We narrowed to 5,093 results for: puc

1. pUC19_msfGFP-EWSR1-repair-tempate

   Plasmid

   #237682

   Purpose

   For knock-in msfGFP to EWSR1 locus

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   EWSR1 (EWSR1 Human)
   

   Use

   CRISPR

   Tags

   msfGFP

   Available Since

   May 6, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pUC18-mini-Tn7T-Gm-LacZ10

   Plasmid

   #65026

   Purpose

   GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 1. See Depositor Comments below for information regarding the lacZ sequence.

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Type

   Empty backbone

   Tags

   lacZ

   Expression

   Bacterial

   Available Since

   Nov. 2, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pUC8 mouse IFN-gamma

   Plasmid

   #10955

   Depositor

   Howard Young

   Insert

   interferon-gamma (Ifng Mouse)
   

   Use

   Cloning vector

   Available Since

   Feb. 14, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pUC19-link-sfGFP-FLAG:Kan

   Plasmid

   #211833

   Purpose

   Plasmid template that encodes the fluorescent protein sfGFP with a FLAG tag next to kanamycin resistance gene. This template was used to copy out sfGFP-FLAG-Kan for genetic tagging.

   Depositor

   Peng Chen

   Insert

   sfGFP-FLAG-Kan
   

   Expression

   Bacterial

   Mutation

   NA

   Available Since

   Jan. 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUC18T-mini-Tn7T-Tp-eyfp

   Plasmid

   #65035

   Purpose

   TpR mini-Tn7 vector, mobilizable, for YFP tagging bacteria

   Depositor

   Herbert Schweizer

   Article

   Choi et al Nat Protoc. 2006;1(1):153-61.

   Insert

   eyfp
   

   Expression

   Bacterial

   Available Since

   Aug. 14, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC-UAS1B16-TEF(504)

   Plasmid

   #44371

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(504) promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(504)

   Available Since

   July 15, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pUC-UAS1B16-TEF(272)

   Plasmid

   #44375

   Depositor

   Hal Alper

   Article

   Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

   Insert

   UAS1B16-TEF(272) promoter
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Mutation

   N/A

   Promoter

   UAS1B16-TEF(272)

   Available Since

   April 18, 2013

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair3

   Plasmid

   #98974

   Purpose

   Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 37bp 3' overhangs. TagBFP.

   Depositor

   Jan Karlseder

   Article

   Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

   Insert

   LMNA sgRNAs pair 3 and Cas9(D10A)
   

   Expression

   Mammalian

   Available Since

   Nov. 29, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pUC-dirTopo-P2A-aGFP

   Plasmid

   #61974

   Purpose

   A directional cloning vector of PCR-amplified cDNAs for mammalian expression

   Depositor

   Hiroshi Udo

   Inserts

   lacZ alpha
   EGFP
   

   Expression

   Mammalian

   Mutation

   It contains silent mutations

   Available Since

   Feb. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUC-UAS1B8-TEF(136)

    Plasmid

    #44380

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(136) promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(136)

    Available Since

    April 17, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. pUC-dirTopo-AU1-aGFP

    Plasmid

    #61973

    Purpose

    A directional cloning vector of PCR-amplified cDNAs for mammalian expression

    Depositor

    Hiroshi Udo

    Inserts

    lacZ alpha
    EGFP
    

    Expression

    Mammalian

    Mutation

    It contains silent mutations

    Available Since

    Feb. 4, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pUC57-Rluc-A114-N40

    Plasmid

    #50561

    Purpose

    Template DNA for in vitro transcription using T7 polymerase

    Depositor

    Yukihide Tomari

    Article

    Fukaya et al EMBO J. 2011 Nov 25;30(24):4998-5009. do

    Insert

    Luciferase-A114-N40
    

    Use

    Cloning vector

    Promoter

    lac

    Available Since

    Feb. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUC-UAS1B8-TEF(272)

    Plasmid

    #44376

    Depositor

    Hal Alper

    Article

    Blazeck et al Appl Environ Microbiol. 2011 Nov;77(22):

    Insert

    UAS1B8-TEF(272) promoter
    

    Use

    Synthetic Biology

    Expression

    Bacterial

    Mutation

    N/A

    Promoter

    UAS1B8-TEF(272)

    Available Since

    April 16, 2013

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pUC57-codon-modified HTK

    Plasmid

    #34563

    Depositor

    David Anderson

    Article

    Lo et al Neuron. 2011 Dec 22;72(6):938-50.

    Insert

    codon-modified HTK
    

    Expression

    Bacterial

    Mutation

    codon modified HTK using mouse codon usage table

    Promoter

    pUC57 bacteria promoter

    Available Since

    Jan. 9, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC CBA-SpCas9D10Anickase.EF1a-BFP.sgLMNApair4

    Plasmid

    #98975

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9D10A nickase and a pair of sgRNAs targeting hLMNA. Generates breaks with 94bp 3' overhangs. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNAs pair 4 and Cas9(D10A)
    

    Expression

    Mammalian

    Available Since

    Nov. 29, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC-hU6-crScaffold_SV40-BFP

    Plasmid

    #224860

    Purpose

    crRNA scaffold for RfxCas13d expressed from hU6 promoter and reporter BFP protein expressed from SV40 promoter

    Depositor

    Gabor Balazsi

    Article

    Wan et al ACS Synth Biol. 2024 Oct 18;13(10):3212-

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    hU6

    Available Since

    Oct. 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pUC19-link-mVenus-FLAG:Kan

    Plasmid

    #211830

    Purpose

    Plasmid template that encodes the fluorescent protein mVenus with a FLAG tag next to kanamycin resistance gene. This template was used to copy out mVenus-FLAG-Kan for genetic tagging.

    Depositor

    Peng Chen

    Insert

    mVenus-FLAG-Kan
    

    Expression

    Bacterial

    Mutation

    NA

    Available Since

    Jan. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pUC18-mini-Tn7T-Gm-LacZ20

    Plasmid

    #65027

    Purpose

    GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.

    Depositor

    Herbert Schweizer

    Article

    Choi et al Nat Protoc. 2006;1(1):153-61.

    Type

    Empty backbone

    Tags

    lacZ

    Expression

    Bacterial

    Available Since

    Nov. 2, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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19. pUC19.TRIM28.FKBP.knock-in.mRuby2

    Plasmid

    #185008

    Purpose

    For knocking in FKBP F36V P2A mRuby2

    Depositor

    Denes Hnisz

    Article

    Asimi et al Nat Genet. 2022 Aug;54(8):1238-1247. doi

    Insert

    FKBP F36V P2A mRuby2
    

    Use

    Cloning

    Available Since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUC18T-mini-Tn7T-Gm-Pc-mKate2

    Plasmid

    #199249

    Purpose

    Fluorescent labelling of bacterial cells with mKate2

    Depositor

    Uwe Mamat

    Article

    Mamat et al Appl Environ Microbiol. 2023 May 17:e003

    Insert

    Codon-optimized mKate2 gene
    

    Expression

    Bacterial

    Mutation

    Codon optimization for Xanthomonadaceae

    Promoter

    Pc promoter from class III integron of Delftia a…

    Available Since

    May 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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