We narrowed to 15,732 results for: LEA

1. kiCAP-AAV-MyoAAV4B

   Plasmid

   #224449

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4B, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYTSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. kiCAP-AAV-MyoAAV4D

   Plasmid

   #224451

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4D, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDHGVL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. kiCAP-AAV-MyoAAV4C

   Plasmid

   #224450

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 4C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYTSM insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. kiCAP-AAV-MyoAAV3C

   Plasmid

   #224444

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3C, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYSSV insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. kiCAP-AAV-MyoAAV3B

   Plasmid

   #224443

   Purpose

   Rep/Cap plasmid for the production of MyoAAV 3B, a muscle-tropic AAV capsid in mice and macaques.

   Depositor

   Pardis Sabeti

   Article

   Tabebordbar et al Cell. 2021 Sep 16;184(19):4919-4938.e22.

   Insert

   AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
   

   Use

   AAV

   Mutation

   RGDYSGL insert between amino acids 588 and 589 of…

   Promoter

   p41

   Available Since

   Sept. 17, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pelB-MBP-BB0238-ss

   Plasmid

   #137046

   Purpose

   E. coli expression clone (T7lac promoter) for mature BB0238 (aa 21-256) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

   Depositor

   Debra Hansen

   Article

   Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

   Insert

   AAC66635.2 (BB_0238 Borrelia burgdorferi B31)
   

   Tags

   PelB signal peptide + His10 + maltose binding pro…

   Expression

   Bacterial

   Mutation

   mature BB0238: lacks the BB0238 signal peptide

   Promoter

   T7lac

   Available Since

   April 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNGVL4a-hCRTmE6mE7mL2

   Plasmid

   #104153

   Purpose

   Expresses human calreticulin fused to MmuPV1 E6, MmuPV1 E7 and MmuPV1 L2 residues 11-210 in mammalian cells

   Depositor

   Richard Roden

   Article

   Jiang et al J Virol. 2017 Jul 12;91(15). pii: e00699

   Insert

   hCRTmE6mE7mL2 11-210 (CALR, E6, E7, L2 Human, MmuPV1)
   

   Expression

   Mammalian

   Mutation

   residues 11 to 200 of the late protein L2

   Promoter

   CMV

   Available Since

   Dec. 18, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. Unfused click editor construct; PCV2-EcKlenow fusion - pCMV-T7-PCV2-EcKlenow (JO1421)

   Plasmid

   #217804

   Purpose

   Unfused click editor construct expressing PCV2-EcKlenow, expressed from CMV or T7 promoters.

   Depositor

   Benjamin Kleinstiver

   Article

   Ferreira da Silva et al Nat Biotechnol. 2024 Jul 22. doi: 10.103

   Insert

   PCV2-linker-EcKlenow(-exo)-BPNLS
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   EcKlenow(-exo;D355A/E357A)

   Promoter

   CMV and T7

   Available Since

   Sept. 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDule-3-nitroTyrosine (A7)

   Plasmid

   #174078

   Purpose

   Plasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the third generation Mj 3NY (A7) synthetase and cognate amber suppressing tRNA in Ecoli.

   Depositor

   Ryan Mehl

   Article

   Beyer et al J Mol Biol. 2020 Jul 24;432(16):4690-470

   Insert

   3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
   

   Expression

   Bacterial

   Mutation

   Y32H H70T D158H I159A L162R

   Promoter

   GlnRS (constitutive)

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-DroshaS300E/S302D

    Plasmid

    #62531

    Purpose

    Serine 300 of Drosha is mutated to glutamic acid and serine 302 is mutated to aspartic acid

    Depositor

    Bharat Ramratnam

    Article

    Tang et al Nucleic Acids Res. 2010 Oct;38(19):6610-

    Insert

    human Drosha with serine 300 changed to glutamic acid and serine 302 changed to aspartic acid (DROSHA Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    changed serine 300 to glutamic acid and serine 3…

    Available Since

    March 20, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pOTTC1442 - pAAV CMV-IE Nuc-iRFP-2A-iCre

    Plasmid

    #112683

    Purpose

    An AAV vector that expresses a nuclear-localized iRFP713 reporter and improved Cre recombinase

    Depositor

    Brandon Harvey

    Article

    Back et al Neuron. 2019 Feb 8. pii: S0896-6273(19)3

    Insert

    iRFP713
    

    Use

    AAV

    Tags

    2A-iCre and Nuclear localization signal

    Promoter

    CMV-IE

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. tetO-NR2F1

    Plasmid

    #170698

    Purpose

    doxycycline-inducible overexpression of NR2F1

    Depositor

    Paul Burridge

    Article

    Romero-Tejeda et al J Mol Cell Cardiol. 2023 Jul 6:S0022-282

    Insert

    nuclear receptor subfamily 2 group F member 1 (NR2F1 Human)
    

    Use

    Lentiviral; Doxycycline inducible

    Expression

    Mammalian

    Mutation

    deletion of 36G- please see depositor comments

    Promoter

    TRE promoter, Tet-ON

    Available Since

    July 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pRetroX-PTuner DD-M.EcoGII-v5-Centromeric protein C

    Plasmid

    #122085

    Purpose

    Express M.EcoGII fused to CENPC with destabilization domain - inducible

    Depositor

    Laure Crabbe

    Article

    Sobecki et al Cell Rep. 2018 Dec 4;25(10):2891-2903.e5

    Insert

    DD-linker-M.EcoGII-V5-CENPC (CENPC Human, E.coli)
    

    Use

    Retroviral

    Tags

    V5 tag on Nter of CENPC

    Expression

    Bacterial and Mammalian

    Mutation

    L341F, R930L (see comments below)

    Available Since

    March 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBABE-α-Syn-nYFP

    Plasmid

    #92203

    Purpose

    Retroviral overexpression vector for α-Syn bimolecular fluorescence complementation (BiFC)

    Depositor

    Huda Zoghbi

    Article

    Rousseaux et al Elife. 2016 Oct 25;5. pii: e19809. doi:

    Insert

    SNCA (SNCA Human)
    

    Use

    Retroviral

    Tags

    YFP(1-155)

    Expression

    Mammalian

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. Cherry-HA-KCC2

    Plasmid

    #104077

    Purpose

    To visualize the expression and localization of potassium-chloride cotransporter type 2 (KCC2)

    Depositor

    Igor Medyna

    Article

    Kahle et al EMBO Rep. 2014 Jul;15(7):766-74. doi: 10

    Insert

    KCC2 (b isoform) (Slc12a5 Rat)
    

    Tags

    mCherry-HA tag

    Expression

    Mammalian

    Mutation

    EcoR1 site in rat KCC2 was removed by silent muta…

    Promoter

    UbC

    Available Since

    Feb. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pBactin-Nedd1-mOrange2-hCM1

    Plasmid

    #196863

    Purpose

    Expression of neural precursor cell expressed developmentally down-regulated 1 (Nedd1) fused to mOrange2 and human (h) Centrosomin motif 1 (CM1). Nedd1 targets CM1 to the centrosome to activate gamma-TuRC

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    Nedd1-mOrange2-hCM1 (Nedd1 Human, Rat)
    

    Expression

    Mammalian

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pelB-MBP-P66-ss

    Plasmid

    #137045

    Purpose

    E. coli expression clone (T7lac promoter) for mature P66 (aa 22-618) from B. burgdorferi with N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66949.1 (p66 Borrelia burgdorferi B31)
    

    Tags

    PelB signal peptide + His10 + maltose binding pro…

    Expression

    Bacterial

    Mutation

    mature P66: lacks the P66 signal peptide

    Promoter

    T7lac

    Available Since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. cryS96

    Plasmid

    #67519

    Purpose

    αB-crystallin minipeptide fused to ELP (S96).

    Depositor

    Andrew MacKay

    Article

    Wang et al J Control Release. 2014 Oct 10;191:4-14.

    Insert

    mini-αB-crystallin fused to ELP S96 (VPGSG) (CRYAB Human, Synthetic)
    

    Tags

    elastin-like polypeptide (ELP) S96

    Expression

    Bacterial

    Mutation

    mini-αB-crystallin contains residues 73–92 of aB-…

    Promoter

    T7

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

19. Ikaros-CRISPR #1 (PX458-EF1a-pSpCas9(BB)-2A-GFP)

    Plasmid

    #75240

    Purpose

    CRISPR/Cas9 plasmid against human Ikaros

    Depositor

    Ria Baumgrass

    Article

    Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

    Insert

    sgRNA against human Ikaros (IKZF1 Human)
    

    Use

    CRISPR

    Available Since

    June 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pNTKV- Elf5-KI-3'UTR-2A-EYFP-NLS

    Plasmid

    #128833

    Purpose

    Knock-in of nuclear EYFP into mouse Elf5 locus

    Depositor

    Yossi Buganim

    Article

    Benchetrit et al Cell Stem Cell. 2019 Jun 6;24(6):983-994

    Insert

    Nuclear EYFP into Elf5 locus (Elf5 Mouse)
    

    Use

    Mouse Targeting

    Available Since

    Aug. 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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