We narrowed to 30,097 results for: LIS;

1. pLVX-Flag-CDK19-WT-IRES-ZsGreen

   Plasmid

   #68858

   Purpose

   Expresses Human CDK19 Wild-Type

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. LLP252 pEF1a-NLS-scFvGCN4-DNMT3a

   Plasmid

   #100941

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic domain (DNMT3A Synthetic, Human)
   

   Use

   Tags

   1xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. LLP185 pLVP-dCas9-DNMT3a V2

   Plasmid

   #100936

   Purpose

   dCas9 and DNMT WT on C terminus, 4 NLS, driven by pGK promoter, with P2A site and PURO gene, within a lentiviral transfer backbone

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pRRL-MND-hCard11 WT-T2A-GFP

   Plasmid

   #163787

   Purpose

   Expression of Wild-type CARD11

   Depositor

   Richard James

   Article

   Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

   Insert

   CARD11 (CARD11 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   MND

   Available since

   Feb. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLVX-Flag-CDK8-W105M-IRES-mCherry

   Plasmid

   #68857

   Purpose

   Expresses Human CDK8 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 8 (CDK8 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pLVX-Flag-CDK19-W105M-IRES-ZsGreen

   Plasmid

   #68859

   Purpose

   Expresses Human CDK19 W105M Mutant

   Depositor

   Matthew Shair

   Article

   Pelish et al Nature. 2015 Sep 28. doi: 10.1038/nature

   Insert

   Cyclin-Dependent Kinase 19 (CDK19 Human)
   

   Use

   Lentiviral

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   W105M

   Promoter

   EF1α

   Available since

   March 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pMXs-GNL

   Plasmid

   #188232

   Purpose

   Polycistronic human Glis1, LIN28 and NANOG were cloned into the pMXs vector

   Depositor

   Young Tang

   Article

   Wang et al Biol Open. 2019 Dec 5;8(12). pii: 8/12/b

   Insert

   Glis1, NANOG and LIN28A separated by 2A sequence (GLIS1, NANOG, LIN28A Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. LLP340 pEF1a-NLS-scFvGCN4-DNMT3a (mutant)

   Plasmid

   #100944

   Purpose

   Single chain variable fragment antibody GCN4 fused to DNMT3a catalytic mutant, binds SunTag, with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, DNMT3a catalytic mutant (DNMT3A Synthetic, Human)
   

   Use

   Tags

   3xTy1

   Expression

   Mammalian

   Mutation

   F636A, E660A, E725A, R295A

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. LLP249 pGK-dCas9-DNMT3a V3

   Plasmid

   #100938

   Purpose

   dCas9 and DNMT WT driven by pGK promoter and Puromycin driven by EF1a

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   dCas9, DNMT3a catalytic domain (DNMT3A Human, S.pyogenes)
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and 3xTy1

   Expression

   Mammalian

   Mutation

   D10A, H840A for dCas9

   Promoter

   Available since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pRRL-MND-hCard11 R72L-T2A-GFP

    Plasmid

    #163784

    Purpose

    Expression of R72L CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R72L

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pRRL-MND-hCard11 R30W-T2A-GFP

    Plasmid

    #163786

    Purpose

    Expression of R30W CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R30W

    Promoter

    MND

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pRRL-MND-hCard11 K41R-T2A-GFP

    Plasmid

    #163779

    Purpose

    Expression of K41R CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K41R

    Promoter

    MND

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pRRL-MND-hCard11 R35Q-T2A-GFP

    Plasmid

    #163774

    Purpose

    Expression of R35Q CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R35Q

    Promoter

    MND

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pRRL-MND-hCard11 delta e4-T2A-GFP

    Plasmid

    #163771

    Purpose

    Expression of CARD domain variant with a deletion of exon 4

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 4

    Promoter

    MND

    Available since

    Feb. 3, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRRL-MND-hCard11 R72Q-T2A-GFP

    Plasmid

    #163783

    Purpose

    Expression of R72Q CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R72Q

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pRRL-MND-hCard11 D58G-T2A-GFP

    Plasmid

    #163773

    Purpose

    Expression of D58G CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    D58G

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pRRL-MND-hCard11 delta e3-4-T2A-GFP

    Plasmid

    #163772

    Purpose

    Expression of CARD domain variant with a deletion of exons 3 and 4.

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3-4

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pRRL-MND-hCard11 delta e3-T2A-GFP

    Plasmid

    #163770

    Purpose

    Expression of CARD domain variant with a deletion of exon 3

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Del exon 3

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRRL-MND-hCard11 K69L-T2A-GFP

    Plasmid

    #163781

    Purpose

    Expression of K69L CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K69L

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRRL-MND-hCard11 R35K-T2A-GFP

    Plasmid

    #163776

    Purpose

    Expression of R35K CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R35K

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pRRL-MND-hCard11 K41Q-T2A-GFP

    Plasmid

    #163777

    Purpose

    Expression of K41Q CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K41Q

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRRL-MND-hCard11 K41L-T2A-GFP

    Plasmid

    #163778

    Purpose

    Expression of K41L CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K41L

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRRL-MND-hCard11 K69Q-T2A-GFP

    Plasmid

    #163780

    Purpose

    Expression of K69Q CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K69Q

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRRL-MND-hCard11 R72K-T2A-GFP

    Plasmid

    #163785

    Purpose

    Expression of R72K CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R72K

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pRRL-MND-hCard11 K69R-T2A-GFP

    Plasmid

    #163782

    Purpose

    Expression of K69R CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    K69R

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRRL-MND-hCard11 R35L-T2A-GFP

    Plasmid

    #163775

    Purpose

    Expression of R35L CARD domain variant

    Depositor

    Richard James

    Article

    Meitlis et al Am J Hum Genet. 2020 Nov 12. pii: S0002-

    Insert

    CARD11 (CARD11 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    R35L

    Promoter

    MND

    Available since

    Feb. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. TRUPATH Triple (bidirectional) GasS

    Plasmid

    #196055

    Purpose

    Encodes a G alpha subunit (GNAS2) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphasS-RLuc8
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. TRUPATH Triple Gai1

    Plasmid

    #196048

    Purpose

    Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

    Depositor

    Justin English

    Article

    Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

    Inserts

    GAlphai1-RLuc8 (GNAI1 Human)
    GGamma9-GFP2
    GBeta3 (GNB3 Human)
    

    Use

    Luciferase

    Tags

    GFP2 and GSAG linker

    Expression

    Mammalian

    Mutation

    RLuc8 and flanking SGGGGS linkers have been inser…

    Promoter

    CMV

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pENTR D-TOPO-C3_33

    Plasmid

    #60275

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, which can be shuttled into a Gateway destination vector, such as pGL4.23-Gateway.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    GLIS3 enhancer (GLIS3 Human)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pGL4.23 C3_33

    Plasmid

    #60312

    Purpose

    This plasmid contains a human pancreatic islet active enhancer, a minimal promoter and a luc2 gene.

    Depositor

    Jorge Ferrer

    Article

    Pasquali et al Nat Genet. 2014 Feb;46(2):136-43. doi: 1

    Insert

    GLIS3 enhancer (GLIS3 Human)
    

    Use

    Luciferase

    Tags

    Luciferase

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2014

    Availability

    Academic Institutions and Nonprofits only