165,307 results

1. PNCA-GFP

   Plasmid

   #67789

   Purpose

   Use together with pCMV-intron and VSV-G to package MMLV reporter virus

   Depositor

   Stephen Goff

   Article

   Ooi et al Epigenetics Chromatin. 2010 Sep 24;3(1):

   Insert

   MMLV-LTR-GFP
   

   Available Since

   Sept. 1, 2015

   Availability

   Academic Institutions and Nonprofits only

2. pDONR221_SLC6A8

   Plasmid

   #131915

   Purpose

   Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

   Depositor

   RESOLUTE Consortium, Giulio Superti-Furga

   Article

   Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

   Insert

   SLC6A8 (SLC6A8 Human)
   

   Expression

   Mammalian

   Available Since

   Oct. 15, 2019

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

3. mRFP-Rab5

   Plasmid

   #14437

   Purpose

   Mammalian expression of Rab5 fused to mRFP. Used for localization to the early endosome, and other early endosomal vacuoles.

   Depositor

   Ari Helenius

   Article

   Vonderheit et al PLoS Biol. 2005 Jul . 3(7):e233.

   Insert

   Rab 5 (RAB5A Human)
   

   Tags

   mRFP

   Expression

   Mammalian

   Available Since

   March 23, 2007

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. Kif5A-GFP-CIBN

   Plasmid

   #102252

   Purpose

   Expresses fusion of kinesin heavy chain 5A (1-572) with GFP and CIB1 (1-170)

   Depositor

   Bianxiao Cui

   Article

   Duan et al Chem Biol. 2015 May 21;22(5):671-82. doi

   Insert

   Kif5A-GFP-CIB1 (Kif5a, CIB1 Mustard Weed, Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pcDNA3.1 puro Nodamura B2

   Plasmid

   #17228

   Depositor

   Christopher Sullivan

   Article

   Sullivan et al J Virol. 2005 Jun . 79(12):7371-9.

   Insert

   Nodamura B2 (NoVsRNA1gp2 Nodamura Virus)
   

   Expression

   Mammalian

   Mutation

   2 silent point mutations

   Available Since

   June 22, 2011

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. pOT_6 - lenti-EFS-FMC6.3-BBz-2A-puro-2A-tNGFR

   Plasmid

   #181975

   Purpose

   Expresses anti-CD19 CAR (with 4-1BB signaling domain) linked to puromycin resistance via P2A and to human truncated NGFR via T2A for lentiviral delivery.

   Depositor

   Neville Sanjana

   Article

   Legut et al Nature. 2022 Mar;603(7902):728-735. doi:

   Insert

   FMC6.3-BBz CAR; tNGFR (NGFR Synthetic, Human)
   

   Use

   Lentiviral

   Mutation

   NGFR truncation: aa 11-276

   Available Since

   April 25, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. mWasabi-C1

   Plasmid

   #54743

   Purpose

   Localization: C1 Cloning Vector, Excitation: 493, Emission: 509

   Depositor

   Michael Davidson, Robert Campbell

   Article

   Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

   Type

   Empty backbone

   Tags

   mWasabi

   Expression

   Mammalian

   Available Since

   June 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pGL3-U6-sgRNA-EGFP

   Plasmid

   #107721

   Purpose

   For expression of sgRNA from the U6 promoter with EGFP expression simultaneously

   Depositor

   Xingxu Huang

   Article

   Liu et al Nat Commun. 2018 Jun 14;9(1):2338. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mCherry-hLC3B-pcDNA3.1

   Plasmid

   #40827

   Depositor

   David Rubinsztein

   Article

   Jahreiss et al Traffic. 2008 Apr;9(4):574-87. Epub 2008

   Insert

   LC3B (MAP1LC3B Human)
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 8, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pAAV_BiPVe4_ChR2_mCherry

    Plasmid

    #213937

    Purpose

    AAV construct expressing mCherry-tagged channelrhodopsin-2 driven by chandelier cell-targeting enhancer. Alias: pAAV_WDC0004_ChR2_mCherry

    Depositor

    Gordon Fishell

    Article

    Furlanis et al Neuron. 2025 May 21;113(10):1525-1547.e1

    Has Service

    AAV PHP.eB and AAV1

    Insert

    Channelrhodopsin 2-mCherry
    

    Use

    AAV

    Available Since

    July 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pOpen-T7gene1

    Plasmid

    #165505

    Purpose

    Highly processive DNA-dependent RNA polymerase derived from T7 bacteriophage

    Depositor

    Jennifer Molloy, Drew Endy, FreeGenes Project

    Insert

    T7 RNA Polymerase
    

    Use

    Synthetic Biology

    Available Since

    July 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

12. pET-MBP-Geo_st

    Plasmid

    #87700

    Purpose

    Expression plasmid for Cas9 from Geobacillus stearothermophilus with an N-Term MBP

    Depositor

    Jennifer Doudna

    Insert

    GeoCas9
    

    Tags

    10xHis-MBP-TEV

    Expression

    Bacterial

    Available Since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pU6-Sp-pegRNA-HEK3_CTT_ins

    Plasmid

    #132778

    Purpose

    S. pyogenes pegRNA for CTT insertion at the HEK3 site of human cells using prime editing

    Depositor

    David Liu

    Article

    Anzalone et al Nature. 2019 Oct 21. pii: 10.1038/s41586

    Insert

    HEK3_CTT_ins pegRNA
    

    Expression

    Mammalian

    Mutation

    See manuscript

    Promoter

    U6

    Available Since

    Oct. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. Nuclear Myosin I YFP

    Plasmid

    #60241

    Purpose

    Expresses nuclear isoform of myosin IC attached to YFP and an NLS

    Depositor

    Primal de Lanerolle

    Article

    Chuang et al Curr Biol. 2006 Apr 18;16(8):825-31.

    Insert

    Nuclear Myosin 1 (Myo1c Mouse)
    

    Tags

    EYFP and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. PB_TRE_sg30-PDGFR_EF1a_mCherry

    Plasmid

    #186309

    Purpose

    Plasmid for inducible mammalian cell expression of the sg30 helixCAM and constitutive expression of an identifying fluorescent protein (mCherry). Flanked with PiggyBac sites for easy line construction

    Depositor

    George Church

    Article

    Chao et al Cell. 2022 Aug 23. pii: S0092-8674(22)01

    Inserts

    sg30-PDGFR
    mCherry
    

    Tags

    His, myc

    Expression

    Mammalian

    Available Since

    Aug. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pICH-nuc-roGFP2-ORP1

    Plasmid

    #191758

    Purpose

    Ti plasmid pICH86988 harbouring nuc-roGFP2-ORP1 for peroxide detection in the nuclei of plant cells.

    Depositor

    Philip Mullineaux

    Article

    Exposito-Rodriguez et al bioRxiv 2024.01.18.576236

    Insert

    nuc-roGFP2-ORP1
    

    Use

    Binary agrobacterium ti plasmid

    Expression

    Plant

    Promoter

    CaMV 35S

    Available Since

    March 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLenti-EGFP-P4M-SidMx2

    Plasmid

    #136997

    Purpose

    Lentiviral EGFP lipid sensor for PI(4)P

    Depositor

    Ken-Ichi Takemaru

    Insert

    EGFP-P4M-SidMx2
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    EF1 alpha

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GFP-KASH2ext

    Plasmid

    #187018

    Purpose

    Control for inducible disruption of LINC Complex

    Depositor

    Jan Lammerding

    Article

    Earle et al Nat Mater. 2019 Dec 16. pii: 10.1038/s41

    Insert

    KASH2ext
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Aug. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. GPR87-DuET

    Plasmid

    #213293

    Purpose

    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

    Depositor

    Thomas Sakmar

    Article

    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

    Insert

    GPR87 (GPR87 Human)
    

    Tags

    1D4 and FLAG

    Expression

    Mammalian

    Promoter

    CMV, T7

    Available Since

    April 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. RAD21 C-tag CRISPR

    Plasmid

    #140540

    Purpose

    RAD21 tagging CRISPR

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    RAD21 (RAD21 Human)
    

    Use

    CRISPR

    Available Since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge