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We narrowed to 8,805 results for: ef1;

Showing: 1851 - 1900 of 8805 results

  3. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

    Plasmid
    #227246
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227246-plasmid-map-sequence-id-450569

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  10. pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

    Plasmid
    #234953
    Purpose
    Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid
    Depositor
    Agnel Sfeir
    Article
    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.
    Insert
    human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    Use
    Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    April 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    234953-plasmid-map-sequence-id-467731

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  12. EF1α-Lox2272-LoxP-MARKS·mTurquoise2-NLS·turboRFP-Lox2272-LoxP

    Plasmid
    #198753
    Purpose
    Cre reporter, which switches from membraneous mTurquoise to nuclear RFP upon Cre recombinase activity
    Depositor
    Jacco van Rheenen
    Article
    Vizoso et al Nat Commun. 2022 Oct 28;13(1):6442. doi:
    Insert
    EF1a promotor-Lox2272-LoxP-MARKS-mTurquoise2-NLS.turboRFP-Lox2272-LOXP
    Use
    Lentiviral
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    See depositor comments below
    Promoter
    EF1a
    Available since
    July 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    198753-plasmid-map-sequence-id-398000

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  24. AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato

    Plasmid
    #51082
    Purpose
    Cre dependent expression of GCaMP6s Ca sensor and physically separate nuclear localized dTomato fluorophore
    Depositor
    Jonathan Ting
    Has Service
    AAV1
    Insert
    GCaMP6s
    Use
    AAV
    Tags
    P2A-nls-dTomato
    Expression
    Mammalian
    Mutation
    Promoter
    hEF1a
    Available since
    Feb. 27, 2014
    Availability
    Academic Institutions and Nonprofits only
    51082-plasmid-map-sequence-id-175187

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  25. pOTTC1032 - pAAV EF1a Nuc-flox(mCherry)-EGFP

    Plasmid
    #112677
    Purpose
    An AAV vector that expresses a Cre-dependent nuclear-localized Red to Green Fluorescent protein
    Depositor
    Brandon Harvey
    Article
    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3
    Has Service
    AAV Retrograde, AAV1, AAV2, AAV5, AAV8, and AAV9
    Insert
    Nuclear-localized floxed-mCherry EGFP
    Use
    AAV
    Tags
    Nuclear localization signal
    Expression
    Mutation
    Promoter
    EF1a
    Available since
    March 7, 2019
    Availability
    Academic Institutions and Nonprofits only
    112677-plasmid-map-sequence-id-237606

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  26. PB513B-1/TRE-hHNF1β-hHNF4α-hHNF6-EF1α-Bla

    Plasmid
    #199551
    Purpose
    PiggyBac-based transposon vector plasmid which encodes the expression units of liver-enriched transcription factor genes (hHNF1β-hHNF4α-hHNF6) under control of the TRE/PCMVmin promoter
    Depositor
    Masamichi Kamihira
    Article
    Kitano et al Cells. 2022 Apr 1;11(7):1194. doi: 10.33
    Insert
    hHNF1β-hHNF4α-hHNF6 (ONECUT1, HNF4A, HNF1B Human)
    Use
    Piggybac transposon vector
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    TRE+CMVmin promoter
    Available since
    Aug. 24, 2023
    Availability
    Academic Institutions and Nonprofits only
    199551-plasmid-map-sequence-id-405450

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  35. pCDH-EF1-DIO-Nluc-copGFP-IRES-Puro

    Plasmid
    #192875
    Purpose
    Cre-dependent dual reporter
    Depositor
    Kazuhiro Oka
    Insert
    Nanoluc-P2A-copGFP
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Dec. 5, 2022
    Availability
    Academic Institutions and Nonprofits only
    192875-plasmid-map-sequence-id-378875

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  36. EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [C12orf35]

    Plasmid
    #227245
    Purpose
    All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in CHO cell
    Depositor
    Jae Seong Lee
    Article
    Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.
    Inserts
    Tet-On 3G transactivator protein
    Enhanced green fluorescent protein
    Use
    Cre/Lox and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1α and pTRE3G
    Available since
    Oct. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    227245-plasmid-map-sequence-id-450568

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  39. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid
    #99277
    Purpose
    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance
    Depositor
    Jordan Miller
    Article
    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/
    Inserts
    H2B-mOrange2-IRES-EGFP
    Puro
    Use
    Lentiviral
    Tags
    EGFP, H2B, IRES, PGK, and mOrange2
    Expression
    Mutation
    Promoter
    EF1 alpha-human and PGK-mouse
    Available since
    Nov. 13, 2017
    Availability
    Academic Institutions and Nonprofits only
    99277-plasmid-map-sequence-id-197594

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  40. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA

    Plasmid
    #134925
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134925-plasmid-map-sequence-id-267605

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  42. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA

    Plasmid
    #134922
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134922-plasmid-map-sequence-id-267588

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  43. pAAV-EF1α-DIO-GtACR2-P2A-Voltron2ST-W3SL

    Plasmid
    #217676
    Purpose
    AAV-mediated, Cre-dependent co-expression of GtACR2 and soma-targeted Voltron2 (ORCHID) for assessing inhibitory receptor driving force through voltage imaging with concurrent activation of GtACR2.
    Depositor
    Joseph Raimondo
    Insert
    GtACR2-P2A-Voltron2ST
    Use
    AAV
    Tags
    Soma targeting sequence (Kv2.1) on Voltron2 gene …
    Expression
    Mammalian
    Mutation
    Promoter
    EF‐1α
    Available since
    May 6, 2024
    Availability
    Academic Institutions and Nonprofits only
    217676-plasmid-map-sequence-id-432468

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  44. pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iPA

    Plasmid
    #134924
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iPA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses PuroR protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse1(Cas8), Cas5,Cas7) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134924-plasmid-map-sequence-id-267600

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  45. pLVX-EF1α-BHLHE40 aa1-297-IRES-ZsGreen1

    Plasmid
    #153062
    Purpose
    Lentiviral vector expressing a truncated form of human BHLHE40 (aa 1-297)
    Depositor
    Silvia Monticelli
    Article
    Emming et al Nat Immunol. 2020 Apr;21(4):388-399. doi
    Insert
    Truncated form of human BHLHE40 (aa 1-297) (BHLHE40 Human)
    Use
    Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Mutagenesis to insert a stop codon at aa 298 in B…
    Promoter
    EF1α
    Available since
    July 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153062-plasmid-map-sequence-id-304636

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  46. pAAV-Ef1a-vCreDIO hChR2(H134R)-eYFP 2.0

    Plasmid
    #126081
    Purpose
    vCre-Dependent ChR2(H134R)-eYFP with the vLox sites in the reverse orientation
    Depositor
    Karl Deisseroth
    Insert
    hChR2(H134R)-eYFP
    Use
    AAV
    Tags
    eYFP
    Expression
    Mutation
    H134R
    Promoter
    Ef1a
    Available since
    May 22, 2019
    Availability
    Academic Institutions and Nonprofits only
    126081-plasmid-map-sequence-id-245074

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  48. pPV-EF1a-2xNLS-Cse2-Cas6-Cas3-iCA

    Plasmid
    #134923
    Purpose
    Components for genome editing in mammalian cells with pPV-EF1a-2xNLS-Cas7-Cas5-Cse1-iCA and pBS-U6-crRNA-targeted. This plasmid was optimized for iPS cells and coexpresses mCherry protein.
    Depositor
    Tomoji Mashimo
    Article
    Morisaka et al Nat Commun. 2019 Dec 6;10(1):5302. doi:
    Insert
    Cascade factors (Cse2(Cas11), Cas3, Cas6) with bpNLS
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EF1a
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    134923-plasmid-map-sequence-id-267593

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Showing: 1851 - 1900 of 8805 results