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enOgeuIscB with ωRNA-v13

Plasmid

#220940

Purpose

CMV-driven expression of enOgeuIscB and human U6-driven expression of ωRNA-v13 scaffold with BsmBI for guide cloing

Depositor

Weixin Tang

Publication

Yan et al Nat Chem Biol. 2024 Jul 8. doi: 10.1038/

Inserts

CMV-SV40 NLS-enOgeuIscB_nucleoplasmin NLS
OgeuIscB-ωRNA-v13 scaffold with BsmBI for guide cloning

Use

CRISPR

Expression

Mammalian

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Syn-pAceR

Plasmid

#195521

Purpose

Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

Depositor

Vincent Pieribone

Publication

Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

Insert

pAceR

Use

AAV

Expression

Mammalian

Mutation

VARNAM 78E, 81D, 92N; SI linker

Promoter

Synapsin

Available since

Jan. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pScalps Luciferase Zsgreen

Plasmid

#184784

Purpose

Lentiviral vector with luciferase gene under SFFV promoter and Zsgreen under Cyclophilin promoter

Depositor

Ajit Chande

Publication

Mishra et al Front Cell Infect Microbiol. 2021 Apr 23

Insert

Firefly Luciferase and Zsgreen

Use

Lentiviral and Luciferase

Promoter

SFFV

Available since

Oct. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV-ND1 Right-GSVG-UGI

Plasmid

#187413

Purpose

human mitochondrial DNA ND1 target TALE

Depositor

Jin-Soo Kim

Publication

Mok et al Nat Commun. 2022 Jul 12;13(1):4038. doi:

Insert

DddA GSVG variant

Expression

Mammalian

Available since

Aug. 4, 2022

Availability

Academic Institutions and Nonprofits only

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AAV8-cPE-C

Plasmid

#183539

Purpose

Deliver a split compact Prime Editor in vivo

Depositor

Wen Xue

Publication

Zheng et al Mol Ther. 2022 Mar 2;30(3):1343-1351. do

Insert

C-terminal fragment of SpCas9 nickase (H840A), Reverse Transcriptase

Use

AAV

Expression

Mammalian

Promoter

U1A

Available since

June 24, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE-GCaMP6s-WPRE-sv40

Plasmid

#184414

Purpose

AAV expression of Fluorescent calcium reporter, GCaMP6s from TRE promoter

Depositor

Marylka Yoe Uusisaari

Publication

Dorgans et al Front Cell Neurosci. 2022 Apr 27;16:8250

Insert

GCaMP6s

Use

AAV

Expression

Mammalian

Promoter

TRE

Available since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pVITRO1-BFP-CAAX

Plasmid

#184459

Purpose

Plasma membrane-targeted EBFP2 expression in mammalian cells

Depositor

Chuan-Hsiang Huang

Publication

Yang et al Cell. 2021 Dec 9;184(25):6193-6206.e14.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

EBFP2-CAAX

Expression

Mammalian

Mutation

WT

Promoter

Rat EF1α

Available since

May 26, 2022

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pEGFP-N1 VPS50

Plasmid

#175500

Purpose

VPS50-GFP is expressed in mammalian cells

Depositor

Juan Bonifacino

Publication

Schindler et al Nat Cell Biol. 2015 May;17(5):639-50. do

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Vps50 (Vps50 Mouse)

Tags

EGFP

Expression

Mammalian

Available since

Nov. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pKD279.8

Plasmid

#173171

Purpose

Encodes expression of SO_4388(REC)-PsdR(DBD) 137 and an sfGFP reporter

Depositor

Jeffrey Tabor

Publication

Cartwright et al Proc Natl Acad Sci U S A. 2021 May 18;11

Inserts

SO_4388(REC)-PsdR(DBD) 137
sfGFP

Expression

Bacterial

Promoter

J23108 and PpsdA110

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D

Plasmid

#107864

Purpose

Bacterial expression of PIS and PI4K

Depositor

Sanford Simon

Publication

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD

Available since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pVEGFR1_TEV(C)_TCS(Q'G)_NLS-HA-dCas9(C)

Plasmid

#101105

Purpose

Encodes dCas9(C) (without VP64) fused to VEGFR1

Depositor

Tudor Fulga

Publication

Baeumler et al Cell Rep. 2017 Sep 12;20(11):2639-2653.

Insert

VEGFR1-dCas9(C)

Use

CRISPR and Synthetic Biology

Tags

HA

Expression

Mammalian

Available since

Nov. 13, 2017

Availability

Academic Institutions and Nonprofits only

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pVITRO1-SS-113

Plasmid

#68737

Purpose

ZF9-TF with mCherry marker

Depositor

James Collins

Publication

Slomovic et al Nat Methods. 2015 Sep 21. doi: 10.1038/n

Inserts

ZF9-VP64
mCherry

Tags

NLS and flag

Expression

Mammalian

Available since

Oct. 19, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pBT305_pBS-U-attPx3-FRT5-pSV40-Neo-pA-PL-FRT5

Plasmid

#52539

Purpose

Expression of FRT5-flanked Neo; homologous recombination targeting vector cloning

Depositor

Liqun Luo

Publication

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attPx3 (70 bp each)
Neo

Expression

Mammalian

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-1-p19-T7-PLK1

Plasmid

#46312

Depositor

Judy Lieberman

Publication

Huang et al Nat Biotechnol. 2013 Apr;31(4):350-6. do

Inserts

p19
PLK1 DNA-1
linker
PLK1 DNA-2

Use

Pro-sirna

Tags

6XHis and GST

Expression

Bacterial

Available since

Jan. 2, 2014

Availability

Academic Institutions and Nonprofits only
pCMV-Tag2B Flag BimEL(TA)

Plasmid

#23093

Depositor

Roger Davis

Publication

Hubner et al Mol Cell. 2008 May 23. 30(4):415-25.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Bim EL (TA) (Bcl2l11 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

Changed Threonine 112 to Alanine

Available since

Feb. 26, 2010

Availability

Academic Institutions and Nonprofits only
pMMK ENTR 2

Plasmid

#206261

Purpose

ENTR Vector 1 for MultiSite Gateway assembly and production of recombinant baculovirus using MultiMate assembly. Empty backbone.

Depositor

Imre Berger

Publication

Aulicino et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Type

Empty backbone

Use

Multimate/gateway entr 2

Available since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pENTR L3-eGFP-Myc-L2

Plasmid

#32314

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

eGFP-Myc

Use

Gateway multisite entry vector

Tags

Myc

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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LHD(B)-NovoSplit657(B)-mNeonGreen

Plasmid

#255884

Purpose

Expression plasmid for NovoSplit657(B), the second part of the NovoSplit system, fused to mNeonGreen for fluorescence labeling, and LHD(B)

Depositor

Julia Mahamid

Publication

Tran et al Science. 2026 Jul 16:eaeb0822. doi: 10.1

Insert

NovoSplit657(B)

Tags

LHD(B) and mNeonGreen

Expression

Mammalian

Promoter

CMV

Available since

July 20, 2026

Availability

Academic Institutions and Nonprofits only

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Iron Insensitive Control Construct 2

Plasmid

#243007

Purpose

Iron Insensitive Control Construct under the same expression promoter used in IronFist

Depositor

Roland Malli

Publication

Akyol et al ACS Sens. 2025 Aug 22;10(8):5854-5861. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Inserts

mNeonGreen
T2A
mCherry

Use

Gateway Cloning

Expression

Mammalian

Available since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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2xMyc-tdMCP-SNAP (tdMCP standard)

Plasmid

#226166

Purpose

For transient expression of the tdMCP-protein standard. Used in LABEL-seq abundance assays.

Depositor

Dustin Maly

Publication

Simon et al Nat Methods. 2024 Nov;21(11):2094-2106.

Insert

2xMyc-tdMCP-SNAPtag

Use

Lentiviral

Tags

2xMyc-tdMCP and EGFP-IRES-BlastR

Expression

Mammalian

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

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