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pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

Plasmid

#62458

Purpose

expresses his-tagged C151S human Keap1 protein

Depositor

Yimon Aye

Article

Parvez et al J Am Chem Soc. 2014 Dec 31.

Insert

Keap1 (KEAP1 Human)

Tags

His-Halo-Tev

Expression

Mammalian

Mutation

Changed Cysteine 151 of Keap1 to serine

Promoter

CMV

Available Since

March 21, 2017

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-INPP4B WT

Plasmid

#161995

Purpose

Mammalian expression vector containing INPP4B. Also contains mCherry tag

Depositor

David Bryant

Article

Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

Insert

INPP4B (INPP4B Human)

Tags

mCherry

Expression

Mammalian

Available Since

Dec. 3, 2020

Availability

Academic Institutions and Nonprofits only

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pCW-OVOL2-HA

Plasmid

#184737

Purpose

Doxycycline-inducible lentiviral expression of OVOL2

Depositor

Alessia Ciarrocchi, Gloria Manzotti

Article

Gugnoni et al J Exp Clin Cancer Res. 2022 Mar 25;41(1)

Insert

ovo like zinc finger 2 (OVOL2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

Tet ON

Available Since

June 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Rheb(R15C)

Plasmid

#224290

Purpose

Cell transfection and expression of Rheb (R15C)

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

Rheb (RHEB Human)

Expression

Mammalian

Mutation

R15C

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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GEF-H1 FLARE212.pa

Plasmid

#134930

Purpose

GEF-H1 biosensor that does not have GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-212)-yPet-4X linker-Cerulean3-GEF-H1(213-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Nov. 2, 2020

Availability

Academic Institutions and Nonprofits only

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AAVS1-pur-CAG-mCherry

Plasmid

#159458

Purpose

AAVS1 targeting donor plasmid with mCherry expression cassette and puromycin selection gene

Depositor

Yuejun Chen

Article

Xiong et al Cell Stem Cell. 2020 Sep 16. pii: S1934-

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 8, 2020

Availability

Academic Institutions and Nonprofits only

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SiC-V2-Cas9G7

Plasmid

#133043

Purpose

Doxycycline-inducible SiC-V2 vector with Cas9G7 (sgRNA 7 targeting SpCas9 gene). In cells coexpressing Cas9 nuclease, this construct causes self-inactivation/editing of SpCas9 gene upon Dox addition.

Depositor

Sriram Neelamegham

Article

Kelkar et al Mol Ther. 2019 Sep 12. pii: S1525-0016(1

Insert

Tet repressor

Use

Lentiviral

Tags

Cerulean

Available Since

Nov. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pRRLSIN.cPPT.PGK-GFP-PTS1.WPRE

Plasmid

#133282

Purpose

Lentiviral expression of a peroxisomal eGFP

Depositor

Etienne Sokal

Article

Demaret et al Histochem Cell Biol. 2020 Mar 2. pii: 10

Insert

Peroxisome targeting signal 1

Use

Lentiviral

Tags

Peroxisome targeting signal 1

Expression

Mammalian

Promoter

None

Available Since

March 4, 2020

Availability

Academic Institutions and Nonprofits only

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pUC57-Amp_CRISPIE donor B5

Plasmid

#172846

Purpose

CRISPIE donor B5 (Zhong et al, eLife 2021), CDS of VCL exon21-mEGFP, translational phase (0-stop), excised by ACTB i4 sgRNA or DRS-1 sgRNA (with SpCas9).

Depositor

Haining Zhong

Article

Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

Insert

CRISPIE designer exon (phase 0-stop) encoding the CDS of VCL exon 21 fused to mEGFP

Use

CRISPR

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pCI-neo:alpha4-eGFP

Plasmid

#45068

Depositor

Henry Lester

Article

Srinivasan et al J Gen Physiol. 2011 Jan;137(1):59-79.

Insert

alpha4-eGFP (Chrna4 Mouse)

Tags

Green Fluorescent Protien and HA Tag

Expression

Mammalian

Promoter

CMV

Available Since

June 19, 2013

Availability

Academic Institutions and Nonprofits only

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pCMV-ABE7.10-P2A-GFP

Plasmid

#162509

Purpose

Express ABE7.10-P2A-GFP by CMV promoter in mammalian cells

Depositor

Yongsub Kim

Article

Shin et al Nucleic Acids Res. 2021 Feb 26;49(4):239

Insert

ABE7.10-P2A-GFP

Use

CRISPR

Tags

NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 9, 2020

Availability

Academic Institutions and Nonprofits only

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EF1a-mRuby2(miRE.FF4)-TS.FF4x2-bGH

Plasmid

#235294

Purpose

ComMAND closed-loop circuit regulating mRuby2 with 2 copies of microRNA target sites

Depositor

Kate Galloway

Article

Love et al Cell Syst. 2025 Apr 22:101269. doi: 10.1

Insert

mRuby2

Use

Synthetic Biology

Expression

Mammalian

Promoter

EF1a (human EF1a)

Available Since

May 14, 2025

Availability

Academic Institutions and Nonprofits only

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MSCV-Myc-IRES-GFP

Plasmid

#35396

Depositor

Martine Roussel

Article

Kawauchi et al Cancer Cell. 2012 Feb 14;21(2):168-80.

Inserts

Myc (Myc Mouse)
GFP

Use

Retroviral

Expression

Mammalian

Available Since

March 16, 2012

Availability

Academic Institutions and Nonprofits only
Neurl1-Flag pcDNA3.1

Plasmid

#17282

Depositor

Joan Conaway, Ronald Conaway

Article

Koutelou et al J Biol Chem. 2008 Feb 15;283(7):3846-53.

Insert

Neuralized-like (Neurl1a Mouse)

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 20, 2008

Availability

Academic Institutions and Nonprofits only
pDEST14-ARL3

Plasmid

#67376

Purpose

Expression of native hArl3 in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Bacterial

Promoter

T7

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
GEF-H1 FLARE191.pa

Plasmid

#134931

Purpose

GEF-H1 biosensor that has GEF activity.

Depositor

Klaus Hahn

Article

Azoitei et al J Cell Biol. 2019 Sep 2;218(9):3077-3097

Insert

GEF-H1(1-191)-yPet-4X linker-Cerulean3-GEF-H1(192-end)

Tags

None.

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Dec. 15, 2020

Availability

Academic Institutions and Nonprofits only

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pMXs-cMyc

Plasmid

#233183

Purpose

Retroviral expression of c-Myc

Depositor

Kate Galloway

Article

Wang et al Cell Syst. 2025 Apr 16;16(4):101205. doi

Insert

c-Myc (Myc Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

March 13, 2025

Availability

Academic Institutions and Nonprofits only

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p415 TEF roGFP2‐Tsa2ΔCPΔCR

Plasmid

#83239

Purpose

The catalytically inactive genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCPΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

Depositor

Tobias Dick

Article

Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

Insert

roGFP2-Tsa2dCpdCr

Tags

roGFP2 fusion with Tsa2dCpdCr

Expression

Yeast

Promoter

TEF

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pPEE47

Plasmid

#139426

Purpose

Recyclable marker for use in Cryptococcus neoformans. Contains the amdS2 counterselectable marker flanked by the mNeonGreen ORF and a flexible linker at the 5' end.

Depositor

James Fraser

Article

Spencer et al Fungal Genet Biol. 2020 May;138:103365.

Insert

mNeonGreen

Expression

Yeast

Promoter

TEF1

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-SEMPER_ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Plasmid

#221075

Purpose

SEMPER plasmid for expression of msfGFP[r5M] and mEBFP2 in ratios dictated by the trinucleotide TIS. Relative ORF expression is characterized in publication

Depositor

Mikhail Shapiro

Article

Duan et al Cell Syst. 2024 Jul 17;15(7):597-609.e4.

Insert

ACC-msfGFP[r5M]_ACC-mEBFP2_IRES-mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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