We narrowed to 7,580 results for: Ski;

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pAAV-EF1a-hfCas13d-pa

Plasmid

#233037

Purpose

To express hfCas13d from a EF1a promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d

Use

AAV

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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pPB9

Plasmid

#210036

Purpose

Contains Cas9 gRNA cloning site, inducible Cas9, UCOE, puro-T2A-eGFP

Depositor

Michael Talkowski

Article

Nuttle et al Cell Rep Methods. 2023 Dec 5:100672. doi

Inserts

Cas9
puro-T2A-eGFP

Use

CRISPR

Tags

SV40 NLS and nucleoplasmin NLS

Expression

Mammalian

Promoter

EF-1α and TRE3G

Available Since

Feb. 6, 2025

Availability

Academic Institutions and Nonprofits only

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p204-U6-Switch-ON

Plasmid

#217883

Purpose

Retroviral Switch-ON vector for sgRNA expression; U6-BbsIx2-SWITCH-ON-scaffold; neoR

Depositor

Ulrich Elling

Article

Chylinski et al Nat Commun. 2019 Nov 29;10(1):5454. doi:

Type

Empty backbone

Use

CRISPR, Cre/Lox, and Retroviral

Expression

Mammalian

Promoter

hU6

Available Since

May 17, 2024

Availability

Academic Institutions and Nonprofits only

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NLS-tdPCP-GFP-VP16

Plasmid

#183935

Purpose

PCP tandem dimer binding to PP7 RNA stem loops coupled to VP16 activation domain; labeled with GFP

Depositor

Karsten Rippe

Article

Trojanowski et al Mol Cell. 2022 May 4. pii: S1097-2765(22

Insert

tdPCP-GFP-VP16 (UL48 Synthetic)

Tags

NLS

Expression

Mammalian

Mutation

none

Promoter

CMV (TATA box removed)

Available Since

June 13, 2022

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(1)-CRY2-mCherry

Plasmid

#160440

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 1.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-attB2-SA(0)-CRY2-mCherry

Plasmid

#160439

Purpose

To create Cry2-mCherry lines from MiMIC lines with inserts into coding introns in Phase 0.

Depositor

Nicholas Tolwinski

Article

Bunnag et al J Mol Biol. 2020 May 1;432(10):3159-3176

Insert

Cryptochrome 2 (CRY2 Mustard Weed)

Tags

mCherry

Expression

Insect

Available Since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCellFree_G03 AKT1

Plasmid

#67102

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

AKT1 (AKT1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 BMPR1A

Plasmid

#67105

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

BMPR1A (BMPR1A Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 EHD2

Plasmid

#67107

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

EHD2 (EHD2 Human)

Use

Cell-free expression

Tags

EGFP and His

Mutation

S320T, E384D, T387A, H388Q, T405V, V407A, H418R, …

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pCellFree_G03 GTF2H1

Plasmid

#67108

Purpose

Standard for cell-free expression benchmarking.

Depositor

Kirill Alexandrov

Article

Gagoski et al Biotechnol Bioeng. 2015 Aug 24. doi: 10.

Insert

GTF2H1 (GTF2H1 Human)

Use

Cell-free expression

Tags

EGFP and His

Promoter

T7

Available Since

Nov. 6, 2015

Availability

Academic Institutions and Nonprofits only
pRevLCav2FeS

Plasmid

#60808

Purpose

to disrupt iron sulfur cluster in yeast Rev3

Depositor

Youri Pavlov, Tahir Tahirov

Article

Baranovskiy et al J Biol Chem. 2012 May 18;287(21):17281-7

Insert

REV3 (REV3 Budding Yeast)

Expression

Yeast

Mutation

contains 647 C-terminal amino acids and 335 bp of…

Available Since

March 9, 2015

Availability

Academic Institutions and Nonprofits only
pBABEpuro-ARAF_D429A

Plasmid

#51080

Purpose

mammalian expression of ARAF D429A

Depositor

Matthew Meyerson

Article

Imielinski et al J Clin Invest. 2014 Apr;124(4):1582-6. d

Insert

ARAF (ARAF Human)

Use

Retroviral

Expression

Mammalian

Mutation

D429A

Available Since

Feb. 4, 2014

Availability

Academic Institutions and Nonprofits only

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pJK048

Plasmid

#75230

Purpose

Produces Escherichia coli N5-carboxyaminoimidazole ribonucleotide mutase (EcPurE1-H45W) and N5-carboxyaminoimidazole ribonucleotide synthetase (EcPurK)

Depositor

T. J. Kappock, JoAnne Stubbe

Article

Hoskins et al Biochemistry. 2007 Mar 13;46(10):2842-55

Inserts

N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase

Expression

Bacterial

Mutation

Pro64 to Arg; Asp65 to His; Gln206 to Arg and cha…

Promoter

T7 and n/a

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-FLEX-TeLC-P2A-dTomato

Plasmid

#159102

Purpose

Cre-dependent tetanus toxin light chain construct with nuclear-localized dTomato fluorophore for conditional silencing of neurons.

Depositor

Sandeep Datta

Article

Pashkovski et al Nature. 2020 Jul;583(7815):253-258. doi:

Insert

TeLC-P2A-NLS-dTomato

Use

AAV

Promoter

hSynapsin

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-EFS-hfCas13d-HA-mCherry-pA

Plasmid

#233029

Purpose

To Express HA Tagged hfCas13d-mCherry fusion protein from the mammalian EFS promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

hfCas13d-mCherry

Use

AAV

Tags

mCherry

Available Since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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Mfn2-YFP

Plasmid

#28010

Depositor

Richard Youle

Article

Karbowski et al J Cell Biol. 2002 Dec 23. 159(6):931-8.

Insert

mitofusin 2 (MFN2 Human)

Tags

YFP

Expression

Mammalian

Mutation

silent S508S

Available Since

Feb. 24, 2011

Availability

Academic Institutions and Nonprofits only

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pEF5/FRT/DEST-3xHA/mGli2

Plasmid

#51250

Purpose

Encodes N-3xHA-TEV-mouseGli2-C

Depositor

Rajat Rohatgi

Article

Niewiadomski et al Cell Rep. 2013 Dec 24. pii: S2211-1247(1

Insert

Gli2 (Gli2 Mouse)

Use

Flpin system

Tags

3xHA-TEV

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 10, 2014

Availability

Academic Institutions and Nonprofits only
A*11:01:01

Plasmid

#203281

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pFUSE-CHIg-hG1_AT118-L-Fc LALA + PG

Plasmid

#222852

Purpose

Mammalian expression plasmid for secretion of AT118-L a humanized nanobody antagonist of the human Angiotensin II type I receptor (AT1R) fused to human IgG1 Fc

Depositor

Andrew Kruse

Article

Skiba et al Nat Chem Biol. 2024 May 14. doi: 10.1038

Insert

AT118-L

Tags

IgG1_Fc

Expression

Mammalian

Mutation

L234A, L235A, P329G

Available Since

Feb. 10, 2025

Availability

Academic Institutions and Nonprofits only

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B*57:03:01

Plasmid

#203260

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*57:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1 HA-FAM208A

Plasmid

#115845

Purpose

Encodes codon optimized HA tagged FAM208A

Depositor

Jeremy Luban

Article

Yurkovetskiy et al Nat Microbiol. 2018 Dec;3(12):1354-1361.

Insert

HA tagged FAM208A (C3orf63 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD1_1

Plasmid

#160773

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD1_2

Plasmid

#160774

Purpose

Suppress CISD1

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD1_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD2_2

Plasmid

#160776

Purpose

Suppress CISD2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD2_2

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLKO.1P CISD2_1

Plasmid

#160775

Purpose

Suppress CISD2

Depositor

Richard Possemato

Article

Sviderskiy et al Mol Cell. 2020 Oct 28. pii: S1097-2765(2

Insert

shCISD2_1

Use

Lentiviral

Available Since

Oct. 29, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR30(SapI)-CMV-intron-CasRx-pA

Plasmid

#192485

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

CMV

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-Gluc-RMA-IRES-EGFP

Plasmid

#189629

Purpose

Expresses Gluc-RMA and EGFP under the hSyn promoter. Gluc-RMA for monitoring neuronal transduction.

Depositor

Jerzy Szablowski

Article

Lee et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

Inserts

Gaussia luciferase fused to Fc
IRES-EGFP

Use

AAV and Luciferase

Tags

Gluc and IgG1 Fc

Expression

Mammalian

Promoter

IRES and hSyn

Available Since

Nov. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pCDNA-CRY2-mCh-superPLDx100-P2A-CIBN-CAAX

Plasmid

#188994

Purpose

A plasmid for dual expression of CRY2-mCh-superPLDx100 (an engineered PLD mutant with 100 times higher activity than the wild-type) and plasma membrane-tagged CIBN

Depositor

Jeremy Baskin

Article

Tei et al Nat Chem. 2023 May 22. doi: 10.1038/s415

Insert

PLD

Tags

CAAX, CIBN, CRY2, P2A, and mCherry

Expression

Mammalian

Mutation

K57R, A59V, K109R, P245A, V264I, G328S, G381V, G4…

Promoter

CMV

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-DR(RfxCas13d)-CAG-hfCas13d-pa

Plasmid

#233036

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged hfCas13d from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and hfCas13d

Use

AAV

Available Since

March 20, 2025

Availability

Academic Institutions and Nonprofits only

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pMT1-24X-NOG(AtUBI10)

Plasmid

#202016

Purpose

T-DNA vector for expression of the two protein components of the MoonTag system (dCas9-24XGP41 and NbGP41P-sfGFP-VP64-GB1) under the control of the AtUBI10 promoter.

Depositor

Michael Smanski

Article

Casas-Mollano et al Nucleic Acids Res. 2023 Jul 21;51(13):70

Inserts

dCas9-24XGP41
NbGP41P-sfGFP-VP64-GB1

Tags

GB1 and sfGFP

Expression

Plant

Promoter

AtUBI10

Available Since

Aug. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

Plasmid

#233035

Purpose

To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

RfxCas13d-compatible gRNA expression cassette and CasRx

Use

AAV

Available Since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-U6-DR30(SapI)-EFS-CasRx-T2A-mCherry-pA

Plasmid

#192481

Purpose

To express CasRX and a gRNA

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

CasRx

Use

AAV

Mutation

No

Promoter

U6/EFS

Available Since

Aug. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
B*35:03:01

Plasmid

#203255

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

B*35:03:01 (HLA-B Human)

Expression

Mammalian

Available Since

July 7, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
A*24:02:01

Plasmid

#203295

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*24:02:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 10, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pAAV-CMV-intron-hfCas13d-pA

Plasmid

#195864

Purpose

To express hfCas13d

Depositor

Jonathan Ploski

Article

Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

Insert

high fidelity (hf) version of CasRx (hfCas13d)

Use

AAV

Mutation

Yes: GTGGAATACATTACCAACGTGGTGTACGTG

Promoter

CMV

Available Since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-GP-2-KDM4A double Tudor

Plasmid

#59699

Purpose

Contains histone modification interacting domain (KDM4A double Tudor)

Depositor

Albert Jeltsch

Article

Kungulovski et al Genome Res. 2014 Nov;24(11):1842-53. doi

Insert

KDM4A double Tudor (KDM4A Human)

Tags

GST

Expression

Bacterial

Promoter

tac promoter

Available Since

Oct. 17, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
LENTICRISPR-UPP1_sgRNA1

Plasmid

#201634

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

UPP1 (UPP1 Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
PB-CAG-DDdCas9VPH-T2A-GFP-IRES-Neo

Plasmid

#102886

Purpose

PiggyBac transposon system construct with constitutive CAG promoter expression of TMP inducible DDdCas9VP192-p65-HSF1 activator followed by T2A-EGFP as a reporter and IRES-Neo as selection

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

DDdCas9VP192-p65-HSF1-T2A-GFP-IRES-Neo

Use

CRISPR

Expression

Mammalian

Mutation

D10A, H840A

Promoter

CAG

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
A*11:01:01:01

Plasmid

#203276

Purpose

Overexpress HLA allele

Depositor

Derin Keskin, Catherine Wu

Article

Sarkizova et al Nat Biotechnol. 2020 Feb;38(2):199-209.

Insert

A*11:01:01:01 (HLA-A Human)

Expression

Mammalian

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pDsRed1-CMV-Rat Arc-DsRed1-pA

Plasmid

#233049

Purpose

To Express a Rat Arc-DsRed fusion protein from a CMV promoter

Depositor

Jonathan Ploski

Article

de Solis et al Neurobiol Learn Mem. 2015 Oct;124:34-47.

Insert

rat Arc-DsRed fusion (Arc Rat)

Tags

DsRed1

Expression

Mammalian

Available Since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 7,580 results for: Ski;