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pTC-ApoE-Tet

Plasmid

#85578

Purpose

Sleeping beauty transposon for hydrodynamic tail vein injection for CreER expression and tetracyclin-dependent transgene/shRNA expression (ApoE.HCR.hAAT promoter ) in mouse liver

Depositor

Ursula Ehmer

Article

Hubner et al J Gene Med. 2016 Dec 23. doi: 10.1002/jg

Insert

CreER

Use

Cre/Lox

Expression

Mammalian

Promoter

ApoE.HCR.hAAT

Available Since

Feb. 22, 2017

Availability

Academic Institutions and Nonprofits only

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pBbA5c-MevT-MBIS

Plasmid

#35150

Depositor

Jay Keasling, Taek Soon Lee

Article

Peralta-Yahya et al Nat Commun. 2011 Sep 27;2:483. doi: 10.1

Insert

AtoB(o)-HMGS(o)-HMGR(o)-MK(o)-PMK(o)-PMD-Idi-IspA

Use

Synthetic Biology

Expression

Bacterial

Promoter

LacUV5,

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only

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pMpGWB206

Plasmid

#68597

Purpose

Gateway binary vector designed for transgenic research with Marchantia polymorpha as well as other plants

Depositor

Takayuki Kohchi

Article

Ishizaki et al PLoS One. 2015 Sep 25;10(9):e0138876. do

Type

Empty backbone

Tags

Citrine

Expression

Plant

Promoter

Cauliflower mosaic virus 35S promoter

Available Since

June 24, 2016

Availability

Academic Institutions and Nonprofits only

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pRenilla

Plasmid

#118059

Purpose

GBPart - Renilla Luciferase CDS

Depositor

Koen Venken

Article

Sarrion-Perdigones et al Nat Commun. 2019 Dec 13;10(1):5710. doi:

Insert

Renilla

Use

Luciferase and Synthetic Biology; Gbpart

Available Since

Dec. 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti CMV/TO DEST (775-1)

Plasmid

#17432

Purpose

3rd gen lentiviral Gateway destination vector, expression, CMV/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Type

Empty backbone

Use

Lentiviral; Destination vector

Expression

Mammalian

Available Since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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PB-TET

Plasmid

#20909

Purpose

piggyBac empty vector with Gateway cassette, tet inducible promoter, and linked reporter (IRES-bgeo)

Depositor

Andras Nagy

Article

Woltjen et al Nature. 2009 Apr 9;458(7239):766-70. doi

Insert

Gateway Destination Vector

Use

piggybac

Tags

IRES-bGeo

Expression

Mammalian

Available Since

May 5, 2009

Availability

Academic Institutions and Nonprofits only

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MAC-tag-C

Plasmid

#108077

Purpose

Gateway compatible destination vectors containing StrepIII/HA/BioID

Depositor

Markku Varjosalo

Article

Liu et al Nat Commun. 2018 Mar 22;9(1):1188. doi:

Insert

CCDB (ccdB(letD) )

Tags

StrepIII-HA-BirA*

Expression

Bacterial and Mammalian

Available Since

April 26, 2018

Availability

Academic Institutions and Nonprofits only

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pC018 - LshCas13a from Leptotrichia shahii

Plasmid

#91908

Purpose

Expresses LshCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LshCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pSF280

Plasmid

#167675

Purpose

Intermediate vector for six gRNAs using GoldenGate, contains ccdB and attL1-L2 sites for Gateway

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

attL1-ccdB-attL2

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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MmXYRS-4xM15

Plasmid

#155343

Purpose

Plasmid encoding the translational pair that enables efficient incorporation of multiple phenylalanine-based amino acids in response to the amber codon into proteins in mammmalian cells.

Depositor

Irene Coin

Article

Bottke et al EMBO Rep. 2020 Sep 14:e50437. doi: 10.15

Insert

Humanized MmXYRS/tRNAM15

Expression

Mammalian

Promoter

CMV/U6

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pMSP1E3D1_D73C

Plasmid

#39328

Depositor

Stephen Sligar

Article

Ritchie et al Methods Enzymol. 2009;464:211-31. doi: 1

Insert

MSP1E3D1_D73C (APOA1 synthetic gene)

Tags

6xHis

Expression

Bacterial

Mutation

cys mutant of MSP1E3D1

Promoter

T7

Available Since

Sept. 11, 2012

Availability

Academic Institutions and Nonprofits only
pRU324

Plasmid

#167691

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pC020 - LseCas13a from Listeria seeligeri

Plasmid

#91910

Purpose

Expresses LseCas13a for bacterial expression and contains backbone for spacer cloning

Depositor

Feng Zhang

Article

Abudayyeh et al Nature. 2017 Oct 4. doi: 10.1038/nature2

Insert

LseCas13a and guide expression cassette

Expression

Bacterial

Available Since

Oct. 4, 2017

Availability

Academic Institutions and Nonprofits only

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pDest_pK7WG2_pRPS5A_CTP OleGFP

Plasmid

#171723

Purpose

ptpTALECD vector assembly, Destination vector;

Depositor

Shin-ichi Arimura

Article

Nakazato et al Nat Plants. 2021 Jul;7(7):906-913. doi:

Insert

RPS5A promoter, RecA1 plastid targeting signal upstream of attR1, GFP downstream of attR2

Expression

Plant

Available Since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pUBQ10-SNAP-GW

Plasmid

#209350

Purpose

Gateway cloning compatible binary vector for agrobacterium mediated transformation. Arabidopsis UBQ10 promoter expression of N-terminal SNAP-tag fusion protein.

Depositor

Lucia Strader

Article

Allen et al BMC Res Notes. 2025 Apr 8;18(1):149. doi

Type

Empty backbone

Expression

Plant

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCW57.1-3xFLAG-NLS-GFP

Plasmid

#198238

Purpose

Lentivector with tet-inducible 3xFLAG-NLS-GFP

Depositor

Stephen Tapscott

Article

Nip et al Hum Mol Genet. 2023 Feb 2:ddad021. doi:

Insert

3xFLAG-NLS-GFP

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Promoter

TRE

Available Since

May 11, 2023

Availability

Academic Institutions and Nonprofits only

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pET-28a(+)::NL

Plasmid

#141289

Purpose

NLUC in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)

Depositor

Andrew Millar

Article

Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10

Insert

NLUC

Use

Luciferase

Expression

Bacterial

Mutation

Engineered for high stability (t1/2 = 11.5 days a…

Promoter

promoter for bacteriophage T7 RNA polymerase

Available Since

Feb. 17, 2021

Availability

Academic Institutions and Nonprofits only

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AEB-Theo45

Plasmid

#63850

Purpose

This pFTV1 vector contains a theophylline binding riboswitch, controlling the translation initaition rate of mRFP1 gene.

Depositor

Howard Salis

Article

Espah Borujeni et al Nucleic Acids Res. 2015 Nov 30. pii: gkv

Insert

Theo45 theophylline riboswitch

Use

Synthetic Biology

Promoter

J23100

Available Since

Feb. 19, 2016

Availability

Academic Institutions and Nonprofits only

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Sth1Cas9n cytosine base editor

Plasmid

#194209

Purpose

Sth1Cas9-D9A-nickase_rAPOBEC1_cytosine base editor for RNA recording

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

Sth1Cas9-D9A nickase

Use

CRISPR

Expression

Bacterial

Mutation

Changed Aspartate 9 to Alanine.

Available Since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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pCBC-MT3T4

Plasmid

#50595

Purpose

CRISPR/Cas based plant genome editing and gene regulation; used as template for making expression cassette with multiple gRNA target sites

Depositor

Qi-Jun Chen

Article

Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.

Insert

T3, gRNA scaffold, U6-26t plus, OsU3p, T4

Use

CRISPR; Pcr template

Expression

Plant

Available Since

Dec. 11, 2014

Availability

Academic Institutions and Nonprofits only

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