159,617 results

1. QPM-sgR (pTaU3)

   Plasmid

   #140448

   Purpose

   For sgRNA expression in monocotyledons protoplasts

   Depositor

   Caixia Gao

   Article

   Lin et al Nat Biotechnol. 2020 May;38(5):582-585.

   Insert

   TaU3-sgRNA
   

   Use

   CRISPR

   Tags

   Expression

   Plant

   Mutation

   Promoter

   TaU3

   Available since

   May 5, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG Cas9-2A-Citrine

   Plasmid

   #92358

   Purpose

   CAG-driven ubiquitous expression of Cas9. Contains 2A-Citrine reporter. For CRISPR mediated gene knockouts in chicken embryos.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET LIC cloning vector (2A-T)

   Plasmid

   #29665

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 9, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRDA_867

   Plasmid

   #231109

   Purpose

   A>G base editor

   Depositor

   David Root, John Doench

   Insert

   ABE8e-nCas9-SpG-HA
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   D10A

   Promoter

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. At2S3:RUBY

   Plasmid

   #160906

   Purpose

   RUBY under the control of At2S3 promoter/marker for Arabidopsis transgenes

   Depositor

   Yunde Zhao

   Article

   He et al Hortic Res. 2020 Sep 19;7:152. doi: 10.1

   Insert

   RUBY and At2S3 promoter
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   At2S3

   Available since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. PB-CMV-MCS-Puro-EGFP

   Plasmid

   #219795

   Purpose

   Mammalian expression plasmids using piggyBac Transposon System

   Depositor

   Keiichi Nakayama

   Type

   Empty backbone

   Use

   Piggybac transposon

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA 3.1 Flag-rat-PTP1B

   Plasmid

   #35988

   Depositor

   Anna Huttenlocher

   Article

   Cortesio et al J Cell Biol. 2008 Mar 10;180(5):957-71.

   Insert

   protein tyrosine phosphatase 1B (Ptpn1 Rat)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 1, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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8. SPYtag-GFPnanobody

   Plasmid

   #214837

   Purpose

   This plasmid encodes a protein (SPYtag-GFPnanobody) that is used for generating functionalized magnetic beads for MagIC-cryo-EM, a method for single-particle cryo-EM analysis on magnetic beads.

   Depositor

   Hironori Funabiki

   Article

   Arimura et al bioRxiv 2024.01.21.576499.

   Insert

   SPYtag-GFPnanobody
   

   Use

   Tags

   His-TEV-SUMO-

   Expression

   Bacterial

   Mutation

   Promoter

   T5

   Available since

   March 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCas9-CR4

   Plasmid

   #62655

   Purpose

   Cas9 nuclease under control of pTet promoter with ssrA tag and constitutive tetR

   Depositor

   Kristala Prather

   Article

   Reisch et al Sci Rep. 2015 Oct 14;5:15096. doi: 10.10

   Insert

   Cas9 nuclease
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3131

    Plasmid

    #144607

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    NR5A2 (NR5A2 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-lyso-pHoenix

    Plasmid

    #70112

    Purpose

    Expression of lyso-pHoenix in mammalian cells for optogenetic acidification of lysosomes

    Depositor

    Christian Rosenmund

    Article

    Rost et al Nat Neurosci. 2015 Nov 9. doi: 10.1038/n

    Insert

    lyso-pHoenix (Cd63 Halorubrum sodomense, Entacmaea quadricolor, Aequorea victoria, Mouse, Rat)
    

    Use

    Tags

    Arch3, H+/K+ ATPase beta-subunit, HA-Tag, mKate2,…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCH67 (crRNA expression vector)

    Plasmid

    #217337

    Purpose

    CROP-seq crRNA expression vector with GFP and puro marker

    Depositor

    Luke Gilbert

    Article

    Hsiung et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Insert

    AsDR-filler-AsDR8, GFP-puro
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    hU6

    Available since

    March 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. modRNAc1-Cas9-2A-p53DD

    Plasmid

    #216389

    Purpose

    this vector can be used to make modRNA expressing Cas9 and p53DD

    Depositor

    Xiaojun Lian

    Article

    Haideri et al Stem Cell Reports. 2024 May 14;19(5):744

    Insert

    Cas9-2A-p53DD
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-ArcLight-ST

    Plasmid

    #182318

    Purpose

    Fluorescent voltage indicator with left-shifted voltage-dependency

    Depositor

    Rafael Yuste

    Article

    Bando et al Nat Commun. 2021 Dec 10;12(1):7229. doi:

    Insert

    ArcLight-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Mutation in an amino acid Q217E of ArcLight-MT

    Promoter

    CAG

    Available since

    April 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV5 WT KSR1

    Plasmid

    #25970

    Depositor

    Rob Lewis

    Article

    Joneson et al J Biol Chem. 1998 Mar 27. 273(13):7743-8

    Insert

    Kinase suppressor of Ras 1 (Ksr1 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 13, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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16. TFORF2987

    Plasmid

    #144463

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    RORA (RORA Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pENN.AAV.CamKII.GCaMP6f.WPRE.SV40

    Plasmid

    #100834

    Purpose

    AAV expression of ultrasensitive protein calcium sensor from CamKII promoter

    Depositor

    James M. Wilson

    Has Service

    AAV1, AAV5, and AAV9

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    GCaMP3-T302L R303P A317E D380Y T381R S383T R392G

    Promoter

    CamKII

    Available since

    March 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-GFAP-hM4D(Gi)-mCherry

    Plasmid

    #50479

    Purpose

    Gi-coupled hM4D DREADD fused with mCherry under the control of GFAP promoter

    Depositor

    Bryan Roth

    Has Service

    AAV5

    Insert

    hM4D(Gi)-mCherry
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mutation

    See supplemental documents for DREADD mutations

    Promoter

    GFAP

    Available since

    April 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

    Plasmid

    #117484

    Purpose

    Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

    Depositor

    Bo Huang

    Article

    Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

    Insert

    SpyCatcher002-6aa-sfCherry2(1-10)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 29, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pHR_SFFv_4D5-WT-Highest-CAR_RHL004

    Plasmid

    #164826

    Purpose

    Lentiviral vector for constitutive expression of AntiHER2 4D5-WT-Highest CAR

    Depositor

    Wendell Lim

    Article

    Hernandez-Lopez et al Science. 2021 Mar 12;371(6534):1166-1171

    Insert

    AntiHER2 4D5-WT Chimeric Antigen receptor (CD8alphaTM-41BB-CD3z)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    June 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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