We narrowed to 8,596 results for: Set

1. qTAG-C-mStayGold-Puro-H2BC11

   Plasmid

   #227332

   Purpose

   Donor template for mStayGold-2A-Puro insertion into the C-terminus of the H2BC11 locus. For nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 (Addgene #207755)

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Homology Arms flanking a mStayGold-2A-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Promoter

   Promoterless

   Available Since

   Nov. 12, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEN84 - CTCF-AID[71-114]-eGFP-FRT-Puro-FRT targeting construct

   Plasmid

   #86230

   Purpose

   Targeting vector to introduce an AID-eGFP cassette at the mouse CTCF locus using puromycine selection. Auxin-inducible degron system.

   Depositor

   Benoit Bruneau

   Article

   Nora et al Cell. 2017 May 18;169(5):930-944.e22. do

   Insert

   AID[71-114]-eGFP
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   May 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. MF83_PB-TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA

   Plasmid

   #176627

   Purpose

   TF B self-activation cassette in MultiFate-2.2 and MultiFate-3

   Depositor

   Michael Elowitz

   Article

   Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

   Insert

   TRE3G-6x42bs-10x(37bs_37bs)-miniCMV-NLS-FKBP12F36V-37ZFR2AR11AR39AR67A-VP16-NLS-DHFR-IRES-mCitrine-PEST-BGHpA
   

   Use

   Synthetic Biology

   Available Since

   Jan. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTK Cas9-2A-Citrine

   Plasmid

   #92393

   Purpose

   Enhancer/reporter plasmid for tissue-specific expression of Cas9 with 2A-Citrine reporter. Contains BsmBI-flanked LacZ cloning cassette for rapid GoldenGate-based cloning of specific enhancers.

   Depositor

   Tatjana Sauka-Spengler

   Article

   Williams et al Development. 2018 Feb 23;145(4). pii: de

   Insert

   Cas9 2A Citrine
   

   Use

   CRISPR

   Expression

   Mammalian

   Promoter

   thymidine kinase promoter

   Available Since

   Aug. 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pG2J

   Plasmid

   #102882

   Purpose

   Expresses OsTIR1 via the ADH1 promoter, and the mGFP-AID via the TEF1 promoter in yeast. Both cassettes are integrated in HO locus. Assembly and characterization of the auxin-inducible degradation.

   Depositor

   Matthias Heinemann

   Article

   Papagiannakis et al Mol Cell. 2017 Jan 19;65(2):285-295. doi

   Inserts

   OsTIR1
   mGFP-AID
   

   Tags

   AID(71-114)

   Expression

   Yeast

   Promoter

   ADH1 and TEF1

   Available Since

   Jan. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR-eGFP-attP(bxb)-*BsdR

   Plasmid

   #183751

   Purpose

   Bxb1 landing pad cassette.

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al Cell Rep Methods. 2022 Sep 22;2(10):1003

   Insert

   loxP-eGFP-attP-Bsd-lox251
   

   Use

   Cre/Lox and Synthetic Biology

   Expression

   Mammalian

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR-PGK-scFv GCN4-sfGFP-VP64

   Plasmid

   #122133

   Purpose

   Express a scFv which binds to GCN4, sfGFP and VP64 cassette from the PGK promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   scFv GCN4-sfGFP-VP64
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   PGK

   Available Since

   May 13, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDFDuet-nsp7-nsp8

   Plasmid

   #159092

   Purpose

   Coexpression construct of Nsp7 with an N-terminal His-tag and Nsp8

   Depositor

   Seth Darst

   Article

   Chen et al Cell. 2020 Sep 17;182(6):1560-1573.e13.

   Inserts

   NSP7
   NSP8
   

   Tags

   His

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pWZ403

   Plasmid

   #208113

   Purpose

   mSwAP-In marker cassette 1 with mScarlet-PuroTK

   Depositor

   Jef Boeke

   Article

   Zhang et al Nature. 2023 Nov 1. doi: 10.1038/s41586-

   Insert

   mScarlet-PuroTK
   

   Tags

   P2A

   Expression

   Bacterial and Mammalian

   Promoter

   EF1alpha

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. qTAG-C-moxGFP-Puro-H2BC11

    Plasmid

    #207758

    Purpose

    Donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 17, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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11. UAS-FRT-GFP-FRT-u(M)Cas9

    Plasmid

    #127388

    Purpose

    Cas9 inducible by removal of a GFP flip-out cassette

    Depositor

    Michael Boutros

    Article

    Port et al Elife. 2020 Feb 13;9. pii: 53865. doi: 1

    Insert

    FRT-GFP-FRT-uORF(M)Cas9
    

    Use

    CRISPR

    Expression

    Insect

    Available Since

    June 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.minBG-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231365

    Purpose

    minBG-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAAV.Ple155-CI-EGFP-W3SL_2x-U6-sasgAi14

    Plasmid

    #231366

    Purpose

    Ple155-driven EGFP, also encoding U6-driven sgRNAs to direct saCas9 to both sides of stop cassette in Ai14 mice.

    Depositor

    Viviana Gradinaru

    Article

    Coughlin et al Nat Biotechnol. 2025 Mar 20. doi: 10.103

    Insert

    EGFP
    

    Use

    AAV

    Available Since

    March 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

    Plasmid

    #140018

    Purpose

    Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

    Depositor

    Simon Elsaesser

    Article

    Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

    Insert

    EcoTyrRS
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Y37L, D182S, F183M, L186A

    Promoter

    EF1

    Available Since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLV.5’VIM-eGFP-KI

    Plasmid

    #170547

    Purpose

    Expresses a sgRNA for 5' tagging to VIM and contains a cassette with eGFP flanked by homology arms for VIM

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Inserts

    Left Homology arm for VIM knockin
    Right Homology arm for VIM knockin
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. qTAG-C-miRFP670nano3-Blast-H2BC11

    Plasmid

    #207762

    Purpose

    Donor template for miRFP670nano3-2A-Blast insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    H2BC11 Homology Arms flanking a miRFP670nano3-Blast Cassette (H2BC11 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Available Since

    Nov. 29, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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17. pAAV-U6-DR(RfxCas13d)-CAG-CasRx-pa

    Plasmid

    #233035

    Purpose

    To express a RfxCas13d-compatible gRNA and to express HA Tagged CasRx from a CAG promoter

    Depositor

    Jonathan Ploski

    Article

    Back et al Mol Ther Methods Clin Dev. 2025 Aug 20;3

    Insert

    RfxCas13d-compatible gRNA expression cassette and CasRx
    

    Use

    AAV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAGEN-WPRE-bGH-DEST (JDW 1309)

    Plasmid

    #229815

    Purpose

    A CAGGS driven, gateway compatible destination vector that contains a WPRE downstream of the destination cassette to stabilize transcripts

    Depositor

    Joshua Wythe

    Article

    Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Feb. 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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19. MF82_PB-TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA

    Plasmid

    #176626

    Purpose

    TF A self-activation cassette in MultiFate-2.2 and MultiFate-3

    Depositor

    Michael Elowitz

    Article

    Zhu et al Science. 2022 Jan 21;375(6578):eabg9765.

    Insert

    TRE3G-12x42bs-10x(BCRbs_BCRbs)-miniCMV-NLS-FKBP12F36V-BCRZFR39A-VP16-NLS-DHFR-IRES-mCherry-PEST-BGHpA
    

    Use

    Synthetic Biology

    Available Since

    Jan. 27, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. qTAG-AAVS1-PGK-Puro-moxGFP

    Plasmid

    #227275

    Purpose

    AAVS1 targeting donor for the insertion of Puro and a medium strength PGK expressing moxGFP. To be co-transfected with sgRNA plasmid px330-AAVS1 (Addgene #227272)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    AAVS1 Homology Arms flanking a 2A-Puro-PGK-moxGFP cassette (AAVS1 Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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