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pDONR223-BMPR1B

Plasmid

#23638

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

BMPR1B (BMPR1B Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only
pDONR223-CSNK2A1

Plasmid

#23678

Depositor

William Hahn, David Root

Article

Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

Insert

CSNK2A1 (CSNK2A1 Human)

Use

Gateway Cloning

Available Since

July 30, 2010

Availability

Academic Institutions and Nonprofits only

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pLD-puro-CnVA

Plasmid

#24587

Depositor

Jason Moffat

Article

Mak et al Mol Cell Proteomics. 2010 May;9(5):811-2

Insert

Gateway(TM) cassette

Use

Lentiviral

Tags

VA

Expression

Mammalian

Available Since

July 14, 2010

Availability

Academic Institutions and Nonprofits only
pMCS.71/2272.Hygro

Plasmid

#22731

Depositor

Mark Magnuson

Article

Chen et al Dis Model Mech. 2011 Jul;4(4):537-47. do

Insert

Lox71-FRT-PGK-hygro-FRT-Lox2272

Use

Cre/Lox

Mutation

This is a basal exchange vector for RMCE. The vec…

Available Since

Dec. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pWZL Neo Myr Flag MAPK7

Plasmid

#20527

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAPK7 (MAPK7 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag RPS6KA5

Plasmid

#20622

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

RPS6KA5 (RPS6KA5 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag PFKL

Plasmid

#20657

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PFKL (PFKL Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag AURKA

Plasmid

#20427

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

AURKA (AURKA Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pET3d-E2-25K-C170S

Plasmid

#18892

Depositor

Cecile Pickart

Article

Raasi et al Methods Mol Biol. 2005;301:47-55. doi: 1

Insert

Ubiquitin conjugating enzyme E2-25K (UBE2K Most likely H. sapiens, Human)

Expression

Bacterial

Mutation

Cysteine 170 is replaced with a Serine residue.

Available Since

Sept. 4, 2008

Availability

Academic Institutions and Nonprofits only
pBabe-mHSF1-zeo

Plasmid

#17755

Depositor

Susan Lindquist

Article

Dai et al Cell. 2007 Sep 21. 130(6):1005-18.

Insert

HSF1 (Hsf1 Mouse)

Use

Retroviral

Expression

Mammalian

Available Since

April 14, 2008

Availability

Academic Institutions and Nonprofits only
pGEX-5X1 Ash2L full

Plasmid

#15549

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

Ash2L (ASH2L Human)

Tags

GST

Expression

Bacterial

Available Since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
pGEX2T E7

Plasmid

#13634

Depositor

Karl Munger

Article

Wu et al J Virol. 1993 Apr . 67(4):2402-7.

Insert

HPV 16 E7 (E7 Human)

Tags

GST

Expression

Bacterial

Available Since

March 2, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-4T-2 RanGAP

Plasmid

#13379

Depositor

Mary Dasso

Article

Joseph et al J Cell Biol. 2002 Feb 18. 156(4):595-602

Insert

RanGAP (RANGAP1 Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 16, 2006

Availability

Academic Institutions and Nonprofits only
pYES2-Ub-R-GFP

Plasmid

#11953

Depositor

Nico Dantuma

Article

Heessen et al FEBS Lett. 2003 Dec 4. 555(2):397-404.

Insert

Ub-R-GFP

Expression

Yeast

Mutation

Ubiquitin fused to N-terminus of GFP. Arginine at…

Available Since

May 17, 2006

Availability

Academic Institutions and Nonprofits only

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pET23b-Casp3 C163A-His

Plasmid

#11822

Depositor

Guy Salvesen

Article

Zhou et al J Biol Chem. 1997 Mar 21. 272(12):7797-8

Insert

Caspase 3 catalytic mutant (CASP3 Human)

Tags

6xHis

Expression

Bacterial

Mutation

C163A

Available Since

May 12, 2006

Availability

Academic Institutions and Nonprofits only
pGJK_His-SUMO-LbuCas13a

Plasmid

#172488

Purpose

Bacterial expression of codon-optimized His-SUMO-LbuCas13a.

Depositor

Jennifer Doudna

Article

Liu et al Nat Chem Biol. 2021 Sep;17(9):982-988. d

Insert

bdSUMO-LbuCas13a (codon-optimized)

Tags

His6 and bdSUMO

Expression

Bacterial

Promoter

T7 promoter and lac operator

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-DIO-EF1α-mKate2-biPAC

Plasmid

#169127

Purpose

AAV plasmid containing a mKate2 and a light-gated adenylate cyclase biPAC

Depositor

Georg Nagel

Article

Zhang et al Nature. 2021 Sep;597(7875):245-249. doi:

Insert

mKate2-biPAC

Use

AAV and Cre/Lox

Tags

mKate2

Expression

Mammalian

Mutation

codon optimized to mouse

Promoter

EF1a

Available Since

Jan. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-rtTA

Plasmid

#163601

Purpose

Expresses rtTA in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

rtTA

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRmCerulean3

Plasmid

#59149

Purpose

ORF encoding p38 KTR mCerulean3 flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Available Since

Sept. 18, 2014

Availability

Academic Institutions and Nonprofits only

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tdTomato-anti-AlfaTag nanobody

Plasmid

#171819

Purpose

Bacterial expression vector for production of fluorescent anti-AlfaTag nanobody

Depositor

Ikuo Wada

Article

Matsumoto et al J Biol Chem. 2021 Jul;297(1):100851. doi

Insert

tdTomato-anti-AlfaTag nanobody

Tags

6xHis and SEP tag (6xD)

Expression

Bacterial

Available Since

July 12, 2022

Availability

Academic Institutions and Nonprofits only

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