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pB-Orthogonal_LTR5Hs_CARGO

Plasmid

#248159

Purpose

Encodes a set of guide RNAs targeting LTR5Hs elements (orthogonal to Addgene #191316)

Depositor

Joanna Wysocka

Article

Fueyo et al Nature. 2025 Oct 1. doi: 10.1038/s41586-

Insert

Array of guide RNAs

Use

CRISPR

Tags

mCherry

Expression

Mammalian

Available Since

Jan. 30, 2026

Availability

Academic Institutions and Nonprofits only

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pACYC-PJ23100-dpGFP

Plasmid

#249257

Purpose

An avGFP mutant designed by DeepDE that achieved a remarkable 74.3-fold increase in activity over four rounds of evolution, far surpassing the benchmark superfolder GFP.

Depositor

Xiaofeng Yang

Article

Li et al iScience. 2025 Aug 7;28(9):113324. doi:

Insert

dpGFP

Expression

Bacterial

Promoter

PJ23100

Available Since

Jan. 28, 2026

Availability

Academic Institutions and Nonprofits only

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mito_mCherry_HA

Plasmid

#244042

Purpose

anchoring mCherry-1x gp41MoonTag to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

CISD1-mCherry-HA

Tags

HA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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H2B_mCherry_SunTag

Plasmid

#244044

Purpose

anchoring mCherry-1x SunTag to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

HIST1H2BJ-mCherry-SunTag

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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H2B_mCherry_HA

Plasmid

#244046

Purpose

anchoring mCherry-1x HA to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

HIST1H2BJ-mCherry-HA

Tags

HA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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SunTag_mCherry_H2B

Plasmid

#244048

Purpose

anchoring 1xSunTag-mCherry to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

SunTag_mCherry_HIST1H2BJ

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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MoonTag_mCherry_H2B

Plasmid

#244049

Purpose

anchoring 1xMoonTag-mCherry to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

MoonTag_mCherry_HIST1H2BJ

Tags

MoonTag (gp41)

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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HA_mCherry_H2B

Plasmid

#244050

Purpose

anchoring 1xHA-mCherry to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

HA_mCherry_HIST1H2BJ

Tags

HA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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ALFA_mCherry_H2B

Plasmid

#244051

Purpose

anchoring 1xALFA-mCherry to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

ALFA_mCherry_HIST1H2BJ

Tags

ALFA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_SunTag_H2B

Plasmid

#244052

Purpose

anchoring mCherry-1x SunTag to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-SunTag-HIST1H2BJ

Tags

mCherry-SunTag

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_MoonTag_H2B

Plasmid

#244053

Purpose

anchoring mCherry-1x MoonTag to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-MoonTag-HIST1H2BJ

Tags

mCherry-MoonTag

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_HA_H2B

Plasmid

#244054

Purpose

anchoring mCherry-1x HA to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-HA-HIST1H2BJ

Tags

mCherry-HA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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mCherry_ALFA_H2B

Plasmid

#244055

Purpose

anchoring mCherry-1x ALFAto the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

mCherry-ALFA-HIST1H2BJ

Tags

mCherry-ALFA

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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Vimentin_mCherry_SunTag

Plasmid

#244056

Purpose

anchoring mCherry-1x SunTag to the intermediate filaments

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

NtVimentin-mCherry-SunTag

Tags

sunTagV4

Expression

Mammalian

Available Since

Nov. 19, 2025

Availability

Academic Institutions and Nonprofits only

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pET29b_cpCys203

Plasmid

#247771

Purpose

bacterial expression of cpCys203 FRET-based cysteine sensor

Depositor

Alan Ashworth

Article

Abrams et al Cell Chem Biol. 2025 Nov 10:S2451-9456(2

Insert

cpCys203 Cysteine Sensor

Tags

ECFP and Venus and His6-TEV

Expression

Bacterial

Available Since

Nov. 18, 2025

Availability

Academic Institutions and Nonprofits only

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anti-gp41_Nb_GFP

Plasmid

#244062

Purpose

fusion of the MoonTag binder (nanobody2H10) to sfGFP

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

anti-gp41_Nb_sfGFP_GB1

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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anti-ALFA_Nb_mEGFP

Plasmid

#244064

Purpose

fusion of the ALFA binder (nanobody) to mEGFP

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

anti-ALFA_Nb_mEGFP

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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Vimentin_mCherry_MoonTag

Plasmid

#244057

Purpose

anchoring mCherry-1x MoonTag to the intermediate filaments

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

NtVimentin-mCherry-MoonTag

Tags

MoonTag (gp41)

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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H2B_mCherry_MoonTag

Plasmid

#244045

Purpose

anchoring mCherry-1x MoonTag to the chromatin/nucleus

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

HIST1H2BJ-mCherry-MoonTag

Tags

MoonTag (gp41)

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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mito_mCherry_SunTag

Plasmid

#244040

Purpose

anchoring mCherry-1x SunTag to the mitochondrial membrane

Depositor

Markus Affolter

Article

Vigano et al Development. 2021 Mar 16;148(6):dev19170

Insert

CISD1-mCherry-SunTag

Tags

sunTagV4

Expression

Mammalian

Available Since

Oct. 29, 2025

Availability

Academic Institutions and Nonprofits only

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