177,602 results
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Plasmid#123285PurposeLocalization: Endoplasmic ReticulumDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertSec61 (SEC61B Human)
TagsHaloTag7ExpressionMammalianPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV SYN flex PSAM4 GlyR IRES EGFP
Plasmid#119741PurposeCre-dependent chemogenetic inhibitor expressionDepositorHas serviceAAV5 and AAV9InsertPSAM4 GlyR IRES eGFP (CHRNA7 Human, Synthetic)
UseAAVExpressionMammalianMutationL131G, Q139L, Y217FPromotersynapsinAvailable sinceMarch 18, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHAGE-BRAF
Plasmid#116719PurposeLentiviral expression of BRAFDepositorAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGEX6P1-DEST
Plasmid#119749PurposeAllows Gateway cloning of gene of interest into bacterial expression vector with PreScission Protease cleavable N-terminal GST tag.DepositorTypeEmpty backboneTagsGSTExpressionBacterialPromotertac promoterAvailable sinceJan. 29, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCT5-bac1.8
Plasmid#119871PurposeConstitutive expression of sfGFP gene in Bacillus subtilis, B. megaterium, and Escherichia coliDepositorInsertsCymR'
sfGFP
UseSynthetic BiologyExpressionBacterialMutationSNP in 592 bpPromoterPserA promoter and Pveg promoter with CuO sequenceAvailable sinceJan. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLNCX2-mCherry-VPS4A
Plasmid#115334Purposeexpresses mCherry-VPS4A in mammalian cellsDepositorInsertVPS4A (VPS4A Human)
UseRetroviralTagsmCherryExpressionMammalianMutationsilent mutation in position 4050 C to TPromoterCMVAvailable sinceSept. 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLHCX-ATF4 mScarlet NLS
Plasmid#115970PurposeER-stress sensor - ATF4 translation at ORF3 fluorescent reporter with mScarlet-I (retroviral vector backbone)DepositorInsertactivating transcription factor 4 (ATF4 Human, Synthetic)
UseRetroviralTagsHA tag, c-myc NLS, and mScarlet-IExpressionMammalianPromoterCMVAvailable sinceSept. 25, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pg-HIF-1alpha-EGFP
Plasmid#87204PurposeC-terminally EGFP-tagged human HIF-1aDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHIF-1a (HIF1A Human)
TagsEGFPExpressionMammalianMutationL40F, M88V, Q814RPromoterCMVAvailable sinceSept. 4, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEGFP‐RNASEH2A
Plasmid#108700PurposeFor expression of N-terminally eGFP-tagged human RNASEH2A in mammalian cellsDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRNASEH2A (RNASEH2A Human)
TagsGFPExpressionMammalianPromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGP-AAV-syn-FLEX-jGCaMP7f-WPRE
Plasmid#104492PurposeAAV-mediated expression of ultrasensitive protein calcium sensor under the Syn promoter, Cre mediated flip-excision switchDepositorHas serviceAAV1, AAV9, AAV PHP.eB, and AAV RetrogradeInsertjGCaMP7f
UseAAV and Cre/LoxTagsT7 epitope, Xpress tag, 6xHisExpressionMammalianPromoterSynapsinAvailable sinceApril 20, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA
Plasmid#107270PurposeExpresses Cas9 upon induction with doxicycline.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertshSpCas9
rtTA
bGH polyA-SV40polyA
Tags3xFlag, Nucleoplasmin NLS, and SV40 NLSExpressionMammalianPromoterCAG and TREAvailable sinceApril 19, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pET28a-MH6-RspCas13d
Plasmid#108305PurposeExpresses E. coli codon optimized RspCas13d in the pET28a backbone.DepositorInsertRspCas13d
UseCRISPRTagsmH6 tagExpressionBacterialPromoterlacAvailable sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pBb(RSF1010)1k-GFPuv
Plasmid#106395PurposeIPTG-inducible trc promoter expressing gfpuv on RSF1010 originDepositorInsertGFPuv
ExpressionBacterialPromotertrcAvailable sinceMarch 28, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-Puro_siKD
Plasmid#86695PurposeTetracycline inducible expression of shRNA targeted to AAVS1 locusDepositorTypeEmpty backboneExpressionMammalianPromoterH1 TOAvailable sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mScarlet-I-LaminB
Plasmid#98831PurposeIn vivo visualization of the nuclear envelope (can be used for colocalization studies)DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertCytERM
TagsmScarlet-IExpressionMammalianMutation*(see below)PromoterCMVAvailable sinceNov. 14, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pHR_Gal4UAS_Tbet_T2A_GFP_PGK_mCherry
Plasmid#85431PurposeLentiviral vector for Gal4 inducible Tbet T2A GFP and constitutive mCherry expressionDepositorInserthuman Tbet
UseLentiviralAvailable sinceOct. 6, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcrDNA3.1-PRKACA
Plasmid#100890PurposeMammalian expression of PRKACADepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertProtein Kinase CAMP-Activated Catalytic Subunit Alpha (PRKACA Human)
ExpressionMammalianMutationIsoform 1 wild typePromoterCMVAvailable sinceSept. 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
HA-human-BNIP3
Plasmid#100781PurposeMammalian expression of BNIP3DepositorAvailable sinceSept. 11, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA4 HisMax-V5-GFP-RRBP1
Plasmid#92150PurposeExpresses V5- and EGFP-tagged human RRBP1 (with 54 decapeptide repeats) in mammalian cells.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertRRBP1 (RRBP1 Human)
TagsCalreticulin signal peptide, EGFP (A207K mutation…ExpressionMammalianMutationIn RRBP1: N951S and T1329L. E1279 is also missing.PromoterCMVAvailable sinceAug. 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AbVec2.0-IGHG4
Plasmid#99578PurposeExpression of secretory immunoglobulin heavy chains in mammalian cells, human IgG4 isotypeDepositorTypeEmpty backboneExpressionMammalianPromoterhCMV IE1 gene promoterAvailable sinceAug. 29, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by