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pETduet+p300HAT

Plasmid

#157793

Purpose

Expression of p300 HAT domain

Depositor

Michael Rosen

Article

Gibson et al Cell. 2019 Oct 3;179(2):470-484.e21. doi

Insert

p300HAT (EP300 Human)

Tags

His6

Expression

Bacterial

Promoter

T7

Available Since

Aug. 5, 2020

Availability

Academic Institutions and Nonprofits only

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2Flag-pDEST-N

Plasmid

#118371

Purpose

Gateway compatible mammalian expression vector with N-terminal 2Flag to tag proteins of interest.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

2Flag

Expression

Mammalian

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pCdrA-gfpC

Plasmid

#111614

Purpose

Fluorescence-based gauging of the level of the nucleotide secondary messenger cyclic di-GMP in Pseudomonas aeruginosa and related species

Depositor

Tim Tolker-Nielsen

Article

Rybtke et al Appl Environ Microbiol. 2012 Aug;78(15):

Insert

PcdrA-RBSII-gfp(Mut3)-T0-T1

Expression

Bacterial

Available Since

June 4, 2018

Availability

Academic Institutions and Nonprofits only

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tTA-IRES-Neo

Plasmid

#16541

Depositor

Bert Vogelstein

Article

Yu et al Proc Natl Acad Sci U S A. 1999 Dec 7. 96

Insert

tTA

Expression

Mammalian

Available Since

April 16, 2015

Availability

Academic Institutions and Nonprofits only
Tol2-zTRAP

Plasmid

#42236

Depositor

Stephen L Johnson

Article

Tryon et al Genesis. 2012 Dec 19. doi: 10.1002/dvg.2

Insert

EGFP-Rpl10a (rpl10a Zebrafish)

Tags

EGFP

Available Since

March 7, 2013

Availability

Academic Institutions and Nonprofits only

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pACYC-T7

Plasmid

#41187

Depositor

Dan Bolon

Article

Wayne et al J Biol Chem. 2007 Nov 30;282(48):35386-9

Insert

S. cerevisiae Hsp82

Tags

6xHis

Expression

Bacterial

Promoter

T7

Available Since

Nov. 30, 2012

Availability

Academic Institutions and Nonprofits only

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pCMV-tBID

Plasmid

#21149

Depositor

Junying Yuan

Article

Li et al Cell. 1998 Aug 21. 94(4):491-501.

Insert

pCMV-tBID

Tags

flag

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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pFA6a-6xGLY-V5-kanMX6

Plasmid

#20780

Depositor

Mark Hochstrasser

Article

Funakoshi et al Yeast. 2009 Feb 25. 26(3):185-192.

Type

Empty backbone

Use

Pcr-based c-terminal tagging

Expression

Yeast

Available Since

March 23, 2009

Availability

Academic Institutions and Nonprofits only

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pFH2.10_Gag-MCP-P2A-eGFP

Plasmid

#205525

Purpose

Express HIV Gag-MCP-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pDL278_P23-DsRed-Express2

Plasmid

#121505

Purpose

E. coli-streptococcal shuttle vector that expresses rfp

Depositor

Robert Shields

Article

Shields et al Appl Environ Microbiol. 2019 May 17. pii

Insert

DsRed-Express2

Use

E. coli streptococcal shuttle vector

Promoter

P23

Available Since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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CMV-ShadowY

Plasmid

#104621

Purpose

Expresses ShadowY in mammalian cells

Depositor

Hideji Murakoshi

Article

Murakoshi et al Sci Rep. 2017 Jul 28;7(1):6791. doi: 10.

Insert

ShadowY

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 5, 2018

Availability

Industry, Academic Institutions, and Nonprofits

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pGEx-4T-1-YTHDF2

Plasmid

#52301

Purpose

Encode YTHDF2 isoform 2 with N-terminal GST tag for E. coli expression

Depositor

Chuan He

Article

Wang et al Nature. 2014 Jan 2;505(7481):117-20. doi

Insert

YTHDF2 (YTHDF2 Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

April 14, 2014

Availability

Academic Institutions and Nonprofits only
pBT264

Plasmid

#27438

Depositor

Liqun Luo

Article

Miyamichi et al Nature. 2011 Apr 14;472(7342):191-6. doi

Insert

TRE-tdTomato-myc flanked by insulators

Use

Mouse Targeting; Tta dependent

Tags

Myc

Available Since

March 18, 2011

Availability

Academic Institutions and Nonprofits only

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Gal4-TEAD4

Plasmid

#24640

Depositor

Kunliang Guan

Insert

TEAD-4 (TEAD4 Human)

Tags

Gal4

Expression

Mammalian

Available Since

May 13, 2010

Availability

Academic Institutions and Nonprofits only
pGART-GFP

Plasmid

#99106

Purpose

Expresses human GART-GFP fusion in mammalian cells

Depositor

Stephen Benkovic

Article

An et al Science. 2008 Apr 4;320(5872):103-6. doi

Insert

GART (GART Human)

Tags

GFP

Expression

Mammalian

Mutation

A65T, L68V, and A72S mutations in GFP gene; kanR/…

Promoter

CMV

Available Since

Dec. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_iSH2-pMagFast2(3x)-iRFP

Plasmid

#67298

Purpose

Blue light dependent photoswitching system

Depositor

Moritoshi Sato

Article

Kawano et al Nat Commun. 2015 Feb 24;6:6256. doi: 10.

Insert

iSH2-pMagFast2(3x)-iRFP (PIK3R1 Synthetic, Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 9, 2015

Availability

Academic Institutions and Nonprofits only

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pCDNA3 Flag MKK7B2Jnk3a2

Plasmid

#19729

Depositor

Roger Davis

Article

Lei et al Mol Cell Biol. 2002 Jul . 22(13):4929-42

Insert

MKK7B2Jnk3a2 (Map2k7, MAPK10 Mouse, Human)

Tags

Flag

Expression

Mammalian

Available Since

March 31, 2009

Availability

Academic Institutions and Nonprofits only
pBabe puro H-Ras G12V E37G

Plasmid

#12275

Depositor

Bob Weinberg

Article

Rangarajan et al Cancer Cell. 2004 Aug . 6(2):171-83.

Insert

H-Ras G12V E37G (HRAS Human)

Use

Retroviral

Expression

Mammalian

Mutation

Contains G12V activating mutation. The E37G mutat…

Available Since

July 20, 2006

Availability

Academic Institutions and Nonprofits only
pCI neo-hEST2-HA

Plasmid

#1782

Depositor

Bob Weinberg

Article

Meyerson et al Cell 1997 Aug 22;90(4):785-95.

Insert

hTERT (TERT Human)

Tags

HA

Expression

Mammalian

Mutation

Clone is catalytically active but is dead in vivo.

Available Since

July 10, 2005

Availability

Academic Institutions and Nonprofits only
pHCMV_COCVmut_G

Plasmid

#207262

Purpose

Mammalian expression of a mutant Cocal virus G protein

Depositor

Feng Zhang

Article

Strebinger et al Nat Commun. 2023 Aug 23;14(1):5141. doi:

Insert

COCVmut_G

Expression

Mammalian

Mutation

K47A/Y209A/R354A; mammalian codon optimization

Promoter

CMV promoter

Available Since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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