We narrowed to 8,419 results for: BLI

1. plentiCRISPRv2-PURO-LYSET-gRNA1

   Plasmid

   #210489

   Purpose

   CRISPR pooled KO of human LYSET

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-gRNA1 (LYSET Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. plentiCRISPRv2-PURO-LYSET-gRNA2

   Plasmid

   #210490

   Purpose

   CRISPR pooled KO of human LYSET

   Depositor

   Jan Carette

   Article

   Richards et al Science. 2022 Sep 8:eabn5648. doi: 10.11

   Insert

   LYSET-gRNA2 (LYSET Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti-H3.3(WT)-3AID-HA-2A-mCherryBSD

   Plasmid

   #225697

   Purpose

   Lentiviral expression of AID degron tagged Wildtype H3.3 and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (WT) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLenti-H3.3(K27M)-3AID-HA-2A-mCherryBSD

   Plasmid

   #225698

   Purpose

   Lentiviral expression of AID degron tagged H3.3(K27M) and mCherry fused BlasticidinR

   Depositor

   Lacra Bintu

   Article

   Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

   Insert

   H3.3 (K27M) (H3-3A, H3-3B Human)
   

   Use

   Lentiviral and Synthetic Biology

   Tags

   3xAID HA and T2A-mCherry

   Expression

   Mammalian

   Mutation

   K27M

   Promoter

   pEF1A

   Available since

   Oct. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. SspB-HaloTag-DHPH-ITSN1

   Plasmid

   #176113

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDONR223-H-RAS V12

   Plasmid

   #31201

   Depositor

   David Root

   Article

   Yang et al Nat Methods. 2011 Jun 26;8(8):659-61. do

   Insert

   H-Ras (HRAS Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   G12V constitutively active

   Promoter

   Available since

   July 12, 2011

   Availability

   Academic Institutions and Nonprofits only

7. SspB-iRFP-DHPH-ITSN1

   Plasmid

   #176117

   Purpose

   Heterodimerization with iLID, Cdc42 activation

   Depositor

   Dorus Gadella

   Article

   Mahlandt et al Elife. 2023 Jul 14;12:RP84364. doi: 10.7

   Insert

   ITSN1 (ITSN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-pSMANTIS_1kb_KLF2mut 449,548,732,899,884 (All)

   Plasmid

   #194190

   Purpose

   Includes the promoter (1kb) of SMTS with 5 mutated KLF binding sites (positions 449,548,732,899,884)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   5 mutated KLF binding sites (positions 449,548,73…

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pGL3-pSMANTIS_1kb_KLF2mut 899

   Plasmid

   #194193

   Purpose

   Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 899)

   Depositor

   Ralf Brandes, Matthias Leisegang

   Article

   Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

   Insert

   SMANTIS promoter (1kb) (SMANTIS Human)
   

   Use

   Luciferase

   Tags

   Expression

   Mutation

   mutated KLF binding sites (position 899)

   Promoter

   SMANTIS promoter (1kb), mutated

   Available since

   Jan. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTO_Snf7_LAP

    Plasmid

    #101851

    Purpose

    Tet-inducible expression of Snf7 from S. cerevisiae with a C-terminal GFP and long linker (LAP tag), compatible with Flp-In recombination for stable integration

    Depositor

    Daniel Gerlich

    Article

    Mierzwa et al Nat Cell Biol. 2017 Jul;19(7):787-798. d

    Insert

    SNF7 (SNF7 Budding Yeast)
    

    Use

    Gateway cloning, flp-frt recombination, tet-induc…

    Tags

    Localization and Affinity Purification (LAP) tag

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Dec. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLV-SFFV-CKAP4(2xlumen)-WPRE-UbC-Emerald

    Plasmid

    #225956

    Purpose

    Lentiviral vector plasmid expressing human CKAP4 mutant with an additional luminal domain under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    CKAP4 (CKAP4 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Full-length CKAP4 with additional luminal domain …

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLV-SFFV-myc-STIM1-WPRE-UbC-Emerald

    Plasmid

    #225939

    Purpose

    Lentiviral vector plasmid expressing human stromal interaction molecule 1 (STIM1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    STIM1 (STIM1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLV-SFFV-myc-ATL1-WPRE-UbC-Emerald

    Plasmid

    #225944

    Purpose

    Lentiviral vector plasmid expressing human atlastin GTPase 1 (ATL1) fused to myc-tag under the spleen focus-forming virus (SFFV) promoter and Emerald under the Ubiquitin C (UbC) promoter

    Depositor

    Evan Reid, Richard Eva, Bart Nieuwenhuis

    Inserts

    ATL1 (ATL1 Human)
    Emerald
    

    Use

    Lentiviral

    Tags

    myc-tag

    Expression

    Mammalian

    Mutation

    Promoter

    SFFV and UbC

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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14. AID-C12*-n'Cas9-BE4max

    Plasmid

    #216730

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and n'Cas9 (Cas9 with H840A mutation) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-n'Cas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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15. pSCBE3-NG-HF1

    Plasmid

    #218160

    Purpose

    This plasmid harbors the base editor SCBE3-NG-HF1 along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-HF1
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N497A…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSCBE3-NG-Hypa

    Plasmid

    #218161

    Purpose

    This plasmid harbors the base editor SCBE3-NG-Hypa along with an sgRNA cloning cassette, facilitating high-fidelity cytosine base editing at targets bearing NG PAM in Streptomyces.

    Depositor

    Yuhui Sun

    Article

    Wang et al Nat Commun. 2024 Jul 7;15(1):5687. doi:

    Inserts

    scbe3-NG-Hypa
    a programmable sgRNA cloning cassette
    

    Use

    CRISPR; Sgrna transcription

    Tags

    Expression

    Bacterial

    Mutation

    Mutations in the portion of SpCas9 : D10A / N692A…

    Promoter

    rpsL promoter

    Available since

    May 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. AID-C12*-dCas9-BE4max

    Plasmid

    #216731

    Purpose

    Expresses a BE4max base editing construct comprised of the hyperactive AID-C12* and dCas9 (Cas9 with D10A and H840A mutations) in mammalian cells.

    Depositor

    Rahul Kohli

    Article

    Berrios et al Nucleic Acids Res. 2024 Jan 23:gkae024.

    Insert

    AID-C12*-dCas9-BE4max (AICDA Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. An R57A, R59A CAAX pMT2

    Plasmid

    #206119

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Nov. 28, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. An R57A, R59A HA CAAX pMT2

    Plasmid

    #206126

    Purpose

    Cav2.1 N-terminus (amino acids 1-100) with R57A and R59A mutations that prevent dominant-negative suppression of Cav2.1 currents and a C-terminal CAAX (last 10 aa of H.Ras) motif and a HA tag

    Depositor

    Annette Dolphin

    Article

    Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

    Insert

    cacna1a (Cacna1a Rat)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    R57A, R59A

    Promoter

    Ad MLP/TPL/SV40

    Available since

    Oct. 31, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pGL3-pSMANTIS_1kb_KLF2mut 732

    Plasmid

    #194195

    Purpose

    Includes the promoter (1kb) of SMTS with mutated KLF binding sites (position 732)

    Depositor

    Ralf Brandes, Matthias Leisegang

    Article

    Leisegang et al Eur Heart J. 2019 Aug 7;40(30):2523-2533

    Insert

    SMANTIS promoter (1kb) (SMANTIS Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    mutated KLF binding sites (position 732)

    Promoter

    SMANTIS promoter (1kb), mutated

    Available since

    Jan. 24, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge