert

    ITR-CAG-GCaMP3-IRES-tdTomato-ITR
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pBMN HA-NDP52

    Plasmid

    #199307

    Purpose

    For expression of the mammalian NDP52 tagged with HA

    Depositor

    Michael Lazarou

    Article

    Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Carl-CeNL(Ca2+)_110μ-KDEL-pcDNA3

    Plasmid

    #111925

    Purpose

    Cyan color luminescent Ca2+ indicator targeted to endoplasmic reticulum. Calreticulin and a KDEL signal for ER retention located at the N-terminus and C-terminus of CeNL(Ca2+)_110μ

    Depositor

    Takeharu Nagai

    Article

    Hossain et al ACS Chem Biol. 2018 Mar 9. doi: 10.1021/

    Insert

    CeNL(Ca2+)_110μ
    

    Use

    Tags

    Calreticulin and KDEL

    Expression

    Mammalian

    Mutation

    E31D, F92W, E104D. D133E at CaM

    Promoter

    CMV

    Available since

    June 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pBMN mCherry-NDP52(C443K)

    Plasmid

    #119685

    Purpose

    For mammalian expression of UBD mutant NDP52

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    C443K

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pBMN mCherry-NDP52(V136S/C443K)

    Plasmid

    #119686

    Purpose

    For mammalian expression of LIR/UBD double mutant NDP52

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V136S/C443K

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pBMN mEGFP-NDP52(V136S)

    Plasmid

    #119682

    Purpose

    For mammalian expression of LIR mutant NDP52

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    NDP52 (CALCOCO2 Human)
    

    Use

    Retroviral

    Tags

    mEGFP

    Expression

    Mammalian

    Mutation

    V136S

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax

    Plasmid

    #173874

    Purpose

    Mammalian expression of mVenus-NES-SspB-C3Gcat-P2A-iLIDcaax (opto-Rap)

    Depositor

    Takao Nakata

    Article

    Inaba et al J Biol Chem. 2021 Jan-Jun;296:100290. do

    Insert

    mCherry-NES-SspB-C3Gcat-P2A-iLIDcaax
    

    Use

    Tags

    C-terminal CAAX-box and mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pEGFPC1-EPSIN 2

    Plasmid

    #22242

    Depositor

    Pietro De Camilli

    Article

    Rosenthal et al J Biol Chem. 1999 Nov 26. 274(48):33959-

    Insert

    Epsin 2 (EPN2 Human)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 3, 2009

    Availability

    Academic Institutions and Nonprofits only

22. pFlox_N-Mau2_Neo_FKBPF36V_mScarlet

    Plasmid

    #163881

    Purpose

    Acute Depletion of Mau2-mScarlet

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    Mau2 (Mau2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 19, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pET28_6xHis-PARP1delZn3

    Plasmid

    #173936

    Purpose

    Bacterial expression of PARP1 lacking Zn3 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn3 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 214-373; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCR4-huNFATc1nuc

    Plasmid

    #23988

    Depositor

    Jerry Crabtree

    Article

    Winslow et al Dev Cell. 2006 Jun . 10(6):771-82.

    Insert

    NFATc1 (NFATC1 Human)
    

    Use

    Cloning vector

    Tags

    EE tag

    Expression

    Mutation

    S172A S175A S176A S178A S179A S181A S184A S187A S…

    Promoter

    Available since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pAAV-Htr5b(3.7)-EGFP-sv40

    Plasmid

    #184411

    Purpose

    AAV expression of EGFP from mouse 5-hydroxytryptamine (serotonin) receptor 5B (Htr5b) gene promoter.

    Depositor

    Marylka Yoe Uusisaari

    Article

    Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

    Insert

    Mouse Htr5b gene promoter-EGFP (Htr5b Mouse)
    

    Use

    AAV

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Mouse Htr5b gene

    Available since

    Aug. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pBMN mCherry-TAX1BP1(V142S/ΔUBD)

    Plasmid

    #119690

    Purpose

    For mammalian expression of LIR mutant TAX1BP1 without its UBD

    Depositor

    Michael Lazarou

    Article

    Padman et al Nat Commun. 2019 Jan 24;10(1):408. doi:

    Insert

    TAX1BP1 (TAX1BP1 Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    V142S & UBD deletion (1-755), See depositor c…

    Promoter

    Available since

    Jan. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. Krox20-MSE8 pGL3-TATA

    Plasmid

    #21261

    Depositor

    Jerry Crabtree

    Article

    Kao et al Science. 2009 Jan 30. 323(5914):651-4.

    Insert

    Krox20-Myelin Specific Enhancer (Egr2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 28, 2009

    Availability

    Academic Institutions and Nonprofits only

28. GlycophorinB-CD4d3+4-bio

    Plasmid

    #73094

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding GlycophorinB with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Glycophorin B (GYPB Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 25, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR-D-TOPO-kcnn3

    Plasmid

    #164959

    Purpose

    Zebrafish kcnn3 gene CDS

    Depositor

    GuangJun Zhang

    Article

    Silic et al Dev Dyn. 2021 Mar 16. doi: 10.1002/dvdy.

    Insert

    kcnn3 (kcnn3 Zebrafish)
    

    Use

    Pcr cloning vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcdna DSG-2 Tailless

    Plasmid

    #89466

    Purpose

    DSG-2 tailless control

    Depositor

    Daniel Conway

    Article

    Baddam et al Cells. 2018 Jun 26;7(7). pii: cells70700

    Insert

    desmoglein-2 (DSG2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. px330 NIPBL gRNA

    Plasmid

    #165590

    Purpose

    Insertion of NIPBL degron

    Depositor

    Cornelis Murre

    Article

    Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

    Insert

    NIPBL (Nipbl Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    NA

    Promoter

    Available since

    March 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. sfGFP-SasG(8G)-mCherry

    Plasmid

    #206950

    Purpose

    Mammalian expression of heterobifunctional SasG linkers (8G)

    Depositor

    Gil Westmeyer

    Article

    Sigmund et al Nat Biotechnol. 2023 Apr 17. doi: 10.103

    Insert

    sfGFP-SaSasG (8G)-mCherry
    

    Use

    Tags

    mCherry and sfGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. Semaphorin7A-CD4d3+4-bio

    Plasmid

    #73115

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Semaphorin7A with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Semaphorin 7A (SEMA7A Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. OA-1067A1

    Plasmid

    #164847

    Purpose

    Expresses four gRNA targeting BTub gene with 3xp3-eGFP marker

    Depositor

    Omar Akbari

    Article

    Li et al Nat Commun. 2021 Sep 10;12(1):5374. doi:

    Insert

    4 gRNAs and 3xP3-eGFP
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCAG-hPPP3R1

    Plasmid

    #179136

    Purpose

    Expression vector of human protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1), CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, regulatory subunit B, alpha isoform (PPP3R1 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pET28_6xHis-PARP1delZn1

    Plasmid

    #173933

    Purpose

    Bacterial expression of PARP1 lacking Zn1 domain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1delZn1 (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Deletion of residues 1-96; V762A

    Promoter

    Available since

    Sept. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pET28_6xHis-PARP1-HD

    Plasmid

    #174809

    Purpose

    Bacterial expression of isolated PARP1 HD subdomain

    Depositor

    Joan Conaway

    Article

    Ooi et al Proc Natl Acad Sci U S A. 2021 Sep 7;118

    Insert

    PARP1-HD (PARP1 Human)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    V762A

    Promoter

    Available since

    Sept. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. Neuroplastin-CD4d3+4-bio

    Plasmid

    #73111

    Purpose

    EXPRESs plasmid for human erythrocyte surface proteins encoding Neuroplastin with rat CD4d3+4-bio

    Depositor

    Gavin Wright

    Article

    Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

    Insert

    Neuroplastin (NPTN Human)
    

    Use

    Tags

    biotinylation peptide and ratCD4d3+4

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pGEX-6P-1-hs-ALPS 1-ALPS2-mCherry

    Plasmid

    #187114

    Purpose

    Protein Expression

    Depositor

    Philipp Niethammer

    Article

    Shen et al Proc Natl Acad Sci U S A. 2022 Jan 4;119

    Insert

    ENSG00000101199 (ARFGAP1 Human)
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Aug. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pTol2-elavl3-WHaloCaMP1a

    Plasmid

    #205312

    Purpose

    Expression of WHaloCaMP1a in zebrafish from the elavl3 promoter (pan-neuronal expression)

    Depositor

    Eric Schreiter

    Article

    Farrants et al bioRxiv. 2023 Jul 19:2023.07.18.549527.

    Insert

    WHaloCaMP1a
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge