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pTriEX Tom20 mVenus BoPL

Plasmid

#114467

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoPL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoPL

Use

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEx TOM20 mVenus BoLL

Plasmid

#114465

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLL

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLL

Use

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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pTriEX Tom20 mVenus BoLD

Plasmid

#114468

Purpose

Mammalian expression of mitochondrially targeted, mVenus labelled BoLD

Depositor

Andrew Woolley

Article

Reis et al ACS Synth Biol. 2018 Oct 19;7(10):2355-2

Insert

BoLD

Use

Tags

HIS, TEV and FLAG and TOM20 and mVenus

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 25, 2018

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-chimpTRIM5-FOS

Plasmid

#79065

Purpose

Express chimpanzee TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(chimpanzee)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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CSII-IDR2-AGMpygTRIM5-FOS

Plasmid

#79064

Purpose

Express African green monkey pygerythrus TRIM5alpha in mammalan cells

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

TRIM5alpha(African green monkey pygerythrus)-FOS

Use

Tags

OneSTrEP-FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 20, 2017

Availability

Academic Institutions and Nonprofits only

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pTRIPZ (M)-HT-Cbx7-Delta-ATL

Plasmid

#82523

Purpose

Lentivirus vector. Expresses mutant Halotag-Cbx7-delta-ATL fusion proteins in mammalian cells. Cbx7deletion of amino acids 66–83.

Depositor

Xiaojun Ren

Insert

Chromobox Homolog 7 (CBX7 Human)

Use

Lentiviral

Tags

HaloTag

Expression

Mammalian

Mutation

deletion of amino acids 66–83

Promoter

CMV

Available since

Dec. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pFastBac1-Kozak-rhTRIM5alpha-PFOS

Plasmid

#79038

Purpose

Baculoviral entry vector to produce Bac-to-bac baculoviruses

Depositor

Wesley Sundquist

Article

Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

Insert

rhesus TRIM5alha-PFOS

Use

Tags

OneSTrEP-FLAG

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pKLBac-Bat-FluA-trimer

Plasmid

#229858

Purpose

Influenza A/little yellow-shouldered bat/Guatemala/060/2010(H17N10) polymerase heterotrimer (self-cleaving polyprotein)

Depositor

Stephen Cusack

Article

Pflug et al Nature. 2014 Dec 18;516(7531):355-60. do

Inserts

PA_Bat-FluA_AFC35437.1
PB1_Bat-FluA_AFC35436
PB2_Bat-FluA_AFC35435.1

Use

Tags

Expression

Insect

Mutation

Promoter

Available since

Feb. 25, 2025

Availability

Academic Institutions and Nonprofits only

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Anti-Beta4-spectrin [N393/2R-2b]

Plasmid

#222173

Purpose

Mammalian Expression Plasmid of anti-Beta4-spectrin (Human). Derived from hybridoma N393/2.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

Anti-Beta4-spectrin (Homo sapiens) recombinant mouse monoclonal antibody. (SPTBN4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Dual CMV

Available since

Sept. 5, 2024

Availability

Academic Institutions and Nonprofits only

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LMPd Ametrine Got1 shRNA#1

Plasmid

#220592

Purpose

Retroviral vector with Ametrine marker for expression shRNA with an "UltramiR" microRNA scaffold

Depositor

Russell Jones

Article

Ma et al bioRxiv [Preprint]. 2023 Jun 11:2023.06.

Insert

Got1 shRNA (Got1 Mouse)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Aug. 13, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-FKBP-TRIF(Nterm)

Plasmid

#221334

Purpose

Overexpression of TRIF Nterm domain in bacteria

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF Nterm domain (Ticam1 Mouse)

Use

Tags

6xHis-4xFKBP

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-43)

Plasmid

#221248

Purpose

Expression of TRIF truncation 2 (44-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 2 (44-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-63)

Plasmid

#221249

Purpose

Expression of TRIF truncation 3 (64-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 3 (64-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-83)

Plasmid

#221250

Purpose

Expression of TRIF truncation 4 (84-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 4 (84-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-TRIF(trunc 1-23)

Plasmid

#221247

Purpose

Expression of TRIF truncation 1 (24-222) in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Article

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

TRIF truncation 1 (24-222) (Ticam1 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Expression

Mutation

Promoter

Available since

July 10, 2024

Availability

Academic Institutions and Nonprofits only

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pJT309 RapInducible1-ActivatorCloneSite-Citrine

Plasmid

#187321

Purpose

Rapamycin inducible genetic circuit with cloning site for activator domains

Depositor

Lacra Bintu, Michael Bassik

Article

Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-GFP-2A-Puro-gATRIP_A

Plasmid

#211537

Purpose

sgRNA-A against ATRIP

Depositor

Agnel Sfeir

Article

Brambati et al Science. 2023 Aug 11;381(6658):653-660.

Insert

sgRNA ATRIP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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pTRIPZ-NUDT18-C-FLAG-Puro

Plasmid

#195181

Purpose

Lentiviral vector for DOX-inducible NUDT18 gene expression with C-terminal FLAG tag. Gateway expression system. Selection by puromycin.

Depositor

Stephanie Roessler

Insert

NUDT18 (NUDT18 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Mutation

Promoter

minimal CMV

Available since

Feb. 20, 2024

Availability

Academic Institutions and Nonprofits only

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TRIP8b (exon 4) scFv [N212/3]

Plasmid

#211607

Purpose

Mammalian Expression Plasmid of TRIP8b (exon 4) scFv. Derived from hybridoma N212/3.

Depositor

James Trimmer

Article

Mitchell et al Sci Rep. 2023 Sep 27;13(1):16200. doi: 1

Insert

TRIP8b (exon 4) (Rattus norvegicus) recombinant mouse scFv. (Pex5l Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pKLV2-U6gRNA5(TRIM24_4)-PGKpuroBFP-W

Plasmid

#211988

Purpose

Express gRNA against TRIM24 with puro and BFP

Depositor

Kosuke Yusa

Article

Ishikawa et al Commun Biol. 2023 Dec 28;6(1):1290. doi:

Insert

sgRNA targeting TRIM24 (TRIM24 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Human U6 promoter

Available since

Jan. 24, 2024

Availability

Academic Institutions and Nonprofits only

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