177,653 results
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Plasmid#67640PurposeURA3 vector for cloning guide sequences using BclI-SwaI sites into guide RNA expression cassetteDepositorInsertsingle guide RNA expression cassette
UseCRISPRExpressionYeastPromoterpSNR52Available sinceAug. 31, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA5FRT-EF-Pdgfrbeta-EGFPN
Plasmid#66790PurposeExpression of murine PDGFRbeta tagged with GFP under the control of EF1a promoterDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertPDGFRbeta (Pdgfrb Mouse)
TagsC-term EGFPExpressionMammalianPromoterEF1aAvailable sinceAug. 18, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP_CD47_SU
Plasmid#65473PurposeGFP tagged CD47 with its own short 3'UTRDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsSignal peptide of CD47
GFP
CD47 coding region continued and short 3’UTR.
ExpressionMammalianAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
Str-KDEL_neomycin
Plasmid#65306PurposeLuminal ER hook only (RUSH system)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertStreptavidin fused to a signal sequence at 5' end and to a KDEL motif at 3' end
TagsKDEL motif and Signal SequenceExpressionMammalianPromoterCMVAvailable sinceJune 12, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
CRISPR-SP-Cas9 reporter
Plasmid#62733PurposeMammalian CRISPR-SP-Cas9 reporterDepositorInsertCRISPR-SP-Cas9 target seq + tdTomato (out of frame)
UseCRISPR and LentiviralTagsCRISPR-SP-Cas9 target seq (hAAVS1 locus) and HA t…ExpressionMammalianPromoterEF1-AlphaAvailable sinceApril 24, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSIN4-EF1a-GATA2-IRES-Puro
Plasmid#61063Purposelentiviral vector for GATA2DepositorAvailable sinceMarch 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pQE-80L MBP-SspB Nano
Plasmid#60409PurposeE. coli expression of SspB NanoDepositorInsertSspB nano
TagsHis-MBP-TEVExpressionBacterialMutationY11K and A15EAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
gRNA-eGFP-Reporter
Plasmid#60719PurposeExpresses guide RNA used to activate pGL3-Basic-8x-gRNA-eGFP reporter.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertgRNA (5'-AAAGGTCGAGAAACTGCAAA-3')
UseCRISPRExpressionMammalianPromoterU6 and mouse U6Available sinceJan. 30, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pCS2-Gal4ff.zf1
Plasmid#61392PurposemRNA synthesis of Gal4ff codon modified for zebrafishDepositorInsertGal4ff.zf1
UseZebrafish expressionPromotersp6Available sinceJan. 28, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC-ZEAXipi
Plasmid#53287PurposeContains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and thereby produces zeaxanthin in E. coliDepositorInsertcrtE, idi, crtY, crtI, crtB, crtZ
UseLow copy number bacterial cloning vectorPromoterendogenous promotersAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pUltra-puro-RTTA3
Plasmid#58750Purposesilence doxycyclin inducible pUltra-doxDepositorInsertReverse TET-Transactivator 3
UseLentiviralExpressionMammalianMutationloxP5171PromoterUbiquitin C promoterAvailable sinceOct. 6, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRExpressionMammalianAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Sequestosome1-N-18
Plasmid#55132PurposeLocalization: Membrane Scaffold, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSequestosome1 (SQSTM1 Human)
TagsmCherryExpressionMammalianPromoterCMVAvailable sinceAug. 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FRP793_insul-(lexA-box)4-PminCYC1-Citrine-TCYC1
Plasmid#58434PurposeInduces Citrine expression from a promoter containing 4 lexA boxes in yeast cellsDepositorInsertCitrineA206K
ExpressionYeastPromoterinsul-(lexA-box)4-PminCYC1Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP-C3-Sec5
Plasmid#53756PurposepEGFP-C3 plasmid that expresses Sec5DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec5 (EXOC2 Human)
TagsGFPExpressionMammalianAvailable sinceJuly 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mEos2-C1
Plasmid#54510PurposeLocalization: C1 Cloning Vector, Excitation: 505 / 569, Emission: 516 / 581DepositorTypeEmpty backboneTagsmEos2ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityIndustry, Academic Institutions, and NonprofitsCited by -
pcDNA3.1 3xFlag IFIT3
Plasmid#53553Purposemammalian expression of IFIT3DepositorAvailable sinceMay 29, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
DRH322: AAV-CaMKIIa-QuasAr2-mO2
Plasmid#51696PurposeAAV production of QuasAr2 fluorescent reporterDepositorInsertQuasAr2-mOrange
UseAAVTagsmOrange2PromoterCaMKIIaAvailable sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits only -
pENTR-AtMIR390a-B/c
Plasmid#51778PurposeGateway-compatible entry vector for direct cloning of artificial microRNAs into Arabidopsis thaliana MIR390a precursorDepositorPublicationInsertAtMIR390a-B/c
UseGateway CloningMutationA. thaliana MIR390a precursor sequence including …Available sinceApril 1, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBIR-GFP
Plasmid#49807PurposeIn vivo GFP based system to measure break induced replication (BIR) repair efficiency. expression of I-SceI generates a double strand break that when repaired by BIR mechanism will restore GFP.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertGFP
ExpressionMammalianPromoterbeta-actinAvailable sinceJan. 10, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by