We narrowed to 1,446 results for: phr

1. pHR: pmU6 sgChr13q34 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174119

   Purpose

   Expression of sgRNA targeting Chr13q34 (chr13: 112277011 - 112319170, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr13q34 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pSLQ9713: pHR-PGK-SV40_NLS-dCasMINI-V4-QNZF-c-Myc_NLS-mCherry-WPRE

   Plasmid

   #203219

   Purpose

   Expression of dCasMINI-V4-QNZF-mCherry in mammalian cells

   Depositor

   Stanley Qi

   Article

   Tycko et al Nat Biotechnol. 2024 Nov 1. doi: 10.1038

   Insert

   dCasMINI-V4-QNZF-mCherry
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHR: pmU6 sgChr3q29 2xMS2 pUbC MCP-mCherry-p2a-Puro

   Plasmid

   #174118

   Purpose

   Expression of sgRNA targeting Chr3q29 (chr3: 195478317 - 195506985, hg38)

   Depositor

   Stanley Qi

   Article

   Gao et al Mol Cell. 2021 Aug 13. pii: S1097-2765(2

   Insert

   sgChr3q29 2xMS2 and MCP-mCherry-p2a-Puro
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   mU6 and UbC

   Available Since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pSLQ9834_sgBB: pHR-hU6-CasMINI sgRNA_#2; EF1a-Puro-T2A-BFP- WPRE

   Plasmid

   #180280

   Purpose

   The CasMINI sgRNA cloning backbone with sites 'BsmBI' for inserting new guide sequences.

   Depositor

   Stanley Qi

   Article

   Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

   Insert

   CasMINI sgRNA (backbone) and BFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   March 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ4428 pHR (EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE)

   Plasmid

   #214885

   Purpose

   Lentiviral vector encoding TMP-regulatable RfxCas13d-DD fusion

   Depositor

   Stanley Qi

   Article

   Tieu et al Cell. 2024 Feb 29;187(5):1278-1295.e20.

   Insert

   EF1a-mCherry-P2A-RfxCas13d-2xNLS-ecDHFR-3xFLAG-WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   April 4, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pSLQ8453 pHR-hU6-LbCpf1 crRNA BB-EF1a-Puro-t2a-BFP-WPRE

   Plasmid

   #140086

   Purpose

   Backbone for cloning U6 driven LbCpf1 (Cas12a) crRNA, EF1a driven BFP + puromycin resistance

   Depositor

   Stanley Qi

   Article

   Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

   Insert

   LbCpf1 (LbCas12a) crRNA backbone
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-BFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   May 7, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Emi1 5' and 3'UTR

   Plasmid

   #67930

   Purpose

   Translational reporter - Emi1 UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ control UTRs

   Plasmid

   #67929

   Purpose

   Translational reporter - control UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ ARHGAP5 5' and 3'UTR

   Plasmid

   #67931

   Purpose

   Translational reporter - ARHGAP5 UTRs

   Depositor

   Ron Vale

   Article

   Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

   Insert

   sfGFP-NLS-P2A-NLS-mCherry (ARHGAP5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pHR-UCOE-SFFV-SID4x-dCas9-XTEN80-KRAB(Kox1)-P2A-EGFP

    Plasmid

    #188901

    Purpose

    dCas9 with an N-term Sid4x fusion, a C-term HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, and GFP linked by a P2A site from a SFFV promoter with an upstream ubiquitous chromatin opening element

    Depositor

    Jonathan Weissman, Marco Jost

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    SID-dCas9-XTEN80-KRAB(Kox1)
    

    Use

    Lentiviral

    Tags

    HA-2xNLS-XTEN80(linker)-KRAB(Kox1) fusion, P2A-GF…

    Expression

    Mutation

    Promoter

    SFFV

    Available Since

    Oct. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pHR-DHFRY100I-sfGFP-NLS-P2A-NLS-mCherry-P2A_ Lox 5'UTR

    Plasmid

    #67932

    Purpose

    Translational reporter - Lox 5'UTR

    Depositor

    Ron Vale

    Article

    Tanenbaum et al Elife. 2015 Aug 25;4. doi: 10.7554/eLife

    Insert

    sfGFP-NLS-P2A-NLS-mCherry (LOX Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Sept. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176272

    Purpose

    Expression of non-targeting sgRNA of CasMINI

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI non-targeting sgRNA (sgLacZ) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSLQ9834_sgTet: pHR-hU6-CasMINI sgRNA_#2 (sgTet); EF1a-Puro-T2A-BFP- WPRE

    Plasmid

    #176273

    Purpose

    Expression of CasMINI sgRNA targeting TRE3G promoter in dCasMINI-mediated GFP activation assays.

    Depositor

    Stanley Qi

    Article

    Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

    Insert

    CasMINI sgRNA (sgTet) and BFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    Oct. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSLQ8703 pHR-R(TRE3G-Fluc-2XcrIFNg-SV40pA)-EF1a-sfGFP-T2A-rtta-WPRE

    Plasmid

    #140230

    Purpose

    Dox inducible expression of luciferase + crIFNg. Constitutive EF1a driven rtTA and GFP

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    Luciferase + crIFNg (reverse orientation)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSLQ8666 pHR-RRM2p-BFP-T2A-N term (1-406) dLbCpf1-EE12RR345L-EFS-Zeo-WPRE

    Plasmid

    #140228

    Purpose

    RRM2p-driven expression of BFP and N terminal dCpf1 (Cas12a) 406-split half with leucine zipper. Constitutive EFS-driven zeocin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    dLbCpf1 (Cas12a) N terminal half (406) with zipper
    

    Use

    Lentiviral

    Tags

    BFP

    Expression

    Mammalian

    Mutation

    Promoter

    RRM2 endogenous promoter

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSLQ8629 pHR-HREYBTATA-RR12EE345L-C term (406) dLbCpf1-2XNLS-miniVPR-mCherry-EFS-Puro-WPRE

    Plasmid

    #140227

    Purpose

    Hypoxia-inducible expression of leucine zipper with C terminal dCpf1 (Cas12a) 406-split half fused to miniVPR. Constitutive EFS-driven puromycin resistance.

    Depositor

    Stanley Qi

    Article

    Kempton et al Mol Cell. 2020 Jan 30. pii: S1097-2765(2

    Insert

    zipper with dLbCpf1 (Cas12a) C terminal half (406) fused to miniVPR
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    HRE (Hypoxia inducible)

    Available Since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pSLQ11438

    Plasmid

    #183204

    Purpose

    pHR-EF1a-ABE8e-hyperdLbCas12a-NLS-mCherry-P2A-Puro-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    hyperdCas12 base editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSLQ11353

    Plasmid

    #183098

    Purpose

    pHR-CAG-sfGFP* ABE_3-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    GFP*
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pSLQ11440

    Plasmid

    #183203

    Purpose

    pHR-TRE3G-ABE8e-hyperdLbCas12a-NLS-P2A-mCherry-EFS-Puro-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    hyperdCas12 base editor
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSLQ11429

    Plasmid

    #183205

    Purpose

    pHR-R(NFKB-BFP-Triplex-2X crGFP*ABE3-SV40pA)-EFS-Hygro-T2A-rtTA-WPRE

    Depositor

    Stanley Qi

    Article

    Kempton et al Nat Chem Biol. 2022 May 30. pii: 10.1038

    Insert

    Cas12 Guide (crGFP*)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge