165,307 results

1. pLX304_CMV::mCherry-LgBiT-FLAG-NES

   Plasmid

   #199715

   Purpose

   Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

   Depositor

   Alice Ting

   Article

   Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

   Insert

   mCherry-LgBiT-FLAG-NES
   

   Use

   Lentiviral and Luciferase

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3 HA E2F3B

   Plasmid

   #236438

   Purpose

   transient overexpression of E2F3B in mammalian cells

   Depositor

   Michele Pagano

   Article

   Clijsters et al Mol Cell. 2019 Jun 20;74(6):1264-1277.e7

   Insert

   E2F3B (E2F3 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   May 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR-hSyn-EGFP

   Plasmid

   #114215

   Purpose

   Lentiviral vector (control) that targets expression of EGFP to neurons

   Depositor

   Xue Han

   Article

   Keaveney et al Cell Rep. 2018 Jul 10;24(2):294-303. doi

   Insert

   Enhanced Green Fluorescent Protein
   

   Use

   Lentiviral and Mouse Targeting

   Tags

   None

   Expression

   Mammalian

   Mutation

   None

   Promoter

   human synapsin (hSyn)

   Available Since

   Sept. 25, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. Cx36-GCaMP

   Plasmid

   #123604

   Purpose

   Mouse Connexin 36-GCaMP3 fusion for measuring gap junction localized calcium signaling - expresses in mammalian cells

   Depositor

   John O'Brien

   Article

   Moore et al eNeuro. 2020 Mar 11. pii: ENEURO.0445-19

   Insert

   Connexin 36-GCaMP3 fusion (Gjd2 Mouse)
   

   Tags

   GCaMP3

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 31, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. gRNA_AAVS1-T2

   Plasmid

   #41818

   Purpose

   Expresses a guide RNA (gRNA) to target human AAVS1 (T2 target sequence) for genome engineering

   Depositor

   George Church

   Article

   Mali et al Science. 2013 Feb 15;339(6121):823-6. do

   Insert

   gRNA_AAVS1-T2
   

   Use

   CRISPR

   Expression

   Mammalian

   Available Since

   Jan. 11, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCDH-3xFLAG-TERT

   Plasmid

   #51631

   Purpose

   Mammalian expression of 3xFLAG tagged TERT, N-terminal.

   Depositor

   Steven Artandi

   Article

   Zhong et al Cell. 2012 Aug 3;150(3):481-94. doi: 10.

   Insert

   TERT (TERT Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Available Since

   May 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. PGK-AAVS1ZFNR

   Plasmid

   #60915

   Purpose

   encodes zinc finger nuclease specific to AAVS1 locus (right)

   Depositor

   Paul Gadue

   Article

   Sim et al Methods Mol Biol. 2015 Jan 29.

   Insert

   AAVS1-ZFNR
   

   Promoter

   PGK

   Available Since

   Feb. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCap-101-Hgy

   Plasmid

   #227508

   Purpose

   Capture plasmid with hygromycin resistance and pIJ101 replicon

   Depositor

   Chengzhang Fu

   Article

   Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

   Type

   Empty backbone

   Available Since

   Dec. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-TO-dCas9-GFP

   Plasmid

   #64106

   Purpose

   Expression of Sp dCas9-GFP in mammalian cells

   Depositor

   Thoru Pederson

   Article

   Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

   Insert

   Sp dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   sfGFP

   Expression

   Mammalian

   Promoter

   CMV-TO

   Available Since

   May 1, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV6-AN-HA_WT POLH

    Plasmid

    #201671

    Purpose

    Expression of WT DNA polymerase eta with an N-terminal HA tag in mammalian cells

    Depositor

    Roger Woodgate

    Article

    McIntyre et al Nucleic Acids Res. 2013 Feb 1;41(3):1649

    Insert

    Polymerase eta (POLH Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Coding sequence has been optimised for expression…

    Promoter

    CMV

    Available Since

    June 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. MCP/PCP RNA Lantern

    Plasmid

    #226095

    Purpose

    RNA tracking

    Depositor

    Jennifer Prescher

    Article

    Halbers et al Nat Commun. 2024 Nov 18;15(1):9992. doi:

    Insert

    NLS-HA-MS2-SmBiT-IRES-PP7-LgBiT-FLAG
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Jan. 8, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLVE-eGFP

    Plasmid

    #52581

    Purpose

    Lentiviral transfer vector driving expression of eGFP in mammalian cells

    Depositor

    Charles Gersbach

    Article

    Brunger et al Proc Natl Acad Sci U S A. 2014 Mar 4;111

    Insert

    eGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1alpha

    Available Since

    Jan. 6, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. IGF2BP1

    Plasmid

    #155676

    Purpose

    For use in RBP tethering screen

    Depositor

    Eugene Yeo

    Article

    Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Tags

    V5-MCP

    Expression

    Mammalian

    Mutation

    none

    Promoter

    EF-1a

    Available Since

    Sept. 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFRT/TO/HIS/FLAG/HA-PRDX1

    Plasmid

    #38086

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    PRDX1 peroxiredoxin 1 (PRDX1 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET-Cas9-NLS-6xHis

    Plasmid

    #62933

    Purpose

    Expression of Cas9-NLS-6xHis in bacterial cells

    Depositor

    David Liu

    Article

    Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

    Insert

    Cas9-NLS-6xHis
    

    Tags

    6xHis tag and NLS

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRel-Hyg

    Plasmid

    #227582

    Purpose

    Release plasmid with Cas9 working cassette, hygromycin resistance, and pSG5 replicon

    Depositor

    Chengzhang Fu

    Article

    Xie et al Science. 2024 Dec 13;386(6727):eabq7333.

    Insert

    Cas9
    

    Use

    CRISPR

    Available Since

    Dec. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLKO.1 - TRC mTagBFP2

    Plasmid

    #191566

    Purpose

    Expresses an shRNA and mTagBFP2

    Depositor

    Timothy Ryan

    Article

    Kokotos et al Sci Adv. 2024 Aug 23;10(34):eadn6016. do

    Type

    Empty backbone

    Use

    Lentiviral and RNAi

    Expression

    Mammalian

    Promoter

    U6 for shRNA

    Available Since

    Oct. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. NES-EGFP-PH-ARNO2G-I303Ex2

    Plasmid

    #116868

    Purpose

    PIP3 biosensor

    Depositor

    Gerry Hammond

    Article

    Goulden et al J Cell Biol. 2019 Mar 4;218(3):1066-1079

    Insert

    CYTH2 (CYTH2 Human)
    

    Tags

    EGFP and X.leavis map2k1.L(32-44)

    Expression

    Mammalian

    Mutation

    amind acids 252-399, isoleucine 303 changed to gl…

    Available Since

    Oct. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pML104-KanMx4

    Plasmid

    #83476

    Purpose

    Expresses Cas9 and contains guide RNA expression cassette with BclI-SwaI cloning sites for guide sequence cloning; Contains KanMx4 marker for yeast transformation.

    Depositor

    Patricia Wittkopp

    Insert

    KanMX4
    

    Expression

    Yeast

    Available Since

    Oct. 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEGFR-PATagRFP

    Plasmid

    #31950

    Depositor

    Vladislav Verkhusha

    Article

    Subach et al J Am Chem Soc. 2010 May 12;132(18):6481-

    Insert

    PATagRFP
    

    Tags

    EGF receptor (EGFR)

    Expression

    Mammalian

    Mutation

    R69S/F84W/Q139K/A147P/N148S/M151K/Y153K/S165V/H20…

    Promoter

    CMV

    Available Since

    Oct. 28, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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