We narrowed to 16,382 results for: Jun

1. pTargetF-FRT

   Plasmid

   #113637

   Purpose

   Derivative of pTargetF with gRNA targeting FRT site

   Depositor

   Olivier Lichtarge

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   sgRNA-FRT
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   pJ23119

   Available since

   Sept. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKDsgRNA-FRT

   Plasmid

   #113398

   Purpose

   expresses gRNA for Cas9 FRT targetting

   Depositor

   Jan Michiels

   Article

   Swings et al Nat Commun. 2018 Jun 8;9(1):2231. doi: 1

   Insert

   exo, beta, gam, sgRNA-FRT
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Aug. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLentiPGK Puro DEST JNKKTRClover

   Plasmid

   #59151

   Purpose

   Lentiviral vector to express JNK KTR mClover under PGK promoter (With Puromycin Resistance)

   Depositor

   Markus Covert

   Article

   Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

   Insert

   JNK Kinase Translocation Reporter (MAPK8 Mouse, Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   PGK

   Available since

   Sept. 18, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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4. Myc-E-Syt1

   Plasmid

   #66833

   Purpose

   Mammalian Expression, Bacterial Expression

   Depositor

   Pietro De Camilli

   Article

   Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

   Insert

   E-Syt1 (ESYT1 Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRRL-SV40-Puro-CMV-mCherry-EGFP-LC3B

   Plasmid

   #223712

   Purpose

   Expresses autophagy flux LC3B fluorescence reporter

   Depositor

   Benjamin Wolozin

   Article

   Ortiz et al bioRxiv [Preprint]. 2024 Jun 27:2023.12.

   Insert

   LC3B (MAP1LC3B Human, Synthetic)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   mCherry::EGFP::LC3B

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 28, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAG416GAL-EGFP

   Plasmid

   #166140

   Purpose

   EGFP expression under the control of the Galactose-inducible promoter yeast. Contains CEN/ARS element for low copy within yeast.

   Depositor

   Daniel Jarosz

   Article

   Harel et al Cell Rep. 2024 Jun 25;43(6):112787. doi:

   Insert

   EGFP
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL1

   Available since

   May 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX Zap70(NC)-SH2

   Plasmid

   #46535

   Depositor

   Bruce Mayer

   Article

   Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

   Insert

   Zap70(NC) (ZAP70 Human)
   

   Use

   Tags

   GST and PreScission

   Expression

   Bacterial

   Mutation

   contains amino acids 1-268

   Promoter

   Tac

   Available since

   Aug. 12, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-hysn-flex-dsRed-shscramble

   Plasmid

   #71383

   Purpose

   Expresses scramble shRNA Cre-dependently by flex switch

   Depositor

   William Wisden

   Article

   Yu et al Neuron. 2015 Jun 17. pii: S0896-6273(15)

   Insert

   human synapsin promoter-flex switch-dsRed-shscramble
   

   Use

   AAV, Cre/Lox, and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJMP2

   Plasmid

   #79874

   Purpose

   Bacillus subtilis sgRNA expression vector; integrates into amyE

   Depositor

   Jason Peters, Stanley Qi, Carol Gross

   Article

   Peters et al Cell. 2016 Jun 2;165(6):1493-506. doi: 1

   Insert

   sgRNA RR1
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   veg

   Available since

   Sept. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pN1-RnKIF5C(1-560)-Halo-Flag

    Plasmid

    #188071

    Purpose

    Mammalian cell expression of truncated version (aa 1-560) of rat kinesin-1 KIF5C tagged with Halo and Flag tags

    Depositor

    Kristen Verhey

    Article

    Budaitis et al Curr Biol. 2022 Jun 6;32(11):2416-2429.e

    Insert

    rat KIF5C (Kif5c Rat)
    

    Use

    Tags

    Flag and Halo

    Expression

    Mammalian

    Mutation

    aa1-560

    Promoter

    CMV

    Available since

    Aug. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MSP712

    Plasmid

    #65768

    Purpose

    Bacterial expression plasmid for Sp-dCas9 & sgRNA (need to clone in spacer into BsaI sites): T7-humanSpdCas9(D10A/H840A)-T7-BsaIcassette-Sp-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    mammalian codon-optimized Streptococcus pyogenes dCas9 (D10A/H840A)-NLS-3XFlag, and SpCas9 gRNA
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Bacterial

    Mutation

    D10A and H840A mutations in Cas9

    Promoter

    T7 (x2)

    Available since

    June 24, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pC001 - huLshC2C2-MBP for bacterial expression

    Plasmid

    #79150

    Purpose

    Expresses human codon-optimized LshC2c2 for purification in E. coli

    Depositor

    Feng Zhang

    Article

    Abudayyeh et al Science. 2016 Jun 2. pii: aaf5573.

    Insert

    C2c2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28-his-3C_Tau352

    Plasmid

    #100092

    Purpose

    Expresses human full-length fetal tau protein with the N-terminal 3C-cleavable His6 tag

    Depositor

    Nikolai Sluchanko

    Article

    Tugaeva et al PLoS One. 2017 Jun 2;12(6):e0178933. doi

    Insert

    Fetal microtubule-associated protein tau (MAPT Human)
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    wild type sequence

    Promoter

    T7

    Available since

    Sept. 22, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-nEF-ChRmine-mScarlet

    Plasmid

    #137158

    Purpose

    Viral expression of ChRmine-p2a-mScarlet

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Has Service

    AAV8

    Insert

    ChRmine-p2a-mScarlet
    

    Use

    AAV

    Tags

    Expression

    Mutation

    n/a

    Promoter

    nEF

    Available since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAS_4xhybPylT A41AA C55A FLAG-G1 PylRS Y125A

    Plasmid

    #154773

    Purpose

    plasmid with 4xhybrid PylT cassette (Mx1201 G1 PylT hybrid mutant A41AA C55A) and G1 PylRS Y125A for amber suppression in transient or stable, piggybac-mediated, integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep. 2020 Jun 23;31(12):107811. doi

    Insert

    G1 PylRS (MJ_RS07805 methanogenic archaeon mixed culture ISO4-G1)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y125A in G1PylS, hybrid PylT with A41AA and C55A …

    Promoter

    EF1

    Available since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMB1_MmPylRS_MmtRNA-Pyl-opt(UGA)

    Plasmid

    #174515

    Purpose

    Recoded single aaRS/tRNA plasmid with MmPylRS and MmtRNA-Pyl-opt with the ASL from the serT gene (UGA anticodon)

    Depositor

    Jason W Chin

    Article

    Robertson et al Science. 2021 Jun 4;372(6546):1057-1062.

    Inserts

    Mm PylS
    MmtRNA-Pyl-opt(UGA)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    ASL from the serT gene (UGA anticodon) and recoded

    Promoter

    GlnS and lpp

    Available since

    Oct. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pHR-BTLA-mGFP

    Plasmid

    #180791

    Purpose

    Expression of human BTLA fused to mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLA (BTLA Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Expression

    Mutation

    Promoter

    SFFV

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. BAP1 C91A

    Plasmid

    #81025

    Purpose

    Retroviral vector containing human BAP1 C91A (catalytically inactive mutant) and Thy1.1 selection marker

    Depositor

    Anjana Rao

    Article

    Balasubramani et al Nat Commun. 2015 Jun 22;6:7307. doi: 10.

    Insert

    BAP1 (BAP1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    BAP1 C91A (point mutation, catalytically inactive)

    Promoter

    5’LTR

    Available since

    Nov. 17, 2016

    Availability

    Academic Institutions and Nonprofits only

19. pSicoR-Ef1a-mCh

    Plasmid

    #31847

    Purpose

    Cre-regulated lentiviral shRNA vector. Cre addition causes both mCherry and shRNA to be recombined out of the construct, turning OFF shRNA expression.

    Depositor

    Bruce Conklin

    Article

    Salomonis et al Proc Natl Acad Sci U S A. 2010 Jun 8;107

    Type

    Empty backbone

    Use

    Cre/Lox, Lentiviral, and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 for shRNA; Ef1alpha for mCherry

    Available since

    Jan. 3, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. p5E-3x125bp-mnx1

    Plasmid

    #169043

    Purpose

    5' entry vector for Gateway cloning for expression in spinal motor neurons using 3 tandem repeats of the zebrafish mnx1 enhancer.

    Depositor

    Paola Arlotta

    Article

    Harris et al Dev Cell. 2020 Jun 8;53(5):577-588.e7. d

    Insert

    Mnx1 enhancer (3x) (mnx1 Zebrafish)
    

    Use

    Gateway entry cloning vector

    Tags

    Expression

    Mutation

    Promoter

    3xMnx1

    Available since

    Jan. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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