We narrowed to 10,431 results for: plasmids 101

1. pSF4 TetCMV intron 20xGCN4 Renilla Rh1 STOP 24xMS2v5 SV40 CTE polyA

   Plasmid

   #105000

   Purpose

   Expresses ST-Renilla-Rh1 calibration reporter in mammalian cells; note plasmid contains 24xGCN4 repeats

   Depositor

   Jeffrey Chao

   Article

   Voigt et al Cell Rep. 2017 Dec 26;21(13):3740-3753.

   Insert

   Renilla luciferase
   

   Tags

   24xGCN4 repeats (SunTag), 24xMS2v5 stem loops in …

   Expression

   Bacterial and Mammalian

   Promoter

   TetCMV

   Available Since

   Feb. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAV-EF1a-DIO-FLPo-Myc

   Plasmid

   #124641

   Purpose

   AAV-mediated Cre-dependent expression of FLPo recombinase under EF1a promoter

   Depositor

   David Dupret

   Article

   Trouche et al Cell. 2019 Mar 7;176(6):1393-1406.e16. d

   Insert

   FLPo
   

   Use

   AAV and Cre/Lox

   Tags

   myc tag

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. EF1a_KLF4_P2A_Hygro_Barcode

   Plasmid

   #120455

   Purpose

   Barcoded lentiviral vector to express KLF4 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF4 (KLF4 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. anti-Notch1_E6-pBIOCAM5

   Plasmid

   #39344

   Depositor

   John McCafferty

   Article

   Falk et al Methods. 2012 Sep;58(1):69-78. doi: 10.1

   Insert

   scFv-Fc-fusion specific for Notch1 (clone B9) (Notch1, NOTCH1 Mouse, Human)
   

   Tags

   3xFLAG, 6xHis, and human Fc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. INS-GFP-CD19-EPT

   Plasmid

   #210469

   Purpose

   Donor plasmid for knock-in GFP-CD19 into the human INS locus

   Depositor

   Kai Wang

   Article

   Zhao et al STAR Protoc. 2024 May 23;5(2):103084. do

   Insert

   INS homologous recombination arms with a GFP-CD19 and EF1alpha-drived puromycin-NLS-tdTomato selection cassette (INS Human)
   

   Use

   CRISPR

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SPACE-ΔUGI (pRZ1836)

   Plasmid

   #140243

   Purpose

   CMV promoter expression plasmid for TadA*(V82G)-nCas9-pmCDA1(R187W)-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Grunewald et al Nat Biotechnol. 2020 Jun 1. pii: 10.1038

   Insert

   SPACE-ΔUGI
   

   Expression

   Mammalian

   Mutation

   V82G in TadA*, D10A in Cas9, R187W in pmCDA1

   Promoter

   CMV

   Available Since

   June 3, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. CGBE1-VRQR (pBM1192)

   Plasmid

   #140254

   Purpose

   CMV promoter expression plasmid for UNG(E.coli)-rAPOBEC1(R33A)-nCas9_VRQR-P2A-EGFP

   Depositor

   Keith Joung

   Article

   Kurt et al Nat Biotechnol. 2020 Jul 20. pii: 10.103

   Insert

   eUNG-BE4max(R33A)_VRQR-ΔUGI
   

   Expression

   Mammalian

   Mutation

   R33A in rAPOBEC1, VRQR mutations in SpCas9, D10A …

   Promoter

   CMV

   Available Since

   Aug. 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hSyn-DIO {mCAR}off-{ChETA}on-WPRE

   Plasmid

   #111387

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF mCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   mCAR (Cxadr Mouse)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEX-CFP-STIM1-deltaK

   Plasmid

   #18860

   Depositor

   Tobias Meyer

   Article

   Liou et al Proc Natl Acad Sci U S A. 2007 May 29. 1

   Insert

   STIM1 (STIM1 Human)
   

   Tags

   CFP

   Expression

   Mammalian

   Mutation

   Stop codon at amino acid position 671 of STIM1. C…

   Available Since

   Oct. 15, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPB-CAG-Klf2.2A.Nanog-Cherry-pA-pgk-zeo

    Plasmid

    #74919

    Purpose

    Mammalian expression of mKlf2 and mNanog-mCherry

    Depositor

    Jose Silva

    Article

    dos Santos et al Cell Stem Cell. 2014 Jul 3;15(1):102-10.

    Inserts

    Klf2 (Klf2 Mouse)
    Nanog (Nanog Mouse)
    

    Tags

    T2A and mCherry

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 26, 2016

    Availability

    Academic Institutions and Nonprofits only

11. Coexpressed Free GFP-NES

    Plasmid

    #182491

    Purpose

    Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and fusion protein GFP-AcNES1 (GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

    Depositor

    Claudia Vickers

    Article

    Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

    Inserts

    FPPS
    GFP-NES
    

    Use

    Synthetic Biology; Metabolic engineering

    Expression

    Yeast

    Promoter

    GAL10 and GAL7

    Available Since

    Nov. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAV2_hSyn_iChloC_CA_Citrine

    Plasmid

    #98220

    Purpose

    Slow cycling (open for minutes) step-function artificial anion conducting channelrhodopsin (aACR). High light sensitivity. Activation with blue to green light, inactivation max with 605 nm (accelarates closure to ms). Codon optimized for mammalian expression.

    Depositor

    Simon Wiegert

    Article

    Wietek et al Sci Rep. 2017 Nov 2;7(1):14957. doi: 10.

    Insert

    Synthetic construct iChloC_C128A gene
    

    Use

    AAV

    Tags

    Citrine

    Expression

    Mammalian

    Mutation

    E83Q, E90R, E101S, C128A, T159C, D156N

    Promoter

    human synapsin

    Available Since

    Oct. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pKLV2-U6gRNA5(Stat3)-PGKpuroBFP-W

    Plasmid

    #105027

    Purpose

    Lentiviral gRNA plasmid targeting mouse Stat3 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Stat3 (Stat3 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAWp28-v1 scaffold

    Plasmid

    #157976

    Purpose

    pBT264-U6p-{2xBbsI}-v1 sgRNA scaffold-{MfeI} Note: This plasmid is unstable. Screen multiple colonies to identify full length clones.

    Depositor

    Alan Siu Lun Wong

    Article

    Zhou et al Cell Rep. 2020 Aug 11;32(6):108020. doi:

    Type

    Empty backbone

    Available Since

    May 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPRIME-CMV-GFP-shCrh1

    Plasmid

    #132709

    Purpose

    Encodes short hairpin RNA (shRNA) #1 that targets the 3’-untranslated region of the rat Crh gene

    Depositor

    Robert Messing

    Article

    Pomrenze et al Cell Rep. 2019 Oct 1;29(1):13-21.e4. doi

    Insert

    shCrh(1) (Crh Rat)
    

    Use

    Lentiviral

    Promoter

    CMV

    Available Since

    Oct. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLL6 sgRNA(MS2)-MS2-hA3a D131E

    Plasmid

    #112132

    Purpose

    empty sgRNA cloning vector with MS2-humanA3a D131E

    Depositor

    Fei-Long Meng

    Article

    Liu et al Cell Rep. 2018 Oct 23;25(4):884-892.e3.

    Insert

    human Apobec3a D131E mutant (APOBEC3A Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    D131E mutant in hA3a

    Promoter

    hU6,CBh

    Available Since

    Sept. 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONOR-SNCAe3-WT

    Plasmid

    #85847

    Purpose

    Donor plasmid for SNCA exon3 wild type sequence. lso contains TagBFP and dTomato

    Depositor

    Jens Schwamborn

    Article

    Arias-Fuenzalida et al Stem Cell Reports. 2017 Nov 14;9(5):1423

    Insert

    SNCA exon 3 homology arms (SNCA Human)
    

    Expression

    Bacterial and Mammalian

    Available Since

    May 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pKLV2-U6gRNA5(hEGFR_2b)-PGKpuro2ABFP-W

    Plasmid

    #208432

    Purpose

    Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W

    Plasmid

    #208431

    Purpose

    Lentiviral gRNA plasmid targeting human EGFR gene, co-expression of BFP tag

    Depositor

    Sok Ching Cheong

    Article

    Chai et al Heliyon. 2024 Oct 22;10(21):e39677. doi:

    Insert

    EGFR (EGFR Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    N/A

    Promoter

    human U6

    Available Since

    July 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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20. pBiFC-SS-VN155(I152L)-IRE1-dLD

    Plasmid

    #217752

    Purpose

    Expresses the VN fragment of BiFC-IRE1-dLD

    Depositor

    Christina Chan

    Article

    Cho et al Cell Rep. 2019 Apr 2;27(1):307-320.e5. d

    Insert

    Serine/threonine-protein kinase/endoribonuclease IRE1 (ERN1 Human)
    

    Tags

    Venus (1-154, I152L)

    Expression

    Mammalian

    Mutation

    deleted amino acids 29-408

    Available Since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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