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MTK4b_004

Plasmid

#123843

Purpose

Encodes spacer sequence as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Spacer

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Blast-cfCdc42_2

Plasmid

#26703

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

Cdc42

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

RNAi to canine Cdc42

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
pRSET FLIPE-100u

Plasmid

#13539

Depositor

Wolf Frommer

Article

Okumoto et al Proc Natl Acad Sci U S A. 2005 Jun 14. 1

Insert

FLIP-E S118W

Tags

ECFP and Venus

Expression

Bacterial

Mutation

S118W in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pLKO.1-puro-cfRab8a_2

Plasmid

#26707

Depositor

Keith Mostov

Article

Bryant et al Nat Cell Biol. 2010 Nov;12(11):1035-45.

Insert

dog Rab8a RNAi

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

Dog Rab8a RNAi.

Available Since

Dec. 14, 2010

Availability

Academic Institutions and Nonprofits only
PCBP3_pCSdest

Plasmid

#53957

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PCBP3 (PCBP3 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
MTK3b_020

Plasmid

#123801

Purpose

Encodes synTF ZF binding domain as a Type 3b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SynTF

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV-1-830

Plasmid

#40963

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

supervillin (SVIL Bovine)

Tags

EGFP

Expression

Mammalian

Mutation

amino acids 1-830

Available Since

Dec. 12, 2012

Availability

Academic Institutions and Nonprofits only
MTK2_017

Plasmid

#123709

Purpose

Encodes truncated pCMV as a Type 2 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

pCMV-B

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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knob-s48i48

Plasmid

#68391

Purpose

Knob protein fused to elastin-like polypeptide (S48I48)

Depositor

Andrew MacKay

Article

Sun et al J Control Release. 2011 Oct 30;155(2):21

Insert

Knob-S48I48

Tags

Knob

Expression

Bacterial

Promoter

T7

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available Since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pLU-YFP-ICP0FxE

Plasmid

#134564

Purpose

Expresses YFP-ICP0 fusion protein with a RING finger deletion (FXE)

Depositor

Susan Janicki

Article

Newhart et al J Cell Sci. 2012 Nov 15;125(Pt 22):5489-

Insert

YFP-ICP0

Tags

None

Expression

Mammalian

Mutation

RING finger deletion (FXE)

Available Since

March 11, 2020

Availability

Academic Institutions and Nonprofits only

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PDE9A_pCSdest

Plasmid

#53944

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

PDE9A (PDE9A Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

Sept. 8, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pRSET FLII32Pglu-2.2m

Plasmid

#13566

Depositor

Wolf Frommer

Article

Deuschle et al Protein Sci. 2005 Sep . 14(9):2304-14.

Insert

FLIPglu F16A P32S-ECFP-D33R

Tags

6xHis and Citrine

Expression

Bacterial

Mutation

F16A, ECFP insert in binding protein

Available Since

Dec. 15, 2006

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 5ARC (171–961)

Plasmid

#132638

Purpose

expresses mouse Tankyrase1 5ARC (171–961) in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2018 Jun;27(6):1057-1067. d

Insert

Tankyrase1 (171–961)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pHH0103_EGFR_Recep-L

Plasmid

#109920

Purpose

Protein expression and purification of EGFR_Recep-L

Depositor

Sachdev Sidhu

Article

Teyra et al Mol Syst Biol. 2020 Dec;16(12):e9310. do

Insert

EGFR_Recep-L

Expression

Bacterial

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pANY3

Plasmid

#112197

Purpose

This plasmid is used for efficient cloning of DNA and temperature-induced expression of protein in E. coli.

Depositor

Yingfeng An

Article

Gao et al J Agric Food Chem. 2018 Jun 20;66(24):61

Type

Empty backbone

Tags

His

Expression

Bacterial

Available Since

Aug. 10, 2018

Availability

Academic Institutions and Nonprofits only

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pCAGGS/Daxx myc S502A

Plasmid

#19281

Depositor

Philip Leder

Article

Ecsedy et al Mol Cell Biol 2003 Feb;23(3):950-60.

Insert

Daxx myc (Daxx Mouse)

Expression

Mammalian

Available Since

Sept. 15, 2008

Availability

Academic Institutions and Nonprofits only
EZH2-TALEN_Left_No_FLAG

Plasmid

#79899

Purpose

Untagged EZH2-TALEN_Left to target the N-terminus of human EZH2

Depositor

Yannick Doyon

Article

Dalvai et al Cell Rep. 2015 Oct 7. pii: S2211-1247(15

Insert

EZH2-TALEN_Left_No_FLAG

Use

TALEN

Expression

Mammalian

Available Since

July 29, 2016

Availability

Academic Institutions and Nonprofits only

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MTK4b_002

Plasmid

#123841

Purpose

Encodes SV40 polyadenylation signal as a Type 4b part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

SV40 pA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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MTK4a_005

Plasmid

#123819

Purpose

Encodes Mitochondria Targeting Sequence as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

MTS

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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