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Plasmid#103690PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-630 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only
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LSB-hsa-miR-633
Plasmid#103691PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-633 target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LSB-hsa-miR-135b-5p
Plasmid#103229PurposeUsed to sense miRNA activity using fluorescence based tools. hsa-miR-135b-5p target in 3' UTR of of mKate. miRNA activity can be measured by the repression of mKate2 relative to EBFP2 fluorescent proteins . Plasmid is inherently unstable; please see Depositor CommentsDepositorInserthsa-miR-135b-5p target (MIR135B Human)
UseSynthetic BiologyExpressionMammalianPromoterEF-1aAvailable SinceNov. 26, 2018AvailabilityAcademic Institutions and Nonprofits only -
LLP338 pEF1a-DACH1-1-SunTag
Plasmid#100942PurposeTALE vector with DACH1-1 insert fused to SunTagx10, driven by EF1a, with 3xHA tag, no PURO geneDepositorInsertTALE target sequence for DACH1, SunTag array (10xGCN4)
UseTALENTags3xHAExpressionMammalianAvailable SinceJune 21, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGEX Ptk70-SH2
Plasmid#46473DepositorInsertPtk70 (Srms Mouse)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 123-236PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Rin2-SH2
Plasmid#46475DepositorInsertRin2 (RIN2 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 94-193PromoterTacAvailable SinceAug. 1, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Bks-SH2
Plasmid#46406DepositorInsertBks (STAP2 Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 141-266PromoterTacAvailable SinceJuly 12, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nap4-SH2
Plasmid#46456DepositorInsertNap4 (SOCS7 Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 395-514PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Nsp1-SH2
Plasmid#46459DepositorInsertNsp1 (SH2D3A Human)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 3-119PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Slnk-SH2
Plasmid#46502DepositorInsertSlnk (Sh2d6 Mouse)
TagsGST and PreScissionExpressionBacterialMutationcontains amino acids 199-323PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pGEX Socs7-SH2
Plasmid#46509DepositorInsertSocs7 (SOCS4 Human)
TagsAviTag, GST, and PreScissionExpressionBacterialMutationcontains amino acids 280-386PromoterTacAvailable SinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAG415-ADH1p-HA-rap1-K9R
Plasmid#84531PurposeThis plasmid can be used to express HA-tagged Rap1-K9R from the ADH promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
TagsHAExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterADH1Available SinceJan. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3NR
Plasmid#84533PurposeThis plasmid can be used to express EGFP-tagged Rap1K3NR from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K3CR
Plasmid#84534PurposeThis plasmid can be used to express EGFP-tagged Rap1K3CR from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K9R
Plasmid#84535PurposeThis plasmid can be used to express EGFP-tagged Rap1K9R from the GDP promoter in yeastDepositorInsertRAP1 (RAP1 Budding Yeast)
TagsEGFPExpressionYeastMutationK(43,61,69,117,240,246,527,582,651)RPromoterGPDAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAG415-GPDp-EGFP-rap1-K651R
Plasmid#84536PurposeThis plasmid can be used to express EGFP-tagged Rap1-K651R from the GDP promoter in yeastDepositorAvailable SinceDec. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
CSII-IDR2-AGMpygTRIM5-FOS
Plasmid#79064PurposeExpress African green monkey pygerythrus TRIM5alpha in mammalan cellsDepositorInsertTRIM5alpha(African green monkey pygerythrus)-FOS
TagsOneSTrEP-FLAGExpressionMammalianPromoterCMVAvailable SinceMarch 20, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUASp YFP Rab11 CA
Plasmid#53472DepositorAvailable SinceJune 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUASP YFP Rab32 DN
Plasmid#37739DepositorInsertRab32 (Rab32 Fly)
UseDrosophilaTagsYFPExpressionInsectMutationT33N (dominant negative)PromoterGAL4Available SinceNov. 20, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC E254D
Plasmid#100033PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 390 nm.DepositorInsertRhoGC E254D
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationE254DPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only