We narrowed to 8,596 results for: Set

1. pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

   Plasmid

   #140205

   Purpose

   Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

   Depositor

   Yit Heng Chooi

   Article

   Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

   Inserts

   mCherry
   sgRNA E1
   

   Use

   CRISPR and Synthetic Biology; Proof-of-concept te…

   Mutation

   G174D

   Promoter

   PelcA and U3

   Available Since

   Sept. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. PV225

   Plasmid

   #132334

   Purpose

   dCas9 plant transcriptional activator (AT-CBF1) linked constitutive expression cassette for Zea mays

   Depositor

   Joshua Young

   Article

   Young et al Commun Biol. 2019 Oct 18;2:383. doi: 10.

   Insert

   dCas9-AT-CBF1 (CBF1 Cas9 (Streptococcus pyogenes); AT-CBF1 (Arabidopsis thaliana))
   

   Tags

   AT-CBF1

   Expression

   Plant

   Mutation

   Codon optimize for expression and stability in Ze…

   Available Since

   Oct. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. sgRNA3_IL1RN

   Plasmid

   #64151

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA3. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA3_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. qTAG-C-moxGFP-Puro-H2BC11-MMEJ

   Plasmid

   #207760

   Purpose

   MMEJ donor template for moxGFP-2A-Puro insertion into the C-terminus of the H2BC11 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H2BC11 Addgene #207755

   Depositor

   Laurence Pelletier

   Article

   Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

   Insert

   H2BC11 Short Homology Arms flanking a moxGFP-Puro Cassette (H2BC11 Human)
   

   Use

   CRISPR; Donor template

   Expression

   Mammalian

   Available Since

   Nov. 15, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pv9_MBD1_MBD-WT-13XL-BASU

   Plasmid

   #179416

   Purpose

   Cell line generation via recombination-mediated cassette exchange (RMCE) and stable expression of MBD1.MBD-WT-13XL-BASU

   Depositor

   Tuncay Baubec

   Article

   Villasenor et al Nat Biotechnol. 2020 Mar 2. pii: 10.1038

   Insert

   MBD1.MBD-WT-13XL-BASU
   

   Expression

   Mammalian

   Promoter

   CAGGS

   Available Since

   Oct. 27, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. Direct-seq_lentiGuide-Puro-tRNA-Tail-8A8G

   Plasmid

   #157984

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tail-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   March 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. Direct-seq_lentiGuide-Puro-tRNA-Tetra Loop-8A8G

   Plasmid

   #157985

   Purpose

   Expresses programmed gRNA scaffold from U6 promoter. Programmed gRNA scaffold for streamlined scRNA-seq in CRISPR screen (Direct-seq). 3rd generation lentiviral backbone.

   Depositor

   Lijia Ma

   Article

   Song et al Genome Biol. 2020 Jun 8;21(1):136. doi:

   Insert

   S. pyogenes sgRNA cassette (tRNA-Tetra-8A8G)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   hU6

   Available Since

   May 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFS_1064_pET30_pCat-tetR-Term-ptetA-FsRT-Cas1(opt)-Cas2(opt)-3xTerm_J23106-Leader-ARRAY2-DR2-FaqI_Term_NotI

   Plasmid

   #184739

   Purpose

   encodes aTc inducible FsRT-Cas1–Cas2 expression cassette and FsCRISPR Array 2 transcribed by BBa_J23106

   Depositor

   Randall Platt

   Article

   Schmidt et al Science. 2022 May 13;376(6594):eabm6038.

   Insert

   FsRT-Cas1(opt)-Cas2(opt)
   

   Expression

   Bacterial

   Mutation

   sequence codon optimized for expression in E. coli

   Promoter

   pTetA

   Available Since

   May 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. sgRNA2_IL1RN

   Plasmid

   #64142

   Purpose

   Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA2. Also contains a CMV-puro-t2A-mCherry expression cassette

   Depositor

   Moritoshi Sato

   Article

   Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

   Insert

   sgRNA2_IL1RN (IL1RN Human)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. sgRNA4_IL1RN

    Plasmid

    #64152

    Purpose

    Photoactivatable transcription system. Lentiviral expression of IL1RN sgRNA4. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA4_IL1RN (IL1RN Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. sgRNA1_NANOG

    Plasmid

    #64153

    Purpose

    Photoactivatable transcription system. Lentiviral expression of NANOG sgRNA1. Also contains a CMV-puro-t2A-mCherry expression cassette

    Depositor

    Moritoshi Sato

    Article

    Nihongaki et al Chem Biol. 2015 Feb 19;22(2):169-74. doi

    Insert

    sgRNA1_NANOG (NANOG Human)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    April 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pENTR-AtTAS1c-D2-B/c

    Plasmid

    #137883

    Purpose

    Gateway-compatible entry vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor at position downstream 3'D1[+]

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Insert

    AtTAS1c-D2-B/c
    

    Use

    Gateway-compatible entry clone

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Available Since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pEcRNAP5

    Plasmid

    #128939

    Purpose

    pET21a derivative encoding E. coli core RNA polymerase subunits: RpoAppx234-241-(His)10, B, C, Z

    Depositor

    Seth Darst

    Article

    Twist et al Protein Sci. 2011 Jun;20(6):986-95. doi:

    Inserts

    RpoAppx234-241-(His)10 (rpoA Escherichia coli)
    RpoB
    RpoC
    RpoZ
    

    Tags

    His

    Expression

    Bacterial

    Mutation

    Residues 234-241 are substituted for a PreScissio…

    Promoter

    T7

    Available Since

    Aug. 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLC-Flag-UBE2G1 sg2R-P2A-Hygro

    Plasmid

    #124299

    Purpose

    Lentiviral vector for expression of Flag tagged UBE2G1-P2A-Hygro casette from a CMV promoter. UBE2G1 cDNA contains silent mutations to disrupt a sgRNA binding site.

    Depositor

    Eva Gottwein

    Article

    Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

    Insert

    UBE2G1 (UBE2G1 Human)
    

    Use

    Lentiviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    several silent mutations to disrupt sgRNA targeti…

    Promoter

    CMV

    Available Since

    July 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAC8_MF-PH-Flag-Nter (VE5627)

    Plasmid

    #139773

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an N-ter Flag tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal Flag tag
    

    Tags

    Flag

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pAC8_MF-PH-HA-Cter (VE5629)

    Plasmid

    #139775

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter HA tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal Hemaglutinine tag
    

    Tags

    HA

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAC8_MF-PH-6His-Cter (VE5630)

    Plasmid

    #139777

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter6His tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal 6His tag
    

    Tags

    6 His

    Expression

    Insect

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC8_MF-PH-c-Myc-Cter (VE5632)

    Plasmid

    #139778

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a C-ter C-Myc tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal C-Myc tag
    

    Tags

    c-Myc

    Expression

    Insect

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAC8_MF-pH-Flag-Cter (VE5739)

    Plasmid

    #161799

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with an C-ter Flag tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    C-terminal Flag tag
    

    Tags

    Flag

    Expression

    Insect

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAC8_MF-PH-HA-Nter-P10-mCherry (VE5740)

    Plasmid

    #161800

    Purpose

    Transfer vector for multigene expression to generate recombinant baculoviruses by homologous recombination. Contains a p10/pH dual expression cassette with a N-ter HA tag under the pH promoter.

    Depositor

    Arnaud Poterszman

    Article

    Kolesnikova et al Sci Rep. 2022 Feb 7;12(1):2030. doi: 10.

    Insert

    N-terminal HA tag
    

    Tags

    HA

    Expression

    Insect

    Promoter

    PH

    Available Since

    May 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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