Skip to main content
Addgene
Showing: 1521 - 1560 of 4899 results

  2. pSelAct-KO

    Plasmid
    #174374
    Purpose
    Plasmid for unmarked deletions, pUC19 backbone, Apr resistant casette, codA::app counterselection casette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Vector for 2-step knockout by homologous recombin…
    Tags
    Expression
    Mutation
    Promoter
    N/A
    Available since
    March 7, 2022
    Availability
    Academic Institutions and Nonprofits only
    174374-plasmid-map-sequence-id-356074

    Enlarge

  6. pSelAct-Express

    Plasmid
    #174375
    Purpose
    Plasmid for integrative expression, pUC19 backbone, Apr resistant casette, pCMP1::eGFP expression cassette, gateway compatible
    Depositor
    Gitta Coaker
    Article
    Stevens et al Mol Plant Microbe Interact. 2021 Dec;34(
    Type
    Empty backbone
    Use
    Expression vector for targeted integration
    Tags
    Expression
    Mutation
    Promoter
    pCMP1
    Available since
    Sept. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    174375-plasmid-map-sequence-id-370623

    Enlarge

  7. pMT440

    Plasmid
    #8609
    Depositor
    Robert Hartley
    Article
    Yazynin et al Gene 1996 Feb 22;169(1):131-2.
    Insert
    barnase, barstar
    Use
    Tags
    as pMT416
    Expression
    Bacterial
    Mutation
    V36 codon of barnase of pMT416 replaced by polyli…
    Promoter
    Available since
    Jan. 17, 2007
    Availability
    Academic Institutions and Nonprofits only

  10. pHB1

    Plasmid
    #204668
    Purpose
    pUC19 containing the LL-FRT-erm-FRT-spacer sequence which serves as template for BarSeq deletion constructs
    Depositor
    Mark Mandel
    Article
    Burgos et al mSystems. 2020 Dec 15;5(6):e00846-20. do
    Insert
    FRT-erm-FRT
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 15, 2024
    Availability
    Academic Institutions and Nonprofits only
    204668-plasmid-map-sequence-id-422775

    Enlarge

  13. pCVD434

    Plasmid
    #63757
    Purpose
    EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae probe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  18. pCPP6534

    Plasmid
    #128735
    Purpose
    Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal HA tag
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 5, 2019
    Availability
    Academic Institutions and Nonprofits only
    128735-plasmid-map-sequence-id-252498

    Enlarge

  20. pCPP6533

    Plasmid
    #128734
    Purpose
    Gateway destination pUC18R6KT-Tn7T-PavrPto-GW-Cya-Km vector for integration into DC3000D36E genome glmS-linked attTn7 site any effector gene, expressed from avrPto promoter and with C-terminal Cya tag
    Depositor
    Alan Collmer
    Article
    Wei et al Cell Rep. 2018 May 8;23(6):1630-1638. do
    Type
    Empty backbone
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Aug. 6, 2019
    Availability
    Academic Institutions and Nonprofits only
    128734-plasmid-map-sequence-id-252576

    Enlarge

  29. pAV10

    Plasmid
    #63213
    Purpose
    pUC19 backbone (Amp), contains an RFP flanked by BsaI sites with CAGT & TTTT overhangs for TU assembly using yGG. NotI or FseI sites for TU digest.
    Depositor
    Jef Boeke
    Article
    Agmon et al ACS Synth Biol. 2015 Jul 17;4(7):853-9.
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Availability
    Academic Institutions and Nonprofits only
    63213-plasmid-map-sequence-id-113915

    Enlarge

  31. pAGT8163 (AATG_ttLbCas12a-i_TTCG)

    Plasmid
    #192453
    Purpose
    intronized ttLbCas12a for in planta expression
    Depositor
    Tom Schreiber
    Article
    Schindele et al Plant Biotechnol J. 2023 Mar;21(3):457-4
    Insert
    temperature-tolerant LbCas12a with introns (BsaI-flanked Golden Gate module, MoClo L0)
    Use
    CRISPR and Synthetic Biology; Moclo compatible le…
    Tags
    Expression
    Mutation
    temperature-tolerant mutation (D156R)
    Promoter
    Available since
    Dec. 6, 2022
    Availability
    Academic Institutions and Nonprofits only
    192453-plasmid-map-sequence-id-379029

    Enlarge

  34. pNAD1

    Plasmid
    #154347
    Purpose
    pUC19 backbone with editing template for homologous recombination mediated deletion of Nitrate reductase gene of N. oceanica and selection based on zeocin resistance
    Depositor
    John van der Oost
    Article
    Naduthodi et al Biotechnol Biofuels. 2019 Mar 25;12:66.
    Insert
    ShBle
    Use
    Microalgae
    Tags
    Expression
    Bacterial
    Mutation
    none
    Promoter
    Violaxanthin Chlorophyll a Binding Protein Precur…
    Available since
    Nov. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    154347-plasmid-map-sequence-id-316607

    Enlarge

  38. loxP-G418-loxP-3xALFA-Halo-Rab11 HR

    Plasmid
    #229676
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with Halotag and 3x ALFA tag. Contains a G418 resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    Halo, 3xALFA
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229676-plasmid-map-sequence-id-457076

    Enlarge

  39. loxP-Puro-loxP-3xV5-SNAP-Rab11 HR

    Plasmid
    #229677
    Purpose
    Homology repair plasmid for endogenous tagging of Rab11 at the N-terminus with SNAP tag and a 3x V5 epitope tag. Contains a puromycin resistance cassette for selection of edited cells.
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence Rab11 locus (RAB11A Human)
    Use
    CRISPR
    Tags
    SNAP, 3xV5
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229677-plasmid-map-sequence-id-457129

    Enlarge

  40. AP1muA-mStayGold-polyA-Hygro HR

    Plasmid
    #229679
    Purpose
    Homology repair plasmid for endogenous tagging of AP1muA at the C-terminus with monomeric StayGold. Contains a hygromycin resistance cassette for selection of edited cells
    Depositor
    Francesca Bottanelli
    Article
    Stockhammer et al Nat Cell Biol. 2024 Oct 4. doi: 10.1038/
    Insert
    genomic sequence AP1M1 locus (AP1M1 Human)
    Use
    CRISPR
    Tags
    monomeric StayGold
    Expression
    Mutation
    Promoter
    Available since
    Jan. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    229679-plasmid-map-sequence-id-457128

    Enlarge

Showing: 1521 - 1560 of 4899 results