Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

More Filters

173,520 results

Showing: 7661 - 7680 of 173520 results

  7. EF1a-L274GMmMetRS-T2A-mCherry

    Plasmid
    #220800
    Purpose
    Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance
    Depositor
    Elizabeth Kirby
    Article
    Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18
    Insert
    Mars1 (Mars1 Mouse)
    Use
    Lentiviral
    Tags
    2xFLAG and T2A-mCherry
    Mutation
    mutation in MetRS to change aa 274 from L to G
    Promoter
    EF1a
    Available Since
    June 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    220800-plasmid-map-sequence-id-437285

    Enlarge

  8. ADRA2B-Tango

    Plasmid
    #66217
    Purpose
    Expression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocation
    Depositor
    Bryan Roth
    Article
    Kroeze et al Nat Struct Mol Biol. 2015 May;22(5):362-
    Insert
    ADRA2B (ADRA2B Human)
    Tags
    FLAG
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 15, 2015
    Availability
    Academic Institutions and Nonprofits only

  12. TDP-REGv2(mScarlet-FLAG)#9 in pTwist-CMV backbone

    Plasmid
    #216153
    Purpose
    Expresses mScarlet in cells with TDP-43 loss-of-function. Has one of the best dynamic ranges of those tested (~100-fold) in SK-N-BE2 cells (brighter than #7). It uses a single intron. [Code 'B11']. Note: It is not recommended to produce lentiviruses containing TDP-REG sequences – see Depositor Comments
    Depositor
    Pietro Fratta
    Article
    Wilkins et al Science. 2024 Oct 4;386(6717):61-69. doi
    Insert
    mScarlet with cryptic splice site and C-terminal FLAG tag
    Expression
    Mammalian
    Available Since
    July 1, 2024
    Availability
    Academic Institutions and Nonprofits only
    216153-plasmid-map-sequence-id-437757

    Enlarge

  15. pCDZ2FE_Champ1_1-812_2PA

    Plasmid
    #105676
    Purpose
    Expresses Flag-tagged full-length human Champ1 protein with mutations abolishing Rev7 binding
    Depositor
    Thanos Halazonetis
    Insert
    Champ1 (CHAMP1 Human)
    Tags
    2X Flag tag
    Expression
    Mammalian
    Mutation
    Mutations: P336A, P341A
    Available Since
    March 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    105676-plasmid-map-sequence-id-203181

    Enlarge

  19. TFORF1513

    Plasmid
    #143513
    Purpose
    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.
    Depositor
    Feng Zhang
    Article
    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi
    Insert
    NKX3-1 (NKX3-1 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    EF1a
    Available Since
    July 11, 2023
    Availability
    Academic Institutions and Nonprofits only
    143513-plasmid-map-sequence-id-400430

    Enlarge

Showing: 7661 - 7680 of 173520 results