We narrowed to 10,347 results for: plasmids 101

1. pAAV-EF1a-fDIO-Cre

   Plasmid

   #121675

   Purpose

   Expresses Cre recombinase and HA tag in a Flp dependent fashion (fDIO)

   Depositor

   Esteban Engel, Alexander Nectow

   Article

   Schneeberger et al Cell. 2019 Jun 24. pii: S0092-8674(19)30

   Has Service

   AAV Retrograde, AAV1, AAV5, AAV8, and AAV9

   Insert

   NLS-CRE-HA
   

   Use

   AAV

   Tags

   3xHA and SV40-NLS

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Feb. 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBZCas13b

   Plasmid

   #89898

   Purpose

   Bacterial expression for bzCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pPbcas13b

   Plasmid

   #89906

   Purpose

   Bacterial expression for pbCas13b, driven by the lac promoter, and DR-spacer-DR sequence driven by js23119. New spacer sequences can be cloned in between the DRs by digesting the plasmid with BsaI.

   Depositor

   Feng Zhang

   Article

   Smargon et al Mol Cell. 2017 Feb 16;65(4):618-630.e7.

   Insert

   Cas13b
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Lac

   Available Since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV-CaMKII-PinkyCaMP

   Plasmid

   #232858

   Purpose

   An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CaMKII promoter

   Depositor

   Olivia Masseck

   Article

   Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

   Insert

   PinkyCaMP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CaMKII

   Available Since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSLQ5428_pHR_EF1a-mCherry-P2A-Rfx_Cas13d-2xNLS-3xFLAG

   Plasmid

   #155305

   Purpose

   Lentiviral vector encoding Rfx Cas13d fused with 2xNLS, 3xFLAG, and 2A-tagged mCherry.

   Depositor

   Stanley Qi

   Article

   Abbott et al Cell. 2020 May 14;181(4):865-876.e12. do

   Insert

   mCherry 2A-tagged to Ruminococcus flavefaciens XPD3002 Cas13d
   

   Use

   Lentiviral

   Tags

   2xNLS-3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. AAVS1-A2UCOE-EF1a-HSV TK-P2A-H2-Kb SCT-T2A-mCD47-E2A-mCD55-F2A-neo-AAVS1

   Plasmid

   #205446

   Purpose

   human AAVS1-targeting plasmid for expression of HSV TK, H2-Kb SCT, mouse CD47, and mouse CD55

   Depositor

   Deepta Bhattacharya

   Article

   Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

   Insert

   HSV TK-H2-Kb SCT-mCD47-mCD55
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pOTTC476 - pAAV c-fos eYFP

   Plasmid

   #47907

   Purpose

   An AAV vector that expresses eYFP under the c-fos promoter.

   Depositor

   Brandon Harvey

   Article

   Richie et al J Neurosci Methods. 2017 Jun 1;284:1-14.

   Insert

   enhanced Yellow Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   c-fos

   Available Since

   Nov. 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pLIX403_deltaUBA_UBAP2L_mCherry

   Plasmid

   #105287

   Purpose

   puro-selectable lentivirus vector for inducible expression of a UBAP2L-mCherry fusion protein lacking the UBA domain of UBAP2L

   Depositor

   Eugene Yeo

   Article

   Markmiller et al Cell. 2018 Jan 25;172(3):590-604.e13. do

   Insert

   UBAP2L (UBAP2L Human)
   

   Use

   Lentiviral

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   deleted amino acids 20-108

   Promoter

   TRE promoter, Tet ON

   Available Since

   Sept. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pB-TopFlash-NanoLucP

   Plasmid

   #204347

   Purpose

   TopFlash reporter

   Depositor

   Li-Fang Chu

   Article

   Conrad et al Stem Cell Reports. 2023 Dec 12;18(12):23

   Insert

   TopFlash-NanoLucP
   

   Use

   Luciferase

   Tags

   NanoLucP

   Expression

   Mutation

   Promoter

   miniP

   Available Since

   Jan. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. R619-M66-303: CMV51p> HCoV-OC43 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166015

    Purpose

    mammalian expression of HCoV-OC43 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-OC43 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. R619-M91-303: CMV51p> HCoV-NL63 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166017

    Purpose

    mammalian expression of HCoV-NL63 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-NL63 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. R619-M90-303: CMV51p> HCoV-229E S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166016

    Purpose

    mammalian expression of HCoV-229E soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-229E S (spike) (S )
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pZac2.1-gfaABC1D-PinkyCaMP

    Plasmid

    #232859

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under gfaABC1D promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    gfaABC1D

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-CAG-PinkyCaMP

    Plasmid

    #232860

    Purpose

    An AAV vector expressing the mScarlet based calcium indicator PinkyCaMP under CAG promoter

    Depositor

    Olivia Masseck

    Article

    Fink et al bioRxiv [Preprint]. 2025 Jan 11:2024.12.

    Insert

    PinkyCaMP
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available Since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. R619-M89-303: CMV51p> HCoV-HKU1 S-2P-T4f-3C-His8-Strep2x2

    Plasmid

    #166014

    Purpose

    mammalian expression of HCoV-HKU1 soluble spike trimer protein

    Depositor

    Dominic Esposito

    Article

    Esposito et al Protein Expr Purif. 2020 Jun 4;174:10568

    Insert

    HCoV-HKU1 S (spike)
    

    Use

    Tags

    3C-His8-Strep2x2

    Expression

    Mammalian

    Mutation

    Promoter

    CMV51

    Available Since

    March 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pAAV-TPH2-Cre

    Plasmid

    #189616

    Purpose

    Expresses Cre recombinase under the control of the tryptophan hydroxylase promoter

    Depositor

    David Clapham

    Article

    Sheu et al Cell. 2022 Sep 1;185(18):3390-3407.e18.

    Insert

    Cre Recombinase
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Tph2

    Available Since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. APOBEC3G_pLX307

    Plasmid

    #98345

    Purpose

    Lentiviral expression of APOBEC3G

    Depositor

    William Hahn, Sefi Rosenbluh

    Article

    Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

    Insert

    APOBEC3G (APOBEC3G Human)
    

    Use

    Lentiviral

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    E1Fa

    Available Since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. GG-EBNA-MIR302-7guides-PGK-Puro

    Plasmid

    #201960

    Purpose

    EBNA episome plasmid for U6 promoter-driven expression of 7 gRNAs targeting miRNA302/367. Includes PGK-puro selection cassette

    Depositor

    Timo Otonkoski

    Article

    Sokka et al Stem Cell Reports. 2022 Feb 8;17(2):413-

    Insert

    MIR302-7g-PGK-Puro
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available Since

    June 6, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pOTTC946 - pAAV CMV-IE NK1R-IRES-EGFP

    Plasmid

    #102935

    Purpose

    An AAV vector that expresses rat NK1R-IRES-EGFP under the CMV-IE promoter

    Depositor

    Brandon Harvey

    Article

    Nelson et al Neuroscience. 2019 Jun 23;413:77-85. doi

    Insert

    NK1R (Tacr1 Rat)
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV-IE

    Available Since

    Sept. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHAGE2-EF1a-E1MLS-2xMyc-Ku-IRES-Hygro

    Plasmid

    #234950

    Purpose

    Human codon optimized Ku (Mycobacterium) with N-terminal E1 MLS expressing plasmid

    Depositor

    Agnel Sfeir

    Article

    Fu et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

    Insert

    human codon optimized Ku with N-terminal mitochondrial localization signal of pyruvate dehydrogenase E1 subunit
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge