We narrowed to 13,692 results for: Egf
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Plasmid#194937PurposeLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
PKC1-2GFP-ter-TRP1
Plasmid#184773PurposeIntegration of 2xGFP tag at PKC1 C terminus. Uses auxotrophic marker TRP1(Kluyveromyces lactis).DepositorInsertPKC1 (PKC1 Budding Yeast)
UseTags2EGFPExpressionYeastMutationPromoterAvailable sinceJan. 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
Full-length WT talin1
Plasmid#182992Purposeexpress FL WT talin1 in mammalian cellsDepositorInsertTalin1 WT (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
Full-length P229S talin1
Plasmid#182993Purposeexpress FL variant P229S talin1 in mammalian cellsDepositorInsertTalin1 P229S (Tln1 Mouse)
UseTagsGFPExpressionMammalianMutationP229SPromoterAvailable sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-preSufI(IAC)
Plasmid#168516PurposeModified form of pre-SufI in pET25b. With C-terminal HHHHHHC (6xHisC) extension and mutations C17I and C295A.DepositorInsertpre-SufI(IAC)
UseTags6xHisC tag and HSV tagExpressionBacterialMutationC17I and C295APromoterT7Available sinceSept. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP4D42
Plasmid#185840PurposeTesting the TEF1 promoter inserted with 4 Z268 elements using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1+[Z268]>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 6, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF71
Plasmid#185860PurposeTesting the SkGAL2 promoter inserted with 4 Z268 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2+[Z268]>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72 (B9)
Plasmid#185858PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 element using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M5(5*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10CF72
Plasmid#185857PurposeTesting the SkGAL2 promoter inserted with 4 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M4(4*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1B
Plasmid#185856PurposeTesting the SkGAL2 promoter inserted with 3 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M2(3*PZ4)>(BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP10E1A
Plasmid#185855PurposeTesting the SkGAL2 promoter inserted with 2 PZ4 elements using a green fluorescent protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1- PSkGAL2M1(2*PZ4)> (BamHI)yEGFP>TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pILGFP3A2 (1)
Plasmid#185851PurposeTesting the TEF1 promoter appended with 2 tetracycline riboswitch using a green fluorescence protein as the reporterDepositorInsertPURA3>KlURA3>TAgTEF1-PTEF1>2*TcRb>yEGFP>TPGK1-TURA3
UseTagsExpressionYeastMutationNonePromoterAvailable sinceAug. 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET15 PemrR-gfp biosensor
Plasmid#185793PurposeThe pET15 PemrR-gfp biosensor is transcriptional fusion of GFP with the promoter of the emrRAB operon. This biosensor is responsive to monoaromatics, such as vanillin and syringaldehyde.DepositorInsertpEmrR-gfp
UseTagsEGFPExpressionBacterialMutationPromoterPemrRAvailable sinceAug. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGPN
Plasmid#187482PurposeExpression vector - GFPDepositorInsertEGFP
UseLentiviralTagsExpressionMutationPromoterEFSAvailable sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-mouse-Caspase-1-H340D
Plasmid#183362PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1 H340D (Casp1 Mouse)
UseRetroviralTagsMycExpressionMutationH340DPromoterMESVAvailable sinceJuly 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T
Plasmid#183364PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationH342D_K345M_K379TPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4b
Plasmid#183365PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4b (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMSCV-IRES-GFP-dog-Caspase-1/4a
Plasmid#183363PurposeStable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transductionDepositorInsertCaspase-1/4a (CASP4 Canis lupus familiaris)
UseRetroviralTagsMycExpressionMutationPromoterMESVAvailable sinceMay 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
BicC.Ca9.R
Plasmid#168295PurposeExpresses Cas9 in Drosophila late germline cellsDepositorInsertBicC.Cas9-T2A-eGFP-p10
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pStr-KDEL_APV-SBP-Dendra2-ER-5
Plasmid#170679PurposeExpresses ER-targeted streptavidin-KDEL (hook) and SBP-Dendra2 targeted to the ER lumen with an N-terminal APV tripeptide (cargo) for RUSH.DepositorInsertsAPV-SBP-Dendra2-ER-5
streptavidin-KDEL
UseTagsExpressionMammalianMutationPromoterIRES and T7Available sinceJan. 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
p-sfGFP-pA-FRT-Amp-FRT
Plasmid#176620Purposefor generating PCR templates with sfGFP and Amp for BAC recombinationDepositorInsertsuper folder green fluorescent protein with FRT-flanked ampicillin cassette
UseUnspecifiedTagsExpressionMutationH217R - please see dep. commentsPromoterAvailable sinceNov. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
TOMM20-mTurquoise-RhoG-QL-deltaCaaX
Plasmid#176122PurposeConstitutively active, mitochondiral localized RhoGDepositorInsertRHOG (RHOG Human)
UseTagsExpressionMammalianMutationQLPromoterAvailable sinceOct. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
Negative feedback (NF) plasmid
Plasmid#169747PurposeStandalone NF circuit plasmid that has the same implementation of the NF subcircuit in Equalizers.DepositorInserttetR-P2A-eGFP
UseSynthetic BiologyTagsExpressionMammalianMutationPromoterCMV-tetO2Available sinceJune 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pDOC-K-glmS-GFPrev
Plasmid#158060PurposeDonor plasmid for insertion of eGFP and the constitutive acpP promoter into the S. Typhimurium chromosome. GFP is in the reverse orientation relative to the kanamycin resistance selectable markerDepositorInsertGreen Fluorescent Protein optimised for excitation with UV light
UseSynthetic BiologyTagsExpressionBacterialMutationPromoteracpPAvailable sinceSept. 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
FH95pUP95S73AGW (F2)
Plasmid#74039Purposelentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusionDepositorInsertPsd95
UseLentiviral and RNAiTagsExpressionMammalianMutationS73APromoterH1Available sinceAug. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pJUMP19-Tac
Plasmid#126958PurposeTerminator acceptor vector. Minimal cargo vector; with eGFP in downstream secondary site and constitutive promoter J23100 in upstream site. Allows introducing any part using BsmBI.DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionBacterialMutationNonePromoterAvailable sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTrcHis-7D12Asp73TAG
Plasmid#125266PurposeExpresses 7D12 anti-EGFR nanobodies with UAG codon at Asp73 for synthetic amino acid in combination with pEVOL-pAzFDepositorInsert7D12 Asp73TAG
UseTagsHis-TagExpressionBacterialMutationPromoterAvailable sinceMay 31, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCF736
Plasmid#121893PurposeEF1a-dTEV-protease-GFPDepositorInsertsTEV protease
eGFP
UseLentiviralTagsExpressionMutationPromoterEF-1a core promoterAvailable sinceMarch 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pTHRB-donor
Plasmid#112333PurposeCRISPR donor plasmid to tag human transcription factor THRB with GFPDepositorInsertTHRB homology arms flanking EGFP-IRES-Neo cassette (THRB Human)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pATF1-donor
Plasmid#112328PurposeCRISPR donor plasmid to tag human transcription factor ATF1 with GFPDepositorInsertATF1 homology arms flanking EGFP-IRES-Neo cassette (ATF1 Human)
UseCRISPRTagsExpressionMutationhg38:12:50819876:T>A, hg38:12:50819888:T>C,…PromoterAvailable sinceNov. 21, 2018AvailabilityAcademic Institutions and Nonprofits only