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pFastbac-Dual-StrepII-BARD1

Plasmid

#137166

Purpose

Expresses human StrepII-tagged BARD1 for purification from baculovirus/insect cell system

Depositor

Andrew Deans

Article

Tan et al PLoS One. 2020 Feb 24;15(2):e0229000. do

Insert

BARD1 (BARD1 Human)

Use

Tags

StrepII

Expression

Insect

Mutation

fully synthetic, codon optimized for insect expre…

Promoter

p10

Available since

April 15, 2020

Availability

Academic Institutions and Nonprofits only

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pTwist-SARS-CoV-2 Spike-deltaC18 HK.3

Plasmid

#212572

Purpose

Encodes SARS-CoV-2 spike for HK.3 pseudovirus production

Depositor

Alejandro Balazs

Article

Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

Insert

SARS-CoV-2 Spike HK.3 (S Severe acute respiratory syndrome coronavirus 2)

Use

Tags

Expression

Mammalian

Mutation

See Depositor Comments.

Promoter

CAG

Available since

Sept. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pcdna 3.1 DSG-3 Tension Sensor

Plasmid

#182717

Purpose

expresses DSG-3 TSmod

Depositor

Daniel Conway

Article

Jin et al bioRxiv [Preprint]. 2024 May 6:2024.05.0

Insert

desmoglein-3 (Dsg3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

TSmod inserted between G726 and G727

Promoter

Available since

July 22, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID-dPB1-SQSTM1

Plasmid

#223716

Purpose

Expresses FLAG-TurboID-dPB1-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 1-119

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-FLAG-TurboID-dUBA-SQSTM1

Plasmid

#223717

Purpose

Expresses FLAG-TurboID-dUBA-SQSTM1

Depositor

Benjamin Wolozin

Article

Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

Insert

SQSTM1 (SQSTM1 Human)

Use

Lentiviral

Tags

TurboID

Expression

Mammalian

Mutation

deletion 384-Stop codon

Promoter

SFFV

Available since

Aug. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pStreptagIIx2 Alfatag 3C InsR EABR

Plasmid

#234996

Purpose

For production of Extracellular Vesicles (EVs), with the Insulin Receptor Strep-tag II labeled on their surface

Depositor

Monica Gonzalez-Magaldi, Daniel Leahy

Article

Gonzalez-Magaldi et al bioRxiv [Preprint]. 2024 Nov 28:2024.11.

Insert

InsR-EABR (INSR Human)

Use

Tags

2xStreptag-II, Alfatag, HRV 3C site

Expression

Mammalian

Mutation

Promoter

Available since

April 22, 2025

Availability

Academic Institutions and Nonprofits only

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A1-LCD allW

Plasmid

#234609

Purpose

Bacterial expression of N-terminally 6His tagged hnRNPA1 LCD (186-320) with all aromatic amino acids mutated to W keeping the hexapeptide fibril core (SYNDFG) intact.

Depositor

Tanja Mittag

Article

Das et al bioRxiv [Preprint]. 2024 Mar 3:2024.02.2

Insert

hnRNPA1_LCD_allW (HNRNPA1 Human)

Use

Tags

6xHis-TEV

Expression

Bacterial

Mutation

C-terminal domain (186-320) with all aromatic ami…

Promoter

T7

Available since

April 15, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-E1MLS-2xFLAG-T4Lig-IRES-Neo

Plasmid

#234953

Purpose

Human codon optimized T4 DNA Ligase with N-terminal E1 MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-EF1a-FOATPMLS-2xFLAG-T4Lig-IRES-Neo

Plasmid

#234954

Purpose

Human codon optimized T4 DNA Ligase with N-terminal F0ATP MLS expressing plasmid

Depositor

Agnel Sfeir

Article

Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

Insert

human codon optimized T4 DNA Ligase with N-terminal mitochondrial localization signal of subunit 9 of F0-ATP synthase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

April 3, 2025

Availability

Academic Institutions and Nonprofits only

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pGenlenti_ATG13-delta191-230

Plasmid

#223772

Purpose

Stable expression of protein in mammalian cell culture, rescue experiments

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Lentiviral

Tags

Expression

Mutation

aa 191-230 deleted (expresses 1-190, 231-517)

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGenlenti_ATG13-delta191-205

Plasmid

#223773

Purpose

Stable expression of protein in mammalian cell culture, rescue experiments

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Lentiviral

Tags

Expression

Mutation

aa 191-205 deleted (expresses 1-190, 206-517)

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGenLenti V5-NIX (W36A/L39A; deltaLIR)

Plasmid

#223788

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

W36A/L39A; deletion of LIR

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pCAG_mCherry-ATG13 HORMA (1-191aa)

Plasmid

#223759

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 HORMA (ATG13 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

aa 1-191 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pGEX4T1_GST-EGFP-ATG13_IDR (191-517aa)

Plasmid

#223760

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 IDR (ATG13 Human)

Use

Tags

GST-EGFP

Expression

Bacterial

Mutation

aa 191-517 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-THROMBIN-GST

Plasmid

#223764

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 soluble part (BNIP3 Human)

Use

Tags

Thrombin cleavage-GST

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pFastBac-Dual_BNIP3(1-158aa)-EGFP-TEV-GST

Plasmid

#223765

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 soluble part (BNIP3 Human)

Use

Tags

EGFP-TEV-GST

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-FKBP-mEGFP-WIPI1

Plasmid

#223767

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI1 (WIPI1 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_NIX(1-182aa)-EGFP-TEV-GST (WT)

Plasmid

#223736

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pETDuet1_FUNDC1(1-50aa)-EGFP-TEV-GST (WT)

Plasmid

#223737

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) soluble part (BNIP3L Human)

Use

Tags

EGFP-TEV-GST

Expression

Bacterial

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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pBIG1a_GST-TEV-CK2alpha_CK2beta

Plasmid

#223740

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

CK2-alpha, CK2-beta (CSNK2A1 Human)

Use

Tags

GST-TEV

Expression

Insect

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

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